Surface Plasmon Resonance (SPR) Platform for Nucleic Acid Drugs

Surface Plasmon Resonance (SPR) is a highly effective analytical technique for the label-free detection of a diverse array of analytes, as well as for real-time monitoring of biomolecular events. This includes the assessment of binding kinetics, affinity, and specificity of interactions between biomolecules, such as nucleic acids. CD Formulation provides an SPR technology platform that facilitates high-throughput screening of fragment libraries, determination of kinetics and affinity, analysis of binding specificity, and characterization of antibodies.

Principles of Surface Plasmon Resonance (SPR) Technology

Surface Plasmon Resonance (SPR) technology is based on surface plasma waves generated when light interacts with a metal surface. When the frequency of the incident light resonates with the free electrons on the metal surface, a surface plasma wave is formed. The propagation of this wave alters the intensity of the incident light, resulting in a change in the intensity of the reflected light. When biomolecules bind to the metal surface, they modify the local refractive index, which in turn affects the propagation of the surface plasma wave, leading to a change in the intensity of the reflected light.

The advantages of our SPR technology include:

• High Sensitivity
• High Throughput
• Quantitative Analysis
• Versatility
• ...

Fig.1 Advantages of SPR Technology Platform. (CD Formulation)

Our Applications of SPR Platform for Nucleic Acid Drugs

Workflow of Our SPR Technology Platform for Nucleic Acid Drugs

Fig.2 Workflow of SPR technology platform. (CD Formulation)

Sample Preparation

Customers are required to provide the molecules for testing, including nucleic acids, potential ligands, or small molecule drugs.

Chip Surface Modification

We will implement suitable chemical modifications to the chip surface of the SPR instrument to ensure that the molecules being tested can be stably immobilized on the chip.

Experimental Design

We design the experimental program based on the customer's requirements, including sample concentration, flow rate, temperature, and other parameters.

Data Acquisition

Conduct experiments using the Surface Plasmon Resonance (SPR) instrument, monitor changes in reflected light intensity in real time, and document binding events.

Data Analysis

Analyze the collected data to calculate binding constants, dissociation constants, kinetic parameters, and other relevant metrics.

Report Generation

We will provide a comprehensive experimental report that includes the experimental conditions, data analysis results, and conclusions.

Highlights of Our SPR Technology Platform for Nucleic Acid Drugs

• SPR technology facilitates real-time monitoring of molecular binding without the necessity for labeling or immobilizing the sample.
• SPR technology is highly sensitive to changes in refractive index caused by molecular binding and can detect even minor variations.
• Compared to traditional labeling techniques, SPR technology does not require chemical modification or labeling of the sample.
• SPR technology can be utilized to investigate protein-protein, protein-nucleic acid, protein-small molecule, and various other types of intermolecular interactions.

Our Analytical Characterization Services

CD Formulation's SPR technology platform offers a comprehensive array of services for analyzing nucleic acid preparations. Our platform enables us to design experimental protocols tailored to customer requirements, collect data in real time using SPR instruments, and monitor binding events for thorough data analysis.

Publication Data

CD Formulation offers SPR technology platforms designed to analyze interactions between biomolecules, as well as the kinetic properties of molecules, affinity binding rates, and dissociation rates. We provide a range of assays, including but not limited to protein-DNA and DNA-DNA interactions. Contact us for a customized solution tailored to your project.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.