Peptide-ASO Conjugate Development

CD Formulation's services in developing Peptide-ASO Conjugates aim to assist researchers and organizations in crafting versatile biomolecules. By merging peptides with antisense oligonucleotides, this tech expands the horizon for scientific inquiry and novel implementations.

Background of Peptide-ASO Conjugate Development

The creation of peptide-ASO conjugates focuses on leveraging the benefits of peptides and ASOs to improve precision in targeting and efficiency in cellular delivery. This involves chemically joining these two components to capitalize on peptides' targeting abilities and ASOs' gene regulation capability, thus improving their effectiveness in reaching specific targets. Early research focused on optimizing conjugation methods and ensuring stability to address challenges related to membrane penetration and specific recognition.

Explore Our Services for Peptide-ASO Conjugate Development

Development of Peptide-ASO Conjugate Linking Methods

In crafting peptide-ASO Complexes, peptides and oligos are automatically synthesized on solid supports. This enables the formation of robust links after synthesis using diverse chemical reactions such as oxime, amide, carbonyl, thioether, disulfide bonds, and click techniques. This flexible coupling method not only increases the success rate of synthesis but also enhances the functional properties of the final conjugate, laying a foundation for future applications.

Development of Binding Sites for Peptide-ASO Conjugates

When forming peptide-ASO conjugates, the peptide's N- or C-terminus often connects to the oligonucleotide's 5' or 3' end. This strategy aids in chemical linkage without significantly affecting base pairing. Beyond terminal sites, the 2' position of ribose, internal nucleobases, and the phosphodiester bond between nucleotides can also serve as effective binding sites. This variety in binding options provides flexibility in optimizing the performance of the conjugate, ensuring the final product's functionality and stability.

Our Workflow of Peptide-ASO Conjugate Development

Fig.1 Flow chart of peptide-ASO conjugate development. (CD Formulation)

• Requirements Analysis and Design

Define the project's goals, design the appropriate peptide-ASO conjugate structure, and select suitable peptide segments and oligonucleotides.

• Synthesis and Modification

Individually synthesize peptides and oligonucleotides, and perform necessary chemical modifications to optimize their performance.

• Conjugation Reaction

Connect peptides and oligonucleotides by selecting suitable chemical bonds (such as oxime or amide bonds) to ensure the stability of the conjugation.

• Performance Evaluation

Conduct tests on the peptide-ASO conjugates for stability, affinity, and cellular uptake to assess overall performance.

• Quality Control and Delivery

Implement strict quality control to ensure each conjugate meets the standards, and compile relevant documentation for client reference.

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Advantages of Peptide-ASO Conjugate Development Services

• We specialize in designing tailored peptide-ASO structures, selecting optimal peptide sequences and oligonucleotides to best suit your project needs.
• Utilizing a blend of automated and manual synthesis methods, we ensure precise construction and modification of peptide and oligonucleotide components for optimal performance.
• Our capability in forming stable linkages, like oxime and amide bonds, guarantees effective and stable conjugate formation.
• Detailed assessments of stability, affinity, and cellular uptake help ascertain the overall efficacy and suitability of conjugates.
• Stringent quality checks are integrated throughout the process, ensuring every conjugate meets high standards before delivery, accompanied by full documentation for client reference.

Published Data

CD Formulation is dedicated to providing clients with high-quality Peptide-ASO Conjugates development services. Please feel free to contact us for more information about our services.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.