Aptamer-siRNA Conjugate Development

CD Formulation develops innovative conjugates by combining aptamers with siRNA in the exploration of novel drug delivery systems. Leveraging the unique potential of aptamer-siRNA conjugates, CD Formulation is dedicated to advancing gene-targeting technologies and offering exclusive technical support to customers around the globe.

Why Combine Aptamers with siRNA?

Nucleic acid aptamers are small DNA or RNA segments crafted from random libraries through the SELEX method. With sizes between 6-30 kDa and diameters of 1-2 nm, they have low immunogenicity and flexible structures, ideal for interacting with small targets. Aptamers and siRNAs can form chimeras through covalent attachment or base complementation. Enhancing stability involves applying chemical modifications like changing the 3' and/or 5' ends to guard against hydrolysis, adjusting the 2' position of sugar components, or transforming phosphodiester links into thiophosphodiester ones to boost nuclease resistance.

Fig.1 Aptamer-siRNA AsiC designs. (Kruspe S, et al., 2017)

Advantages of Aptamer-siRNA Conjugates

Using siRNA-loaded nanocarriers with nucleic acid aptamers for targeted delivery offers multiple benefits.

• Nanocarriers linked with aptamers can precisely transport substances to specific cells.
• By encapsulating siRNA, nanocarriers provide protection against degradation.
• The delivery of siRNA is improved by nanomaterials, which also extend its presence in the system.
• For better treatment and imaging at the molecular level, nanomaterials can combine therapeutic elements and diagnostic probes.

Explore Our Services for Aptamer-siRNA Conjugate Development

Biosynthetic Aptamer-siRNA Conjugate Services

Biosynthetic methods typically utilize biological systems for synthesis. The advantage of this approach is its ability to produce molecules with natural post-translational modifications or intricate structures that are challenging to synthesize through conventional chemical methods. However, this process may necessitate longer and more complex production conditions.

Chemical Synthesis of Aptamer-siRNA Conjugates Services

The chemical synthesis method employs chemical reactions to gradually assemble nucleic acid molecules in vitro. This method offers high controllability and stable purity, making it particularly suitable for synthesizing shorter nucleic acid molecules with simpler structures. Additionally, the chemical synthesis technique enables precise modifications at specific sites within the molecular structure, contributing to its widespread use in research and development.

Workflow of Aptamer-siRNA Conjugate Development

Fig.2 Flow chart of aptamer-siRNA conjugate development. (CD Formulation)

• Aptamer Screening and Optimization

Through a series of in vitro selection techniques, such as SELEX, aptamers with a strong binding affinity for target molecules are screened and structurally modified to enhance binding specificity and stability.

• siRNA Sequence Design

Design siRNA sequences that are complementary to the target RNA, and evaluate their effectiveness using bioinformatics tools. This approach will ensure that the selected sequences can accurately target and silence the intended genes.

• Ligation Technology Development

Develop suitable chemical cross-linking methods to effectively link the aptamer to the siRNA, ensuring that the resulting complex possesses the necessary stability and activity in both its physical and chemical properties.

• Evaluation of Physicochemical Properties

The synthesized aptamer-siRNA complexes are analyzed for their detailed physicochemical properties, including solubility, stability, and particle size distribution, to ensure their suitability for subsequent applications.

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Advantages of Aptamer-siRNA Conjugate Development Services

• Aptamer-siRNA conjugation is designed to be flexible and can target a wide range of molecular targets.
• Optimized chemical modifications enhance stability and increase persistence in vivo.
• High binding efficiency and reduced immunogenicity are attributed to its smaller size and flexible structure.
• The synthesis and testing can be conducted quickly and efficiently using customized processes.
• Covalent linkage or base complementation is employed to create stable chimeras through advanced technical methods.

Published Data

CD Formulation combines cutting-edge technology with the expertise of dedicated scientists to offer you comprehensive support and innovative solutions. Contact us, our team consistently stays updated on the latest experimental findings and research developments.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.