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Custom Labeled & Conjugated Peptide Synthesis

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Peptides, those chains of amino acids, can be adorned or bound through a multitude of innovative techniques, dictated largely by their unique sequences and the specific research endeavors they serve. At CD Formulation, our adept team stands ready to deliver bespoke synthesis services, crafting custom-labeled and conjugated peptides of the highest caliber for a clientele that spans the globe.

About Labeled & Conjugated Peptide Synthesis

Peptide tags and the myriad techniques for peptide blinking in the realm of peptide synthesis present possibilities. Among these, the most frequent labeling includes N-terminal labeling, N-terminal fluorescein modification, photosensitizer alteration, C-terminal labeling, isotope labeling, and imaging agent labeling. At the forefront of biochemical explorations are fluorescent peptides and their labeled counterparts. Moreover, the process of conjugating peptides with a diverse array of molecules opens the gateway to enhancing stability, fine-tuning immunogenic responses, and sharpening targeting precision. This fusion of peptides can yield a cornucopia of innovative research tools, including:

Peptide enzyme conjugates for diagnostic analysis.
Peptide antibody conjugates for drug therapy.
Peptide-polyethylenimine (PEI) conjugates for in vivo imaging and DNA delivery.
Biotin-peptide conjugates for immunoassays.
Peptide antibody conjugates for immunoassays.
DNA, RNA-peptide conjugates for studying protein-protein interactions.
siRNA-peptide conjugates for siRNA delivery.
Peptide-agarose complexes for affinity chromatography immobilization.
Peptide-drug conjugates for drug delivery.
Peptide-lipid complexes for cellular uptake.

Fig. 1 Peptide-drug conjugates Fig. 1 Schematic diagram of the components of peptide-drug conjugates. (Chavda VP, et al., 2022)

Explore Our Custom Labeled & Conjugated Peptide Synthesis Services

CD Formulation thrives in the intricate world of peptide synthesis, expertly crafting an array of labeled and conjugated peptides. Our synthesis protocols, robust and reliable, intermingle with cutting-edge peptide analysis technologies, empowering us to unravel even the most daunting challenges that stumble across the path of peptide therapeutic development and production.

Undoubtedly, we wield the finest synthesis technologies, meticulously orchestrating the peptide synthesis process to guarantee not just efficiency but also safety and stability—elements that intertwine with the varying complexities of each unique project. We aim to elevate our customers' experiences by delivering peptides of unparalleled quality, enabling them to seamlessly navigate the labyrinth of drug development.

The following are high-quality labeled and conjugated peptide options that CD Formulation can provide:

Peptide Labeling & Conjugation
Enzyme Labeled Peptide	Isotope Labeled Peptide	Imaging Agent Labeled Peptide
Quenched Fluorescent Peptide (FRET Peptide)	Fluorescence Labeled Peptide	Carrier Protein-Conjugated Peptide
DNA-Conjugated Peptide	RNA-Conjugated Peptide	siRNA-Conjugated Peptide
Nanoparticle-Conjugated Peptide	Gold Nanoparticle-Conjugated Peptide

Labeled & Conjugated Peptide Purification Methods

Although most peptide synthesis methods are highly efficient, many of these processes can still generate unwanted impurities due to incomplete deprotection, unwanted reactions between free-protecting groups, truncation and/or deletion of amino acids, isomers, and other byproducts.

Our scientists can repeatedly remove these impurities by using one or more of the following cleanup techniques:

Peptide Manufacturing & Analytical Services

In addition to peptide synthesis capabilities, we combine flexible GMP manufacturing facilities with cutting-edge peptide analytical knowledge to provide a full range of quality control testing services to accelerate the commercialization of your products, including:

Peptide identification (ESI-MS).
Peptide Molecular weight determination.
Peptide sequencing.
Peptide quantification/peptide content determination.
Peptide purity and impurity analysis (HPLC/UV).
Amino acid sequence.
Amino acid composition determination.
Net peptide content.

Enantiomeric purity testing (GC/MS; LC).
Residual counterion testing (e.g. TFA).
Elemental analysis.
Residual solvent testing.
Water content testing (GC or KF).
Peptide solubility testing.
Peptide stability testing.

Optical rotation determination.
Bioburden testing(TAMC/TYMC).
Bacterial endotoxin testing.
Sterility testing.
Cytotoxicity testing.
Process/product related impurity testing.
Other pharmacopoeia testing.

Why Choose Us for Custom Labeled & Conjugated Peptide Synthesis?

Flexible technology portfolio, including solid phase, liquid phase, and microwave technology.
Our peptide synthesis success rate is greater than 95%.
We provide a full range of peptide labeling and conjugation services: fluorescent dyes, non-fluorescent dyes, isotopes, imaging agents, DNA, RNA...
Our expert team is proficient in hundreds of cross-linking modifications, activation,s and conjugation reactive cross-linkers, and optimizes labeling and conjugation rates according to customer requirements.
All our samples are carefully stability monitored and characterized to ensure batch-to-batch consistency.

Publication

Published Data

Technology: Peptide-Drug Conjugates Synthesis

Journal: Pharmaceutics.

IF: 4.93

Published: 2023

Results:

The authors designed and optimized a synthetic method for JH-VII-139-1 and its c(RGDyK) peptide conjugates. JH-VII-139-1 is an analog of Alectinib linked to the α ν β 3 targeting oligopeptide c(RGDyK) via amide, carbamate, and urea linkers. The chemical stability, cytotoxicity, and antiangiogenic properties of the synthesized hybrids were investigated. Cytotoxicity results showed that all conjugates retained mid-nanomolar SRPK1 kinase inhibitory activity, and two of the four conjugates, geo75 and geo77, exhibited low micromolar IC 50 values of antiproliferative effects against HeLa, K562, MDA-MB231, and MCF7 cancer cells. In vivo zebrafish screening assays showed that the synthesized conjugates were active against intersegmental vessel (ISV) length or width. These data indicated the c(RGDyK) peptide conjugates demonstrated similar potency to JH-VII-139-1 in inhibiting SRPK1 and in some ways inhibited cell and vessel growth.

Fig. 2 JH-VII-139-1 and c(RGDyK) peptide conjugates. Fig. 2 General structures of alectinib, JH-VII-139-1 and c(RGDyK) peptide conjugates that carry an SRPK1 inhibitor. (Leonidis G, et al., 2023)

CD Formulation has extensive experience in custom labeled and conjugated peptide synthesis. Each synthesis step is subject to our strict quality control. Please don't hesitate to contact us if you would like to learn more about the possibilities of your peptide modification. We look forward to cooperating with you.

References

Ahrens VM, Bellmann-Sickert K, Beck-Sickinger AG. Peptides and peptide conjugates: therapeutics on the upward path. Future Med Chem. 2012 Aug;4(12):1567-86.
Chavda VP, Solanki HK, Davidson M, et al. Peptide-Drug Conjugates: A New Hope for Cancer Management. Molecules. 2022; 27(21):7232.
Leonidis G, Koukiali A, Sigala I, et al. Synthesis and Anti-Angiogenic Activity of Novel c(RGDyK) Peptide-Based JH-VII-139-1 Conjugates. Pharmaceutics. 2023 Jan 22;15(2):381.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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