CD Formulation provides top-notch ribose modification services to improve the binding affinity and nuclease resistance of nucleic acids. Our extensive services target the limitations of unmodified nucleic acid therapeutics, supporting their use in precision medicine.

Overview of Ribose Modification

Chemical modifications of the furanose fraction, specifically deoxyribose in DNA and ribose in RNA, have been the subject of extensive research aimed at enhancing binding affinity, reducing susceptibility to nuclease cleavage, improving the cellular distribution of oligonucleotides, and modulating their activity. Furthermore, numerous modifications exploit electronic interactions to preferentially align sugar conformations, thereby influencing the thermodynamic stability of the nucleic acid chain. Ribose can be modified with various functional groups, including 2'-O-methyl (2'-OMe), 2'-O-methoxyethyl (2'-O-MOE), 2'-fluoro (2'-F), azide, and alkynyl groups.

Fig.1 Ribose chemical modifications. (Martinelli D D., 2024)

Explore Our Ribose Modification Services

CD Formulation's comprehensive modification and bioconjugation services assist clients in producing nucleic acids with enhanced potency, bioavailability, stability, reduced toxicity, and minimized off-target effects.

Azide Ribose Modification Services

The modification of ribose through the incorporation of azides typically entails the cyclization of the azide group with the alkyne group present on the ribose, resulting in the formation of a stable cyclic compound. This reaction facilitates selective modifications within the RNA molecule. Initially, nucleotide precursors containing azido groups are synthesized. Subsequently, the desired target molecules are prepared through chemical synthesis, transcription, or cellular extraction, depending on the specific experimental design and requirements. The synthesized azido-modified nucleosides are then combined with the target ribose molecules and subjected to appropriate reaction conditions. Finally, the resulting modification products are purified, and their structures and purity are confirmed using mass spectrometry analysis, Nuclear Magnetic Resonance (NMR), and other analytical techniques.

Alkynyl Ribose Modification Services

Alkynyl Ribose Modification Service is a chemical modification technique employed in nucleic acid molecules. This process involves the introduction of an alkyne group, characterized by a carbon-carbon triple bond functional group, into the ribose structure. Such modifications can create a reactive site for subsequent bioorthogonal reactions and additional chemical alterations.

General Workflow for Ribose Modification

Fig.2 Flow chart of ribose modification. (CD Formulation)

• Design and Optimization

In accordance with the specified requirements, we develop an appropriate modification scheme and implement the necessary optimization measures to ensure both the stability and efficacy of the modification.

• Synthesis and Modification

Ribose is produced and chemically altered through various chemical synthesis technologies.

• Quality Control

The assessment of the purity of modified ribose and the efficiency of the modification typically involves analytical techniques such as MS and NMR spectroscopy to confirm the accuracy of the modification.

• Product Delivery

The completed modified ribose will be meticulously packaged and delivered to the customer, accompanied by pertinent experimental data and usage instructions.

Our Technology Platforms

Solid Phase Synthesis Technology Platform

The synthesis of modified nucleic acids can be achieved through solid-phase synthesis technologies, which are frequently employed in the production of nucleic acids.

MS Platform

This platform is used to determine the molecular weight and structure of modified nucleic acids.

HPLC Platform

This platform is used for purification and quantitative analysis of modified nucleic acids.

NMR Spectroscopy Platform

The platform is used to analyze the chemical structure and modification sites of nucleic acids.

Why Choose Us for Ribose Modification Services?

• Our ribose modification technology is typically distinguished by its high modification efficiency and rapid reaction kinetics.
• The various types of ribose modifications that we provide are compatible with a diverse array of reagents and reaction conditions, thereby broadening their applicability across multiple fields.
• Our ribose modification technology is applicable not only to the investigation of RNA structure, function, and interactions, but also to the fabrication of biomaterials with specific functionalities, as well as to the design and development of pharmaceutical compounds, among other domains.

Published Data

CD Formulation offers high-quality ribose modification services tailored to meet your specific requirements. If you require more detailed information regarding our services, please do not hesitate to contact us.