Infrared Spectroscopy Platform for Nucleic Acid Drugs

CD Formulation is dedicated to delivering advanced infrared spectroscopy solutions for drug discovery and development, enabling researchers to gain deeper insights into and analyze a wide range of complex systems. With our state-of-the-art infrared spectroscopy platform, customers can conduct comprehensive nucleic acid structure identification, monitor dynamic changes, perform quantitative analysis, and carry out spectral analysis.

Background of Infrared Spectroscopy for Nucleic Acid Drugs

Infrared spectroscopy is a crucial technique for studying the structure and chemical bonding of molecules, as well as for characterizing and identifying chemical species. Infrared (IR) spectra are generated by analyzing the absorption patterns of infrared light by a substance, which correspond to the vibrations of chemical bonds and atomic groups within a molecule. For nucleic acid molecules, such as DNA and RNA, IR spectroscopy can provide valuable insights into changes in their secondary structure and dynamics. By examining the infrared spectrograms of nucleic acids, specific chemical bonds and functional groups - such as phosphodiester bonds (P=O) and sugar rings in nucleotides - can be identified, enhancing our understanding of their structural properties.

Fig.1 Analysis of nucleic acids. (Ghimire H, et al., 2017)

Our Solutions of Infrared Spectroscopy Platform for Nucleic Acid Drugs

CD Formulation provides infrared spectroscopy platforms with professional technical support to provide customized solutions to help clients analyze the identification and analysis of nucleic acid molecules during drug development.

Fig.2 Our solutions of infrared spectroscopy platform. (CD Formulation)

Spectral Analysis of Nucleic Acids

Infrared spectroscopy typically displays transmittance or absorbance as a function of wavenumber. Specific chemical bonds and functional groups in biomolecules, such as nucleic acids, generate characteristic absorption peaks in the spectra. For example, the phosphodiester group (P=O) produces absorption peaks in the range of approximately 1220-1250 cm^-1 and 1080-1100 cm^-1, corresponding to symmetric and asymmetric P=O stretching vibrations, respectively.

Structure Recognition of Nucleic Acids

By analyzing the characteristic peaks in the infrared spectra, it is possible to identify specific functional groups and chemical bonds present in the sample. This analysis can also aid in determining the secondary structure of biomolecules. For instance, in nucleic acids, the secondary structures of DNA and RNA (such as the double helix, hairpin, and G-quadruplex structures) exhibit distinctive absorption patterns in the infrared spectrograms that correspond to specific chemical bonds and spatial configurations. Absorption peaks at particular wavelengths, such as those associated with the stretching vibrations of phosphodiester bonds and the hydrogen bonding vibrations between base pairs, can further facilitate the analysis of the specific structures of nucleic acids and their conformational states.

Dynamic Changes Analysis of Nucleic Acids

Dynamic changes in nucleic acids can be analyzed using infrared spectroscopy to reveal their conformational alterations and intermolecular interactions under various conditions. For example, DNA transitions from a double helix structure to a single-stranded form during melting, which can be observed by monitoring the changes in specific peaks of the IR spectrum. Additionally, RNA exhibits characteristic changes in IR absorption peaks during folding and the formation of complex secondary structures, such as hairpin loops and stem-loop structures. By tracking these absorption peaks in real time, researchers can gain a comprehensive understanding of how nucleic acids regulate their functions and fulfill their biological roles during dynamic processes.

Quantitative Analysis of Nucleic Acids

Although IR spectroscopy is primarily utilized for qualitative analysis, it can also serve quantitative purposes. By comparing the absorption peaks of an unknown sample with those of a sample of known concentration (particularly in specific bands, such as those associated with phosphate groups) the concentration of the unknown sample can be accurately determined. This method has practical applications in determining nucleic acid concentrations, quantitative PCR and gene expression analysis.

Highlights of Infrared Spectroscopy Platform for Nucleic Acid Drugs

• Infrared spectroscopy can identify specific chemical bonds and functional groups within molecules, offering highly detailed information. This technique is particularly valuable for analyzing the structure of complex biomolecules.
• This technology enables real-time monitoring of dynamic changes in biomolecules, such as alterations in nucleic acid structure, thereby revealing intricate details of intermolecular interactions.
• Samples can be analyzed without destructive treatment, thereby preserving their natural state.
• Not only can qualitative analysis be conducted, but quantitative analysis can also be performed by comparing peak intensities to determine the concentration of the sample.

Our Analytical Characterization Services

CD Formulation's infrared spectroscopy platform is an efficient and precise tool for accurately analyzing the molecular structure and composition of nucleic acid drugs, ensuring that their purity and quality meet stringent standards.

Publication Data

The advanced infrared spectroscopy platform offered by CD Formulation enables precise resolution of structural and dynamic changes in biomolecules. From monitoring dynamic alterations in nucleic acids to conducting quantitative analyses, our platform delivers a comprehensive solution to support bioscience research and drug development. Contact us for expert project solutions.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.