CD Formulation specializes in quant analysis for nucleic acid drugs, offering precise and efficient support to research bodies and businesses. Our lab, armed with cutting-edge tools and a skilled team, caters to diverse quant analysis requirements in nucleic acid drug research.

About Quantitative Analysis of Nucleic Acid Drugs

Nucleic acid quantification is prevalent in many workflows across various application fields. These detection methods use absorbance or fluorescence to measure the concentration of nucleic acids in a sample. Many laboratories have adapted single cuvette schemes to high-throughput formats like 96-well and 384-well microplates, and more recently to micro-volume containers. These standardized detection forms, along with instruments capable of recording measurement results, can quickly quantify a large number of samples. Additionally, measurements taken at multiple wavelengths (260 nm and 280 nm) or UV-Vis scans can determine the purity of samples to identify contamination from chemical additives used during separation.

Explore Our Nucleic Acid Drug Quantitative Analysis Services

DNA quantification and RNA quantification are generally referred to as nucleic acid quantification, which typically involves measuring the average concentration of DNA or RNA in a sample before downstream experiments. Two commonly used optical techniques for nucleic acid quantification are UV-visible measurement and fluorescence measurement. Choosing the appropriate technique for your samples, workflows, and throughput can enable accurate quantification of RNA or DNA, preventing downstream experiment failures and saving significant time and money.

UV Spectrophotometric Analysis of Nucleic Acids

This technique measures how nucleic acids like DNA and RNA absorb UV light, typically at 260 nm. As the sample absorbs more UV light, indicating higher nucleic acid concentration, less light reaches the detector, resulting in increased optical density (OD).

Fluorescence Analysis of Nucleic Acids

This sensitive method uses fluorescent dyes to bind nucleic acids, allowing detection and quantification of specific molecules. The emitted fluorescence correlates linearly with nucleic acid concentration, offering precise quantification. Its high sensitivity and specificity make it invaluable in various labs for nucleic acid analysis.

Our Solutions of Nucleic Acid Drug Quantitative Analysis Services

Analyzing nucleic acid drugs demands combining cutting-edge bioanalysis techniques and novel experimental methods for precise measurement in complex matrices. This involves using advanced chromatography, mass spectrometry, sensitive detection tools, and data algorithms, ensuring accurate and repeatable outcomes. Moreover, diverse sample preparation strategies and methodological standardization are considered to adapt to different research environments and needs. Such integrated solutions facilitate deeper research, supporting new drug development and product quality monitoring.

Our Workflow of Nucleic Acid Drug Quantitative Analysis Services

Fig.1 Flow chart of nucleic acid drug quantitative analysis. (CD Formulation)

• Sample Preparation

Sample preparation involves the extraction of nucleic acids from biological samples, which serves as the foundation for subsequent quantitative analysis.

• Nucleic Acid Purification

Chemical reagents and column purification techniques are employed to eliminate impurities and enhance the purity of nucleic acids, thereby ensuring analytical accuracy.

• Quantitative Detection

Quantitative detection employs advanced techniques such as fluorescence quantitative PCR, digital PCR, or next-generation sequencing to perform precise quantitative analysis of nucleic acids.

• Data Processing

Professional software is utilized to organize and analyze the data collected, ensuring reliable results.

• Report Generation

According to the analyzed data, please write a detailed analysis report that includes the experimental methods, results, and conclusions.

Our Technology Platforms

Nucleic acid drug quantitative analysis requires the use of numerous advanced technology platforms, including but not limited to HPLC, LC-MS, NMR, fluorescence qPCR, SPR, and other technologies.

Our Advantages of Nucleic Acid Drug Quantitative Analysis Services

• Our services deliver precise and sensitive nucleic acid quantification, accurately identifying nucleic acid levels in samples.
• Our service excels in rapid analysis, swiftly assessing complex samples and enhancing research and development efficiency.
• We ensure consistent test outcomes, emphasizing repeatability and reliability.
• With cutting-edge technology, our service handles diverse sample types, expanding nucleic acid analysis possibilities.

Publication Data

To learn more about our specialized services in nucleic acid quantification, feel free to contact our team at CD Formulation. Our specialists are available and eager to support you. We are excited about the possibility of working together!