CD Formulation leverages advanced technologies to reveal the complex structures of nucleic acid drugs, aiding the innovation and development of new therapies. Our services provide detailed molecular analyses to ensure each drug performs its intended functions and meets strict research standards.

What is the Higher Order Structure of Nucleic Acid Drugs?

The complex three-dimensional structures of nucleic acid drugs, known as their higher order structures (HOS), are crucial for their biological functionality. These structures extend beyond mere sequences, encompassing intricate tertiary and quaternary formations. Tertiary structures, such as stem-loops, pseudoknots, and G-quadruplexes, result from further spatial folding of nucleic acid chains and are stabilized by various molecular interactions. These configurations guide precise recognition and target binding. Quaternary structures comprise interactions between multiple nucleic acid strands or nucleic acids with proteins, impacting the drug's stability, solubility, and function. Such structures are essential for processes like ribozyme catalysis and the enhanced specificity found in antibody-DNA complexes.

Explore Our Nucleic Acid Drug Higher Order Structure Analysis Services

Tertiary Structure Analysis Services

Our services specialize in examining the 3D shapes of nucleic acids in drug applications. We analyze sequences, secondary structures, and how these nucleic acids interact with proteins or small molecules. Utilizing X-ray crystallography, NMR, and Cryo-EM, we gather detailed structural data. This information aids in understanding how nucleic acids bind specifically to proteins and adapt to environmental changes.

Quaternary Structure Analysis Services

Quaternary structure analysis extends beyond tertiary structure by focusing on dynamic changes and temporal evolution. Molecular dynamics simulations are the core method for studying four-dimensional structures, allowing scientists to observe the movement and changes of nucleic acid molecules at an atomic level. This approach can simulate the true behavior of nucleic acids within a cellular environment and reveal dynamic characteristics difficult to observe directly through experiments.

Structure Prediction and Modeling Services

Structure prediction and modeling employ computer-assisted methods combined with existing experimental structural data to predict unknown three- and four-dimensional structures of nucleic acids. Utilizing machine learning, pattern recognition, and artificial intelligence algorithms, structure prediction tools simulate how nucleic acid sequences fold into their functional structures. This field's advancements facilitate the acquisition of structural information conveniently when experimental methods are limited or resources are scarce, guiding subsequent research.

Workflow of Nucleic Acid Drug Higher Order Structure Analysis Services

Fig.1 Flow chart of nucleic acid purity HOS structure analysis. (CD Formulation)

• Sample Preparation

Extract and purify target nucleic acid fragments to ensure sample integrity and stability, avoiding contamination from external substances.

• Choice of Structural Analysis Method

Select appropriate analytical techniques such as NMR spectroscopy or Cryo-EM based on the characteristics of the nucleic acid and research needs.

• Data Acquisition

Obtain original data from the sample using the selected structural analysis methods, potentially involving complex instrumentation operations and condition optimization.

• Data Processing and Analysis

Process collected data with software tools to perform preliminary structural calculations and analysis, resulting in a three-dimensional structural model of the nucleic acid.

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Our Advantages of Nucleic Acid Drug Higher Order Structure Analysis

• Higher order structure analysis provides more detailed molecular structure information, enhancing analysis accuracy and reliability, supporting in-depth scientific research exploration.
• Nucleic acid drugs' higher order structure can be used to study stability under different environmental conditions, aiding in the optimization of storage and transportation conditions.
• Analyzing higher order structures helps understand nucleic acid drug interactions with target molecules, promoting drug design and development.
• During production, higher order structure analysis is used for quality control, ensuring product consistency and standardization.
• This analysis can reveal potential variations or adverse factors, ensuring drug safety.

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To learn more about our higher order structure analysis services for nucleic acid drugs, or for specific technical discussions, please contact our professional team. We're ready to provide customized service solutions to meet your specific needs.