Circular Dichroism (CD) Platform for Nucleic Acid Drugs

CD Formulation's Circular Dichroism Platform is utilized to measure the circular dichroism spectra of specific molecules across a defined wavelength range. Our platform can be employed to study chiral molecules, investigate the secondary structure and configuration of macromolecules, and acquire kinetic and thermodynamic information about molecules under varying environmental conditions or interactions.

Our Advantages of Circular Dichroism (CD) Platforms

Circular dichroism (CD) is an absorption spectroscopy technique that measures the difference in absorption between left and right circularly polarized light. This method is utilized to assess how a substance interacts with these two types of polarized light. Nucleic acids exhibit regular secondary structures, and their folding and conformational changes can be effectively analyzed using CD spectroscopy. Specific nucleic acid structures, such as double and triple helices, along with their conformational states and purity, can be evaluated through their CD spectral bands within a defined wavelength range. CD spectroscopy offers several advantages for nucleic acid analysis:

• High Selectivity
• Efficient Analysis
• Easy to Operate
• Wide Range of Applications
• ...

Fig.1 Advantages of circular dichroism platforms. (CD Formulation)

Our Circular Dichroism (CD) Platform Types

CD Formulation utilizes a range of advanced circular dichroism platforms to analyze nucleic acid formulations, ensuring highly accurate and sensitive measurements. Below are the various circular dichroism platforms available.

Our Capabilities of CD Platform for Nucleic Acid Drugs

CD Formulation's circular dichroism platform offers a comprehensive solution for nucleic acid analysis, facilitating efficient and accurate assessments of their structural properties. Our platform specializes in examining the conformational changes of nucleic acids under various environmental conditions, assisting our customers in understanding the intricate folding and pairing mechanisms of these biomolecules.

Nucleic Acid Conformational Analysis

Circular dichroism (CD) technology is widely employed to investigate the secondary structure of nucleic acids, including double helix formation, folding, and bending. By analyzing the CD spectra of nucleic acids at various wavelengths, researchers can obtain valuable conformational information about nucleic acid molecules and observe how these structures change under different environmental conditions.

Nucleic Acid Thermal Stability Analysis

Circular dichroism spectroscopy can be employed to determine the thermal solubility curves of nucleic acids, thereby assessing their thermal stability. By gradually increasing the temperature and recording the corresponding changes in the CD spectra, the melting temperature (Tm) of the nucleic acid can be identified.

Ligand Binding Analysis

Circular dichroism can be utilized to investigate the interactions between nucleic acids and small molecule ligands, such as drug molecules. By analyzing the changes in the circular dichroism spectra upon ligand binding, researchers can infer the binding site, binding constants, and the effects on the structure of the nucleic acid.

Dynamic Change Monitoring Analysis

Circular dichroism technology offers a method for real-time monitoring of dynamic changes in nucleic acids. By swiftly scanning CD spectra, researchers can capture conformational changes in nucleic acids at various time points, including complexation, deconvolution, and ligand binding processes.

Quantitative Analysis

CD spectroscopy can also be utilized for the quantitative analysis of nucleic acids. By measuring the absorption intensity at a specific wavelength, the concentration of nucleic acids in a sample can be determined.

Highlights of CD Platforms for Nucleic Acid Drugs

• Our team of experienced biophysicists conducts precise measurements of your samples using a state-of-the-art CD spectrometer. By analyzing the differential absorption of left-circularly polarized light and right-circularly polarized light, we can reveal valuable information about the secondary structure composition and conformational changes of biomolecules.
• Whether you are studying nucleic acids or other chiral molecules, our circular dichroism (CD) spectroscopy service is suitable for a diverse array of sample types. From small molecules to macromolecular complexes, we tailor our methods to meet your specific research requirements.
• Our instruments are optimized for high sensitivity and resolution, enabling us to detect subtle structural changes in samples. This capability is particularly crucial when monitoring protein folding, ligand binding, and conformational changes.
• In addition to fundamental structural information, our CD spectroscopy service offers quantitative data, including the percentage of secondary structure composition. This quantitative insight is crucial for precise comparative analysis and mechanistic studies.

Our Analytical Characterization Services

CD Formulation's circular dichroism (CD) platform offers an effective tool for investigating the structure and function of nucleic acid drugs.

Publication Data

CD Formulation offers a cutting-edge technology platform for analyzing nucleic acid molecular structures, molecular chirality, and intermolecular interactions. Our team of experts collaborates with you to design tailored experiments that yield relevant and valuable data. If you are interested in our services, please feel free to contact us for a detailed quote.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.