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Dynamic Light Scattering (DLS) Platform for Nucleic Acid Drugs

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CD Formulation's Dynamic Light Scattering (DLS) technology platform offers a comprehensive analytical solution for nucleic acid formulations. Equipped with highly sensitive detectors and advanced data analysis systems, we provide critical information regarding particle size, shape, and diffusion. Our DLS technology presents significant advantages, including short analysis times and minimal sample preparation requirements, enabling our customers to save time and resources while accelerating the development process of nucleic acid formulations.

Our Advantages of Dynamic Light Scattering (DLS) Platforms

Dynamic Light Scattering (DLS) is a well-established biophysical technique for accurately characterizing particle size in suspensions and emulsions. Widely recognized in both the pharmaceutical and industrial sectors, DLS optically measures motion by recording a scattered light signal at a fixed angle. A monochromatic, coherent light source illuminates the particles, and the light scattered by these particles is subsequently recorded.

Fig.1 Advantages of DLS technology platforms. (CD Formulation)

Fast and Accurate Measurement - Comprehensive data is accessible in under a minute.
Nanoscale Range - Measure particles from 0.5 nm to 2.5 µm with high resolution.
Non-Invasive Analysis - Preserve sample integrity through non-invasive and recoverable analysis.
Minimum Sample Size - Use smaller sample sizes to enhance efficiency.
Versatility - Assess stability, aggregation, molecular interactions, and additional factors.
Pre-Screening Efficiency - This method is ideal for assessing sample quality before utilizing costly biophysical techniques.
Hydrodynamic Radius and Size Distribution - This section provides detailed information ranging from 1 nm to 10 µm.
Comprehensive Physical Analysis - This section covers critical micelle concentration, gelation, aggregation, and additional relevant topics.
Electrokinetic Potential Measurements - Determining Particle Charge and Stability.

Our Solutions of DLS Platform for Nucleic Acid Drugs

CD Formulation is dedicated to delivering cutting-edge DLS solutions for the characterization of nucleic acid formulations. Our DLS technology platform serves several key functions in the analysis of these formulations.

Particle and Molecular Size Determination	Conformational Change Analysis
DLS facilitates the rapid measurement of particle size and distribution of nucleic acid molecules, as well as the identification of monomers, dimers, and polymers in nucleic acid formulations. It also allows for quantitative analysis of aggregation in samples. By determining the particle size and polydispersity index (PDI), we can detect the presence of aggregates or large particles that may impact drug delivery efficiency.	DLS can provide insights into the conformation of nucleic acid molecules by measuring their hydrodynamic radius. Analyzing conformational changes allows for a better understanding of the unfolding process, alterations in secondary structure, and the conformational formation of nucleic acids under various environmental conditions or processing scenarios. Additionally, it can detect subtle changes that may influence their functional properties.
Nanoparticle and Carrier Evaluation	Stability Analysis
DLS technology excels at measuring the particle size distribution and stability of nucleic acid formulation carriers, such as liposomes and nanoparticles, thereby facilitating the development of efficient delivery systems.	DLS evaluates the stability of nucleic acid formulations by monitoring changes in particle size and distribution over time. Our experts offer insights into the stability characteristics of these formulations by quantifying variations in particle size and increases in aggregation, which aids in optimizing storage conditions and extending shelf life.

Highlights of DLS Platform for Nucleic Acid Drugs

Capable of performing not only conventional size distribution analysis but also integrating it with other characterization tools, such as electrophoretic light scattering, to provide more comprehensive information about sample properties.
Quantitative analysis utilizing advanced DLS technology to evaluate the aggregation state and stability of antibody drugs by measuring the hydrodynamic radius, polydispersity index, and particle concentration.
The ability to monitor dynamic changes in a sample in real time provides valuable data for studying the stability and behavior of nucleic acid drugs under various conditions.
The use of a highly sensitive detector allows for the detection of low-concentration samples and tiny particles, facilitating more precise analysis.

Our Analytical Characterization Services

CD Formulation has developed a highly sensitive and efficient DLS platform that offers a comprehensive solution for the characterization of nucleic acid formulations. By conducting in-depth analyses of the various properties of nucleic acid particles, we assist our customers in optimizing their drug formulations to ensure high quality and stability, thereby accelerating the development and commercialization of nucleic acid drugs.

Particle Size Analysis for Nucleic Acid Drugs

Our DLS platform facilitates the rapid and efficient measurement of the particle size distribution of nucleic acid formulations. By analyzing the hydrodynamic radius and polydispersity index (PDI), we can fully characterize the size and homogeneity of nucleic acid particles.

Aggregation Analysis for Nucleic Acid Drugs

The DLS technique enables a detailed analysis of the aggregation state of nucleic acid drugs. By identifying the presence of aggregates and oligomers in the sample, we can evaluate factors that may impact the drug's safety and efficacy.

Stability Analysis for Nucleic Acid Drugs

Our DLS platform also offers stability assessments for nucleic acid drugs. By monitoring real-time changes in nucleic acid particle size and distribution, we can evaluate the stability of a drug during both storage and application.

Publication Data

Technology: Dynamic Light Scattering technology platform for therapeutic nanoparticles

Journal: RSC advances

IF: 4.036

Published: 2020

Results:

The authors designed transferrin (Tf)-functionalized p(HEMA-ran-GMA) nanoparticles for the incorporation and release of a water-soluble combination of three ion channel antagonists, namely, zolazanammonium monohydrate (YM872), oxidized adenosine triphosphate (oxATP), and lomerizine hydrochloride (LOM), which are considered to be effective for the treatment of secondary degeneration after neurotrauma Therapy. Under representative physiological conditions, all three drugs were co-released from Tf-functionalized p(HEMA-ran-GMA) nanoparticles and assayed for up to 20 minutes. Taken together, this study contributes insight into potential physiological outcomes in the presence of serum proteins and suggests that p(HEMA-ran-GMA)-based therapeutic nanoparticles may be an ideal delivery vehicle for CNS therapy.

Fig.2 Dynamic Light Scattering technology platform for therapeutic nanoparticles Fig.2 Nanoparticle characterization by Dynamic Light Scattering (DLS). (Naidu P S R, et al., 2020)

CD Formulation's experienced nucleic acid formulation analysts will evaluate the suitability of your samples and project for DLS analysis, explaining how this analytical technique can yield valuable insights for your project. Customers can select from a range of technology platforms tailored to their specific project requirements, please contact us, and our team of experts will develop a customized experimental process for you.

References

Naidu P S R, Denham E, Bartlett C A, et al. Protein corona formation moderates the release kinetics of ion channel antagonists from transferrin-functionalized polymeric nanoparticles. RSC Adv. 2020, 10(5): 2856-2869.

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STEP 2

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STEP 3

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