Technologies & Platforms

Start Your Project Now!

Please note: Our products and services are not intended to be used directly in diagnostic or therapeutic procedures.

Here's how you can reach us...

Tel:
Email:

Real-Time Quantitative PCR (qPCR) Technology Platform for Nucleic Acid Drugs

Inquiry

At CD Formulation, we offer state-of-the-art qPCR assays to our valued customers. With qPCR, we provide accurate and reliable quantitative analysis of nucleic acid targets, ensuring unparalleled sensitivity and specificity.

About Real-Time qPCR for Nucleic Acid Drugs

Real-time quantitative PCR (qPCR) involves the incorporation of fluorescent groups, such as fluorescent dyes or fluorescently labeled specific probes, that selectively bind to DNA products within the PCR reaction system. This labeling allows for the tracking of PCR products throughout the reaction. As the PCR process progresses, the reaction products accumulate, leading to a proportional increase in the intensity of the fluorescent signals. After each cycle, the fluorescence intensity is measured, enabling real-time monitoring of product quantity based on changes in fluorescence signal intensity. By utilizing corresponding software to analyze the data, a fluorescence amplification curve can be generated. This curve facilitates the calculation of the target gene quantities in the initial template of the samples being tested, making qPCR a valuable tool for assessing changes in the expression levels of target genes.

Fig.1 Advantages of real-time quantitative PCR (qPCR) Fig.1 Characteristics of Real-Time qPCR. (CD Formulation)

Capabilities of Our Real-Time qPCR Platform

CD Formulation offers robust support for basic research, medical diagnostics, and biopharmaceutical development through a real-time fluorescent quantitative PCR technology platform that efficiently and accurately quantifies various types of nucleic acids.

DNA Analysis

Real-time fluorescent quantitative PCR technology can accurately quantify and analyze genomic DNA (gDNA). This technique detects specific DNA sequences and provides information about their relative or absolute quantities.

Complementary DNA (cDNA) Analysis

By converting RNA into complementary DNA (cDNA) through reverse transcription, Real-Time Fluorescent Quantitative PCR can analyze and quantify cDNA.

mRNA Analysis

Real-time fluorescent quantitative PCR technology can be utilized to quantify messenger RNA (mRNA) in cells or tissues. The detection and quantification of mRNA levels serve as essential tools for investigating the regulation of gene expression, cell differentiation, and pathological mechanisms.

Long-stranded Non-coding RNA (lncRNA) Analysis

Real-time fluorescence quantitative PCR technology can detect and quantify long-chain non-coding RNA (lncRNA), which are RNA molecules that play a crucial role in regulating gene expression and epigenetic modifications.

Highlights of Our Real-time qPCR Platform for Nucleic Acid Drugs

Comprehensive coverage of assays, including various protocols for different types of nucleic acids.
We specialize in primer design and can create high-quality primers tailored to our customers' specific needs and RNA types.
Imported high-efficiency dyes and quantitative instruments yield accurate and reliable results.
Reliable reporting of test results and the ability to create publishable graphs tailored to customer needs.

Our Analytical Characterization Services

CD Formulation provides a real-time fluorescent PCR platform, an advanced tool for nucleic acid quantification and impurity analysis. This platform is dedicated to the development of nucleic acid preparations and bioanalytical services.

Quantitative Analysis of Nucleic Acid Drugs

Our platform facilitates precise quantitative analysis of DNA and RNA samples, effectively supporting the development and quality control of various nucleic acid formulations.

Impurity Analysis for Nucleic Acid Drugs

For the analysis of nucleic acid impurities, real-time qPCR is highly effective for identifying PCR inhibitors and non-specific amplifications.

Publication Data

Technology: Real-time qPCR technology for accurate diagnostic testing

Journal: Biosensors

IF: 4.9

Published: 2023

Results:

Nucleic acid amplification technologies (NAATs) can be used for rapid and accurate diagnostic testing, providing critical information for better follow-up and prognosis. Nucleic acid amplification techniques are widely used because they are characterized by high sensitivity, high specificity, and fast amplification and detection. It is worth noting that different NAATs can be selected according to different environments and research areas, for example, developing countries or resource-poor areas can prioritize isothermal amplification methods with simple operation. In the field of translational medicine, CRISPR has shown great promise. the core component of NAAT is the activity of different enzymes. As the most critical material for nucleic acid amplification, the key role of enzymes is self-evident and plays the most important role in molecular diagnostics.

Fig.2 Real-time qPCR technology for accurate diagnostic testing Fig.2 PCR comparisons: end-point PCR, qPCR and dPCR.

CD Formulation, a company with extensive experience in biological sample analysis and assays, offers a comprehensive range of analytical services for nucleic acid drug development and quality control. Our services include real-time qPCR technology, which quantifies the presence of specific nucleic acid targets in samples. Contact us, and we will customize an exclusive experimental protocol tailored to your project.

References

Wang M, Liu H, Ren J, et al. Enzyme-assisted nucleic acid amplification in molecular diagnosis: a review. Biosensors. 2023, 13(2): 160.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

Related Services