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Ultraviolet-Visible Spectroscopy Platform for Nucleic Acid Drugs

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UV-Vis spectroscopy has become an indispensable tool for the analysis of nucleic acid drugs due to its high sensitivity and broad applicability. CD Formulation's UV-Vis spectroscopy platform offers customers a wealth of information for drug discovery and quality control by measuring the UV and visible absorption characteristics of substances.

Background of UV-Vis Spectroscopy Platform for Nucleic Acid Drugs

Ultraviolet-visible (UV-Vis) technology is a method for the quantitative and qualitative analysis of nucleic acids based on Lambert-Beer's law. This principle states that purine and pyrimidine bases in nucleic acids contain conjugated double bonds, which exhibit a maximum absorption peak at 260 nm when exposed to UV light. This method is widely used due to its ease of operation, minimal equipment requirements, and suitability for determining the concentration of nucleic acids in a sample prior to accurate quantification.

Fig.1 UV-Vis absorption spectroscopy showing DNA derivatives. (Liu H, et al., 2011)

Coupling Technologies for Characterizing Nucleic Acid Drugs

By combining the following technologies, we can analyze nucleic acid drug molecules more comprehensively and extensively, and can gain a deeper understanding of the nucleic acid molecule.

High-Performance Liquid Chromatography (HPLC) Technology
Fluorescence Spectroscopy Technology
Mass Spectrometry (MS) Technology
Circular Dichroism (CD) Technology
Polymerase Chain Reaction (PCR) Technology
Nuclear Magnetic Resonance (NMR) Technology
Electrophoresis Technology
...

Our Solutions of UV-Vis Spectroscopy Platform for Nucleic Acid Drugs

CD Formulation's UV-visible spectroscopy technology platform provides a comprehensive and efficient solution for structural analysis, purity testing, concentration determination and coordination studies of nucleic acid drugs. Our solutions can efficiently assist you in the development of nucleic acid drug formulations.

Purity Analysis of Nucleic Acids

The UV absorption of nucleic acid molecules primarily arises from the conjugated systems present in their bases, particularly adenine, guanine, cytosine, and thymine. These bases typically exhibit a prominent absorption peak at 260 nm. By measuring the position and intensity of this absorption peak, one can determine the purity and concentration of the nucleic acid. If an impurity in the sample interferes with the absorption peak, it may alter the spectrum, enabling the analysis of the sample's purity through the spectrogram.

Concentration Determination of Nucleic Acids

Utilizing the Lambert-Beer law, UV-Vis spectroscopy enables the quantitative determination of nucleic acid concentrations. By measuring absorbance at 260 nm, the actual concentration of the sample can be calculated using either the standard curve method or the dilution method. This process is especially crucial for the quantitative analysis of nucleic acid drugs, as it ensures the consistency and accuracy of drug concentrations.

Nucleic Acid Structure Analysis

UV-Vis spectroscopy can also be used to monitor changes in the molecular structure of nucleic acids. For example, in thermal denaturation experiments, as the temperature increases, the secondary structure of DNA or RNA gradually dissociates, leading to a change in absorbance (a phenomenon known as the hyperchromic effect). By monitoring this change, we can determine the melting temperature (Tm) of nucleic acid molecules and assess their stability.

Ligand and Interaction Analysis of Nucleic Acids

Interactions between nucleic acid drug molecules and target molecules (e.g., proteins, small molecule drugs, etc.) can be monitored using UV-Vis spectroscopy. These interactions frequently induce changes in the absorption spectra, allowing researchers to observe these alterations to analyze the binding properties of nucleic acid drugs with other molecules, including binding constants and the number of binding sites.

Highlights of UV-Vis Spectroscopy Platform for Nucleic Acid Drugs

Our UV-Vis spectroscopy platform is highly sensitive and capable of detecting nucleic acid drug molecules at low concentrations, ensuring accurate and reliable analytical results.
The UV-Vis spectroscopy platform is applicable to various types of nucleic acid drugs, including DNA, RNA, and their fragments, allowing for a comprehensive assessment of the characteristics of these nucleic acid drugs.
The platform allows for the rapid acquisition of spectroscopic data, facilitating efficient sample analysis and conserving valuable research time.
Our platform provides consistent and reproducible results, ensuring the reliability of each measurement and supporting the advancement of long-term research projects.

Publication Data

Technology: UV-visible spectroscopy platform for analyzing DNA

Journal: International Journal of Molecular Sciences

IF: 2.9

Published: 2014

Results:

The authors have extensively studied the attachment of DNA to the surface of laser-generated nanoparticles using dynamic light scattering and UV-visible spectroscopy. The increase in hydrodynamic radius and the performance of UV-Vis spectroscopy highlight the conjugation of DNA. The authors' study demonstrates that dynamic light scattering is a suitable analytical tool to directly and qualitatively demonstrate the conjugation between DNA molecules and gold nanoparticles, and is therefore an ideal tool to monitor changes in the conjugation process when experimental conditions change.

Fig.2 UV-vis spectra of DNA derivatives Fig.2 UV-vis spectra of AuNPs/DNA solution. (Zimbone M et al., 2014)

CD Formulation's UV-Vis spectroscopy technology offers robust support for comprehensive studies of nucleic acid drug molecules. Whether in drug discovery, quality control, or fundamental research, our platforms are specifically designed for these applications. Please feel free to contact us, we are dedicated to providing our customers with exceptional service and technical support to assist researchers in expediting the drug development process.

References

Liu H, Yang L, Ma H, et al. Molecular sensitivity of DNA-Ag-PATP hybrid on optical activity for ultratrace mercury analysis. Chem. Commun. 2011, 47(33): 9360-9362.
Zimbone M, Baeri P, Calcagno L, et al. Dynamic light scattering on bioconjugated laser generated gold nanoparticles. PLoS One. 2014, 9(3): e89048.

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STEP 2

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STEP 3

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