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NMR Technology Platform for Nucleic Acid Drugs

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Nuclear Magnetic Resonance (NMR) technology is extensively utilized in nucleic acid drug research because of its non-destructive nature, high-resolution capabilities, and ability to acquire multi-dimensional information. CD Formulation, an advanced NMR technology platform, is committed to delivering comprehensive and precise molecular analysis services for nucleic acid drugs.

About Nuclear Magnetic Resonance Technology for Nucleic Acid Drugs

The principle of NMR is based on the behavior of magnetic nuclei, such as hydrogen atoms, in a magnetic field. When these nuclei are placed in a magnetic field, they produce subtle frequency differences that depend on their chemical environment, which can be detected by the NMR instrument. By analyzing these frequency differences, known as chemical shifts, researchers can infer the environment and relative positions of the atoms, providing valuable information about the molecular structure. Additionally, multidimensional NMR techniques allow for the detailed study of large molecules and complex systems. For nucleic acid molecules, NMR can yield comprehensive structural information, making it a powerful tool for characterizing the structures of various compounds.

Fig.1 Advantages of NMR technology platforms.Fig.1 Advantages of nuclear magnetic resonance technology platforms. (CD Formulation)

Types of NMR Technology

Types Descriptions
High-Field Strength NMR Technology High-field strength NMR can more accurately resolve the structures of complex molecules.
Multinuclear NMR Technology The multinuclear NMR technique can simultaneously detect multiple nuclides, including hydrogen, carbon, nitrogen, and oxygen. This capability provides more comprehensive information and aids in determining the structure of molecules with greater accuracy.
Heteronuclear NMR Technology Nuclear NMR techniques leverage interactions between different nuclides to obtain structural information, such as the carbon-hydrogen (¹³C-¹H) coupling constants, which are essential for elucidating molecular structures.

Our Solutions of NMR Technology Platforms

CD Formulation's NMR technology platform plays a crucial role in nucleic acid drug discovery. Through high-resolution NMR imaging and spectroscopic analysis, the platform delivers detailed information on molecular structure, dynamics, and interaction mechanisms. Our NMR platform offers a comprehensive range of solutions for studying nucleic acid drug molecules, as described below.

Items Types Descriptions
Structural Analysis Primary Structure Analysis NMR can determine the fundamental base sequences of nucleic acid drug molecules, although it is typically more applicable to small molecules or oligonucleotides. High-resolution NMR spectroscopy can reveal the chemical shifts associated with nucleotides in specific chemical environments.
Secondary Structure Analysis NMR can provide valuable information about the local structure of nucleic acid molecules, including the formation of double helices, hairpin structures, and G-quadruplexes. These structures can be identified by several characteristic NMR signals, such as weak internuclear interactions between hydrogen bonds and base stacking.
Three-Dimensional Structure Analysis Complex three-dimensional structures, such as the folding of RNA and DNA or the formation of nucleic acid-protein complexes, can also be elucidated using NMR spectroscopy. Multidimensional NMR experiments provide valuable information on nucleic acid interactions, spatial distances, and torsion angles, which are essential for constructing atomic-level structural models.
Quantitative Analysis - NMR is not only a tool for qualitative analysis, it can also be employed for quantitative analysis. By integrating and analyzing the peak area, the concentration of each component can be accurately quantified. In the context of purity testing, concentration determination, and reaction monitoring during nucleic acid drug development, NMR proves to be an invaluable tool.
Chemical Modification and Labeling Analysis - NMR technology can be utilized to detect and analyze chemical modifications on nucleic acid molecules, including sugar groups, sulfation, and methylation. High-resolution NMR is capable of identifying the changes in chemical shifts caused by these modifications and elucidating their effects on the conformation and function of nucleic acid molecules.
Stability and Degradation Studies - The physical and chemical stability of nucleic acid drugs can be assessed by measuring the changes in chemical shift and peak shape of samples under various conditions (e.g., differing pH levels, temperatures, ionic strengths, etc.) using NMR spectroscopy. Additionally, by monitoring the NMR signals of the degradation products, customers can elucidate the degradation pathways and identify the structures of these products.

Highlights of NMR Technology Platforms

  • This provides structural information at the atomic level, allowing for highly precise resolution of changes and interactions down to the level of individual atoms.
  • Capable of analyzing a diverse array of molecule types, including single-stranded and double-stranded nucleic acids, complex nucleic acid-protein complexes, and more.
  • Dynamic changes in molecules can be monitored in real time, allowing for the assessment of kinetic and thermodynamic parameters.
  • A single experiment can simultaneously gather information on various aspects of structure, interactions, and kinetics, characterized by high efficiency and low cost.

Our Analytical Characterization Services

CD Formulation's NMR technology analysis platform offers comprehensive services, including nucleic acid identification, structural characterization, and impurity analysis, to support genomic research, molecular biology, drug development, and quality control.

Identification for Nucleic Acid Drugs

Our nucleic acid identification service accurately identifies and determines the nucleic acid sequences in samples, helping clients confirm the authenticity and integrity of gene fragments, thereby supporting genomics and molecular biology research.

Structural Characterization for Nucleic Acid Drugs

Our NMR platform provides precise information on bond distances, bond angles and spatial configurations within molecules, helping scientists to understand molecular functions and interaction mechanisms and advancing drug design.

Impurity Analysis for Nucleic Acid Drugs

Our impurity analysis service utilizes NMR technology to comprehensively detect and quantitatively assess impurities in samples, ensuring that the purity and quality meet industry standards. This provides critical support for drug development, quality control, and safety evaluation.

Publication Data

Technology: NMR technology platform for analyzing drugs

Journal: Molecules

IF: 4.2

Published: 2020

Results:

The study of disease modeling at the molecular level is critical for drug development to help improve the treatment and prevention of a wide range of human conditions. Microbial infections remain a major challenge, as pathogens rapidly continue to evolve and develop drug resistance. Cancer cells are also changing genetically and current therapeutic techniques may (or may become) ineffective in many cases. The pathology of many neurological disorders remains a mystery, and the exact etiology and underlying mechanisms remain largely unknown. Nuclear magnetic resonance (NMR) has been playing a crucial role in drug discovery since its introduction decades ago. In just three decades, NMR has become the "gold standard" platform technology for medical and pharmacological research. In this review, the authors will present the main applications of NMR spectroscopy in medical drug discovery and development. The basic concepts, theories and applications of the most commonly used NMR techniques are presented in the text. The authors also summarize the advantages and limitations of the main NMR methods in drug development.

Fig.2 NMR technology platform for analyzing drugsFig.2 NMR analysis of drugs. (Emwas A H, et al., 2020)

By offering precise molecular-level insights, CD Formulation's NMR technology platform significantly supports the development and optimization of nucleic acid drugs, enhancing their effectiveness and stability. Contact us to customize a professional solution tailored to your project.

References

  1. Emwas A H, Szczepski K, Poulson B G, et al. NMR as a "gold standard" method in drug design and discovery. Molecules. 2020, 25(20): 4597.
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