Gas Chromatography (GC) Platforms for Nucleic Acid Drugs

CD Formulation's gas chromatography (GC) technology platforms deliver high-quality data and results by harnessing the benefits of high resolution, high sensitivity, and rapid detection and analysis of nucleic acid molecules. Our gas chromatography platforms are utilized in various applications, including the detection of nucleic acid molecules, drug metabolism studies, and environmental sample analysis.

Our Solutions of GC Platforms for Nucleic Acid Drugs

CD Formulation's gas chromatography platform offers innovative and comprehensive solutions tailored to meet our customers' needs in the analysis of nucleic acid molecules.

Analysis of Nucleic Acid Bases and Their Modifiers

Bases and their modifiers in nucleic acid molecules can be derivatized to enhance their volatility, rendering them suitable for gas chromatographic analysis.

Identification of Nucleic Acid Derivatives

Gas chromatography offers significant advantages for analyzing compounds with low molecular weights and high volatility. Additionally, chemical derivatization can be employed to transform nucleic acid degradation products into compounds that are suitable for GC analysis.

Analysis of Single Nucleotides and Their Metabolites

When studying the metabolic pathways of nucleic acids, it is necessary to derivatize samples of single nucleotides and their metabolites for GC analysis.

Our Workflow of GC Platforms for Nucleic Acid Drugs

Fig.1 Flowchart for detecting nucleic acid molecules. (CD Formulation)

Sample Preparation

The sample preparation process must ensure that both purity and concentration meet the experimental requirements. This typically involves extracting nucleic acids from the biological sample and eliminating impurities through a purification step.

Derivatization

Since nucleic acid molecules in their natural state are not well-suited for GC analysis, chemical derivatization - such as using silylation or amination reagents - is necessary to convert the nucleic acids into volatile compounds that are suitable for GC analysis.

Sample Injection

The derivatized nucleic acid sample is injected into the GC system using a sampler. The injection can be performed either through an autosampler or manually to ensure accurate placement of the sample into the chromatography system.

Detection

Nucleic acid derivatization products are introduced into the detector of the GC. Commonly used detectors include flame ionization detectors (FID) and mass spectrometers (MS).

Data Analysis

Acquired chromatogram and mass spectrometry data are analyzed by software. By comparing the experimental data with standard reference data, the content and structural information of each component in the sample can be accurately determined.

Highlights of GC Platforms for Nucleic Acid Drugs

• GC technology possesses exceptional resolving power and can effectively separate individual components within complex mixtures. Furthermore, the incorporation of highly sensitive detectors, such as Mass Spectrometry Detectors (MSD), can greatly enhance the detection of trace components.
• By utilizing derivatization, GC technology can be adapted for a diverse array of polar and non-polar nucleic acid molecules. This adaptation enhances volatility, facilitating improved detection and analysis.
• GC analysis is generally rapid, allowing for the separation and examination of complex samples within a relatively brief timeframe. This efficiency is especially crucial for time-sensitive research projects.
• Our platforms are equipped with cutting-edge instrumentation and software, delivering highly accurate and reproducible data for scientific research and product development.

Our Analytical Characterization Services

CD Formulation's platform offers precise and efficient analytical services, specializing in the analysis of nucleic acid drugs. Utilizing advanced GC technology, we ensure the purity and quality of nucleic acid drugs, providing dependable support for research, development, and manufacturing.

Publication Data

CD Formulation's GC platform specializes in the analysis and detection of nucleic acid molecules, dedicated to addressing the diverse needs of our customers in nucleic acid formulation project development. Feel free to contact us for efficient and reliable solutions for degradation product analysis, modified nucleic acid detection, and quality control.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.