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Custom Peptide Nucleic Acid Synthesis

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CD Formulation produces high-quality PNAs by customizing products in various lengths, shapes, and specifications. We have quantity parameters like 2 OD, 5 OD, 10 OD, milligrams and grams, with flexible synthesis parameter choices.

Background of Peptide Nucleic Acid (PNA)

PNA is a DNA-like and RNA-like synthetic polymer, composed of purine and pyrimidine groups attached to a peptide-like scaffold via methylenecarbonyl bonds. PNA, with its no charged phosphate groups, is less strongly repelled by electrostatic repulsion than DNA. Additionally, PNA is a non-toxic against DNases and proteases, which renders it extremely stable in the body and in vitro.

Fig.1 Characteristics of Peptide Nucleic Acid. (CD Formulation)

PNA Design and Considerations

Length

PNA's high affinity allows for shorter sequences of 12-18 bases, a notable shift from the standard 25 to 40 base oligonucleotide probes.

Purine Content

Do not include six or more purines in any sequence of ten consecutive PNA monomers.

Self-Complementarity

Avoid inverse repeats, hairpin-forming structures, and palindromic sequences in your sequence design.

Enhancing Solubility

To enhance the solubility of PNA probes, co-solubilizing groups such as the O linker, E linker, X linker, or two lysine residues can be incorporated into the sequence.

Explore Our Custom PNA Synthesis Services

CD Formulation provides customized PNA synthesis, creating tailored PNA sequences to meet clients' specific needs. This enhances the accuracy and efficiency of research and clinical applications.

Chemical Modification Services for PNA

Items	Descriptions
Hydroxymethyl-PNA Synthesis	By incorporating a hydroxymethyl group at the Cγ position of the N-(2-aminoethyl)-glycine unit in the PNA backbone, the modified γ-PNA exhibits enhanced binding affinity to both DNA and RNA.
Diethylene Glycol-PNA Synthesis	In addition to hydroxymethyl groups, we can incorporate diethylene glycol side chains into the peptide backbone to enhance the binding strength of the PNA and introduce modified PNAs.
PNA-CPP Synthesis	Conjugation to Cell-Penetrating Peptides (CPPs)
PNA-DNA/RNA Synthesis	Conjugation to DNA and RNA
Fluorescent PNA Synthesis	Conjugation to a wide range of fluorescent dyes (such as fluorescein, rhodamine, and cyanines) at one end.
Biotinylated PNA Synthesis	Conjugation of biotin at one end.
Alkyne/Azide-PNA Synthesis	Incorporation of an alkyne or azide functional group at one end.

These chemical modification services can effectively address the challenge of delivering PNA candidates to target cells when needed.

Quality Control Measures

CD Formulation strictly controls the synthesis of PNA using LC/MS and provides a certificate of analysis, which includes the LC and MS reports along with solubility conditions.

Our Workflow for Peptide Nucleic Acid (PNA) Preparation

Fig.2 Workflow for Peptide Nucleic Acid (PNA) preparation Fig.2 Flow chart of PNA preparation. (CD Formulation)

PNA Design

At this stage, researchers determine the specific sequence and length of the peptide nucleic acid and its intended function. The design considers the target binding properties and potential chemical modifications.

PNA Synthesis

PNA is typically synthesized using solid-phase synthesis, where the PNA chain is constructed through the stepwise addition of amino acid units. Liquid-phase synthesis can also be employed and is suitable for small-scale synthesis requirements.

PNA Deprotection

Upon completion of the synthesis, the protective groups must be removed to release the full PNA chain. This step is essential for ensuring the activity and functionality of the PNA.

Purification

PNA is purified via HPLC to remove unreacted materials and by-products, aiming for high purity for future applications.

Characterization

Finally, the structure and purity of PNA are verified using techniques such as MS and NMR. This verification ensures that the synthesized PNA meets the design specifications and exhibits the desired biological activity.

Our Technology Platforms

Solid-phase Synthesis Technology

CD Formulation offers a synthesis technology platform, including solid-phase synthesis, characterized by high efficiency and accuracy to ensure the quality and purity of PNA products. Our platform not only supports the synthesis of standard sequences but also meets specific customer needs, enabling the realization of complex PNA designs through flexible synthesis strategies.

Analytical Technology Platforms

CD Formulation's analytical technology platform incorporates advanced capabilities to ensure the quality and purity of each peptide nucleic acid product. The platform employs various modern analytical techniques, such as HPLC and MS, to deliver comprehensive product analysis reports for clients, meeting the stringent requirements for different research or application needs.

Highlights of Peptide Nucleic Acid (PNA) Synthesis Services

Multi-Scale synthesis (Nanomolar to Millimolar).
Comprehensive quality control analysis using HPLC and MALDI-TOF mass spectrometry.
Ability to manage projects of all sizes.
A clear commitment to long-term continuous improvement and competitive pricing.
High throughput, rapid delivery.

Publication Data

Technology: Peptide nucleic acid (PNA) preparation by chemical synthesis technology

Journal: European journal of human genetics

IF: 5.2

Published: 2004

Results:

Peptide nucleic acids (PNAs) are synthetic mimics of DNA in which the deoxyribose phosphate backbone is replaced by a pseudopeptide polymer to which the nucleobases are attached.PNAs hybridize with complementary DNA or RNA with very high affinity and specificity, primarily because of their uncharged and flexible polyamide backbone.The unique physicochemical properties of PNAs have led to the development of a variety of research and assay methods. The unique physicochemical properties of PNAs have led to the development of a variety of research assays, and the use of PNAs over the past few years has demonstrated their power in molecular biology procedures and diagnostic assays. As a result, several sensitive and robust PNA-dependent methods have been devised for the development of antigenic and anticancer drugs, the modulation of PCR reactions, the detection of genomic mutations or the in situ labeling of chromosomes.

Fig.3 Peptide Nucleic Acid (PNA) Fig.3 Chemical structures of PNA as compared to DNA and protein.

CD Formulation will fulfill your PNA needs through cost-effective synthesis. We offer a diverse range of sizes, scales, and various modifications. Contact us at any time, and our dedicated team of chemists and biosynthetic experts will assist you in developing the optimal design and synthesis strategy to achieve your research objectives.

References

Pellestor F, Paulasova P. The peptide nucleic acids (PNAs), powerful tools for molecular genetics and cytogenetics. EUR J HUM GENET. 2004, 12(9): 694-700.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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