HET-CAM Test

The HET-CAM test is suitable for the screening and testing of cosmetics, cosmetic ingredients/cosmetic eye irritation/corrosive effects. CD Formulation provides tests that help the safety laboratory of the R&D center to carry out the screening and safety evaluation of formulations/raw materials, to be able to distinguish possible non-irritants or mild irritants, and to better rank risky substances.

Testing Background

The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. Since the surface of the chick embryo allantoic membrane is rich in blood vessels and can be considered as an intact organism, this test takes advantage of the intact, clear and transparent vascular system of the chick embryo in the middle stage of hatching. This is used to assess the ocular irritation of the test substance.

Our Services

CD Formulation has laboratories for physicochemical testing, microbiological testing, efficacy assessment, and irritation assessment. We can provide our clients with HET-CAM chicken embryo allantoic membrane testing services to establish irritation assessment methods for chicken embryo allantoic membrane vessels, quantify the irritation of raw materials and products to ensure the development of safe and low irritation products, and make timely data analysis for irritation and provide real test data.

Test Procedure

• CAM preparation

The 9-day-old chicken embryo was removed from the top of the air chamber, the egg shell was removed, an appropriate amount of saline was added to the egg membrane, the egg membrane was carefully peeled off with medical curved forceps to expose the allantoic membrane, and the integrity of the allantoic membrane was observed.

• Cosmetic preparation

Regardless of the sample properties, the product can be tested using either the original sample or the target concentration. If liquid, apply 0.3 mL to the CAM. if solid, grind to a fine powder or flake, then moisten with a small amount of pure water and weigh 0.3 g and apply to the CAM. if the sample is a paste, apply the subject to the surface of a plastic film, flip the film over, and bring the sample surface into contact with the CAM.

• Positive/negative control

Before conducting the formal test, a negative control test and a positive control test are performed. The negative control is a 0.9% sodium chloride solution; the positive control is a substance capable of producing a severe reaction.

• Observation of subjects and calculation of scores

For the formal test, samples were aspirated or weighed on the allantoic membrane, covering the surface as much as possible, and immediately after a certain period of exposure, the degree of change of each toxic effect was observed under a stereomicroscope, and the time or degree of occurrence of each bleeding, clotting, and vascular melting from the beginning of the test was observed and recorded for irritation scoring, using the time assessment method and the endpoint scoring method.

If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.