SDS-PAGE Technology

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the most commonly used analytical technique in protein characterization, can qualitatively analyze protein molecular weight and quantitatively analyze protein aggregates, providing key information about protein purity, molecular weight, and homogeneity. CD Formulation integrates cutting-edge SDS-PAGE technology into our protein characterization technology platform to provide reliable analytical support for the development and characterization of your protein/peptide drugs, from early development to late product release testing.

What is SDS-PAGE Technology?

SDS-PAGE is a widely used laboratory technique for separating and analyzing proteins based on their size. This technique is a basic electrophoresis technique that can separate proteins based on the differences in charge and mobility of different molecular sizes. If the protein sample is highly purified and contains only one protein, the result will be a single protein band after SDS-PAGE separation. When the protein sample contains multiple proteins, the different proteins will be separated into multiple protein bands after SDS-PAGE separation. Therefore, SDS-PAGE technology provides an intuitive method for analyzing the purity of protein samples.

SDS-PAGE Principle

SDS-PAGE is based on the principle that charged molecules migrate towards an electrode of opposite charge under the influence of an electric field. SDS, an anionic detergent, is used to denature proteins and impart a uniform negative charge to them. Since the bound SDS/protein (w/w) ratio is practically independent of the protein, SDS-bound proteins are able to migrate through the polyacrylamide gel matrix when an electric field is applied. The migration speed depends primarily on their size, allowing precise separation and estimation of molecular weight. In addition, information on the nature of covalent bonds (disulfide-mediated or non-disulfide-mediated) can be obtained by comparing the electropherograms of proteins analyzed under reducing and non-reducing conditions.

Our Services Related to SDS-PAGE Technology

Thanks to decades of experience in supporting protein/peptide biopharmaceutical development and manufacturing using SDS-PAGE technology, our team of highly qualified experts offers a range of SDS-PAGE-related services to accelerate the implementation and success of your projects.

Our experienced team of experts has completed hundreds of protein and peptide SDS-PAGE analysis projects, allowing support for all stages of your protein/peptide drug development and manufacturing - from early studies to downstream process monitoring and GMP batch release testing.

Utilizing cutting-edge SDS-PAGE technology, we support the following protein/peptide characterization plans, including but not limited to:

Protein Purity Analysis (Relative Quantitation)

SDS-PAGE is an important tool for assessing protein sample purity. By comparing the intensity and pattern of the protein bands to known standards, our scientists can detect the presence of contaminants or degradation products.

Protein Molecular Weight (MW) Measurement

SDS-PAGE provides a highly accurate method for protein molecular weight determination, which is critical for protein identification and validation. This application is especially valuable in confirming the expression of recombinant proteins and analyzing post-translational modifications that may alter protein quality.

Protein Aggregation Characterization

The presence of variants such as isomers, oligomers or degradation products can seriously affect the stability and function of the protein. Our scientists use SDS-PAGE technology to visualize protein bands to monitor and assess these variants.

Post-Translational Modifications (PTMs) Analysis

SDS-PAGE can be used for comparative protein analysis. Our scientists use SDS-PAGE to identify changes in post-translational modifications and to study protein-protein interactions between different samples.

Our Procedure for SDS-PAGE Analysis

Fig. 1 Workflow for SDS-PAGE analysis. (CD Formulation)

• Sample preparation: Add 2x loading buffer (5% β-ME) and boil the sample for 10 minutes.
• Electrophoresis preparation: Preparation of gel and preparation of electrophoresis buffer.
• Protein sample loading: Take an appropriate amount of processed protein sample according to the protein concentration and add it to the pocket of the gel. After all samples are loaded, add protein standard markers to the first or last pocket of the gel.
• Electrophoresis: Turn on the power and run the gel.
• Gel staining: After the electrophoresis separation is completed, stain with a suitable stain for 1 hour, and then decolorize the protein gel until the background is clean and clear.
• Purity and molecular weight analysis of protein samples: Use image processing software to analyze the gel image and calculate the relative intensity of each protein band to obtain key information about molecular weight and purity.

Advantages of Our SDS-PAGE

• High sensitivity, allowing identification of protein and peptide samples with extremely low concentrations.
• High throughput, allowing identification of dozens to hundreds of proteins at a time.
• High reproducibility, suitable for quantitative comparison between protein samples.
• Separation based on molecular size, allowing separation of complex protein mixtures.
• Compatibility with protein blotting, which can be easy for quantifying specific proteins.
• Automated operation with LC and MS, fast analysis speed, and good separation effect.
• Visual analysis that staining methods (such as Coomassie Brilliant Blue or Silver Staining) can visualize proteins in the gel, making it easy to analyze and record.
• Simple samples, easy detection.

Custom SDS-PAGE Services

Why Choose Our SDS-PAGE Technology?

• Our team consists of well-trained professionals with extensive experience in protein analysis, ensuring reliable and reproducible results.
• We have a team of experts with rich experience in SDS-PAGE analytical method development and validation.
• We have accumulated decades of expertise and successful project experience using SDS-PAGE technology to support protein/peptide biopharmaceutical development.
• We use best-in-class reagents and state-of-the-art equipment for SDS-PAGE, maximizing the accuracy and quality of your protein separations.
• We also offer consulting services to help you optimize your SDS-PAGE protocols and provide support throughout the process.
• We prioritize efficiency without compromising quality, offering fast turnaround times to meet your project deadlines.
• We provide flexible experimental design and customized solutions.

Publication

CD Formulation aims to provide a powerful analytical tool for the separation, purification, and characterization of proteins and peptides. Please feel free to contact us if you are interested in our services. Learn how our SDS-PAGE technology can support the smooth implementation of your protein/peptide biopharmaceutical program.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.