Polymerase Chain Reaction (PCR) Technology Platform for Nucleic Acid Drugs

CD Formulation offers a PCR technology platform that focuses on the efficient and accurate analysis of nucleic acid preparations. Our platform integrates the latest advancements in polymerase chain reaction technology to sensitively detect and quantify nucleic acid sequences in samples, delivering reliable data for research and development as well as clinical studies.

About Polymerase Chain Reaction (PCR) Technology

Polymerase Chain Reaction (PCR) is a revolutionary molecular biology technique used to amplify specific fragments of DNA, enabling researchers to generate millions to billions of copies of a target DNA sequence from a small amount of starting material. The primary process of PCR involves the binding of a small, synthetic, single-stranded DNA fragment known as a primer to a specific region of the template DNA, utilizing four deoxynucleotide triphosphates (dNTPs) as substrates. The goal is to amplify DNA in vitro by polymerizing the primer with the corresponding region of the template DNA. DNA polymerase then extends the DNA fragments along the 3' end of the double-stranded region formed by the primer and the template. PCR has revolutionized the fields of biology, genetics, forensic science, and medicine due to its speed, specificity, and versatility.

Steps of PCR Reaction

Fig.1 Steps of PCR Reaction. (CD Formulation)

• Denaturation - The process of denaturing DNA double strands into single-stranded DNA through heating.
• Annealing - The reaction mixture is cooled to an appropriate temperature, and the two primers are combined with the complementary regions of the two single-stranded templates.
• Elongation - The temperature of the reaction is increased once more, facilitated by DNA polymerase and the presence of Mg²⁺. Four types of deoxynucleotide triphosphates (dNTPs) begin to incorporate from the 3' end of the primer, allowing the primer to extend in the 5' to 3' direction and synthesize a new complementary DNA strand.

Types of Polymerase Chain Reaction (PCR) Technology Platforms

CD Formulation provides a comprehensive array of polymerase chain reaction technology platforms for analyzing nucleic acid preparations. Our platform encompasses various types of PCR technologies to accommodate the diverse requirements of bioanalytical projects.

Fluorescent Quantitative PCR Platform

Fluorescence quantitative PCR involves the incorporation of fluorescent groups into the reaction system. This technique utilizes fluorescent signals to monitor the PCR process in real time and allows for the quantitative analysis of the initial template through the use of a standard curve.

Reverse Transcription PCR Platform

Reverse transcription PCR (RT-PCR) utilizes RNA as a template. First, reverse transcriptase is employed to convert the RNA into complementary DNA (cDNA). Subsequently, the cDNA serves as the template for PCR amplification.

RACE-PCR Platform

RACE, or Rapid Amplification of cDNA Ends, is a technique used to obtain the 5' and 3' ends of a complete cDNA from a known partial cDNA sequence.

Reverse PCR Platform

Reverse PCR utilizes reverse complementary primers to amplify fragments of unknown sequences that extend beyond the two primers. In contrast, conventional PCR amplifies the fragments located between the two primers.

Falling PCR Platform

Falling PCR is the process of optimizing the annealing temperature in a PCR reaction system by establishing a series of progressively decreasing annealing temperatures. This approach enhances the specificity of the PCR reaction.

Nested PCR Platform

Nested PCR involves two rounds of PCR amplification utilizing two sets of primers. This consecutive amplification enhances both the specificity and sensitivity of the results.

Highlights of Polymerase Chain Reaction Platform for Nucleic Acid Drugs

• Sensitivity - PCR can detect trace amounts of DNA, which makes it highly sensitive for detecting low copy number targets.
• Specificity - By utilizing specific primers, PCR can selectively amplify target DNA sequences, even within complex mixtures that contain other DNA.
• Fast - PCR reactions can be completed in just a few hours, enabling the rapid amplification and analysis of DNA samples.
• Versatility - PCR can be utilized for a wide range of applications, including genotyping, gene expression analysis, DNA sequencing, and mutation detection.
• Robustness - PCR is a highly robust technique that accommodates variations in reaction conditions and sample types, making it widely applicable in various research and diagnostic settings.

Our Analytical Characterization Services

CD Formulation's PCR technology accurately identifies target sequences and provides reliable quantitative and qualitative information, assisting researchers and clinicians in performing precise nucleic acid analysis and diagnosis.

Publication Data

CD Formulation's polymerase chain reaction (PCR) technology platform is an efficient and sensitive tool for nucleic acid analysis. With rapid reaction times, user-friendly operating procedures, and robust data analysis capabilities, our platform delivers reliable results for both research and clinical applications. Please feel free to contact us, and we will provide you with professional solutions.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.