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Molecular Weight Analysis for Nucleic Acid Drugs

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CD Formulation offers specialized services in analyzing the molecular weight of nucleic acid drugs, like siRNA, to help researchers better comprehend these essential molecules. These drugs consist of long nucleotide chains, possessing molecular weights between a few to several dozen kDa. Accurate molecular weight analysis provides vital data that supports the enhancement of nucleic acid drug performance.

About the Analysis of Molecular Weight for Nucleic Acid Drugs

Molecular weight analysis is crucial for nucleic acid drugs as it dictates their stability and pharmacokinetic profile within the body. Accurate measurement aids in understanding these drugs' mechanisms and supplies vital data for their development and refinement. Discrepancies in molecular weight can impact efficacy and activity, making thorough analysis essential. This not only helps ensure product quality but also supports informed scientific research and development decisions.

Nucleic Acid Drugs	Length	Molecular Weight
ASOs	8-50 bases	5-10 kDa
siRNA	21-23 bases	13-15 kDa
miRNA	20-25 bases	18-23 kDa
mRNA	Range from tens to thousands of base pairs	20-40 kDa
Aptamers	50 to 120 bases	Based on their sequence length

Explore Our Services for Nucleic Acid Drug Molecular Weight Analysis

Items	Descriptions
Pretreatment of Nucleic Acid Drugs	Preparing these drugs for analysis is tricky since nucleic acids degrade easily and bind with biomolecules. Techniques like liquid-liquid and solid-phase extraction are used to boost nucleic acid recovery and reduce protein and impurity interference.
Molecular Weight Analysis	Typically, HPLC with mass spectrometry (LC-MS/MS) is used to precisely measure nucleic acid drug molecular weights and oligomer distributions. This approach also enables the study of single and double-stranded structures.
Mass Spectrometry Optimization	Mass spectrometry signals can suffer due to ion distribution issues and cation interference. Addressing this may involve tweaking the ESI source, adding volatile cations, and optimizing chromatographic conditions.

Our Workflow of Nucleic Acid Drug Molecular Weight Analysis Services

Fig.1 Workflow of nucleic acid molecular weight analysis. Fig.1 Flow chart of nucleic acid molecular weight analysis. (CD Formulation)

Sample Preparation

Remove proteins and contaminants from nucleic acid drug samples to ensure they don't affect analytical results.

Quality Control

Perform initial quality checks on samples to confirm they meet analytical standards, focusing on concentration and purity.

Selection of Analytical Methods

Choose the best analytical technique, such as mass spectrometry or gel electrophoresis, based on the nucleic acid drug's characteristics.

Data Collection and Analysis

Gather data on nucleic acid drugs' molecular weight using chosen techniques, and analyze results with specialized software for accuracy.

Verification and Reporting

Validate analysis results, perform reproducibility tests for reliability, and compile a detailed report with methodology, data, and conclusions.

Our Technology Platforms

Platforms	Descriptions
HPLC Platform	HPLC is utilized for determining nucleic acid drug molecular weights through effective separation and identification via suitable columns, mobile phases, and gradients.
MS Platform	Mass spectrometry, often paired with liquid chromatography, provides precise molecular weight data, enhancing sensitivity and accuracy for nucleic acid drug detection using methods like ESI.
Gel Electrophoresis Platform	Gel electrophoresis (agarose or polyacrylamide) separates nucleic acid fragments by size, offering rough molecular weight estimates, though less precise than mass spectrometry.

Our Advantages of Nucleic Acid Drug Molecular Weight Analysis Services

By employing advanced instruments and techniques, our nucleic acid molecular weight analysis provides highly accurate measurement results, ensuring reliable quality control and research.
Many services offer rapid analysis cycles, significantly reducing the time from sample submission to result acquisition, facilitating time management in the R&D process.
Our services handle a variety of sample forms and sources, including synthetic oligonucleotides and naturally extracted RNA or DNA samples, meeting diverse research needs.
We provide professional analysis reports and data interpretation support, helping clients understand and apply molecular weight analysis data to accelerate research progress and innovation.

To ensure optimal performance of nucleic acid drugs during development and application, CD Formulation provides detailed molecular weight analysis services. Utilizing advanced technology and instruments, we accurately assess and characterize the molecular weight properties of nucleic acid drugs, offering researchers and developers reliable data support to aid in in-depth research and product optimization. If you have related needs or inquiries, please feel free to contact us.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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