Lipid-based Nanoparticle Development

CD Formulation excels in crafting lipid nanoparticles, a sophisticated delivery system aimed at safeguarding nucleic acid medications and boosting their absorption and precision. This approach improves upon traditional delivery techniques by fine-tuning lipid recipes and nanoparticle designs, presenting a strong option for nucleic acid-based treatments in clinical settings.

Designing Nucleic Acid Delivery Lipid Nanoparticles

Cationic lipids typically feature an ammonium-bearing hydrophilic head, which can become positively charged in acidic conditions through interaction with hydrogen ions. This electrostatic attraction facilitates the encapsulation of nucleic acids within the lipid nanoparticles. Once encapsulated, the nanoparticle's exterior is hydrophobic due to the outward orientation of the cationic lipids' hydrophobic ends. Introducing PEG-lipids, commonly used in traditional liposome synthesis with a PEG-modified end, allows the PEG-lipids' hydrophobic end to bind with the cationic lipids' hydrophobic ends. The PEG-lipids' hydrophilic end then turns outward, forming the exterior shell of the nucleic acid lipid nanoparticles. To enhance stability, cholesterol or similar substances may be added to tighten the association between the hydrophobic segments of PEG-lipids and cationic lipids, yielding the final nucleic acid lipid nanoparticles.

Fig.1 Schematic diagram of nucleic acid delivery lipid nanoparticles. (CD Formulation)

Exploring Our Lipid Nanoparticle Development Services

CD Formulation focuses on providing lipid nanoparticle development services, employing cutting-edge technology and scientific methodologies to create efficient and safe nano drug delivery systems for clients.

Calculating N/P Ratio in Nucleic Acid Delivery LNPs

In synthesizing nucleic acid lipid nanoparticles, the ratio of positive to negative charges influences the stability and properties of the nanoparticles like charge and zeta potential. The positive charge typically comes from cationic lipids with ionizable amine groups (N), while the negative charge is from nucleic acids carrying numerous phosphate groups §, allowing them to bind electrostatically. An improper ratio may lead to issues such as excessive particle size or poor stability, making it essential to correctly calculate the N/P ratio. In practical applications, one can establish the N/P ratio first and determine the concentration of the organic or aqueous phase by reverse calculation if needed.

Characterization of LNPs

Our Workflow of Lipid-based Nanoparticle Development

Fig.2 Flow chart of lipid-based nanoparticle development. (CD Formulation)

• Environmental Preparation

Before creating lipid nanoparticles, ensure all lab supplies, reagents, and the workspace are RNase-free to protect the integrity of nucleic acid materials.

• Initial Mixing

Mix lipids in ethanol with an acidic aqueous buffer containing oligonucleotides. This step encourages the self-assembly of lipids to encapsulate nucleic acids by adjusting pH and other conditions.

• Mixing Dynamics

Utilize appropriate mixing equipment to finely control the binding efficiency of lipids and nucleic acids, resulting in LNPs with uniform and stable physical properties.

• LNP Formation

After mixing, preliminary LNPs are formed. Adjust processing parameters to refine particle morphology and physical properties, adapting them to future application needs.

• Post-Processing and Purification

Conduct further processing to remove unencapsulated materials and by-products. Techniques like size exclusion, ultracentrifugation, or dialysis ensure high-purity LNPs suitable for research applications.

Our Technology Platforms

Our Advantages of Lipid-based Nanoparticle Development

• We offer flexible design solutions to meet specific requirements and specifications of nucleic acid formulations.
• Utilizing cutting-edge technology ensures efficient delivery and stability, guaranteeing high-quality products.
• We possess efficient production capabilities to meet various project scales and ensure prompt delivery.
• Our systems accommodate multiple types of nucleic acid formulations, showcasing adaptability and broad application potential.

Publication Data

CD Formulation comprises a seasoned team dedicated to delivering high-quality lipid nanoparticle development services, providing comprehensive support for your research and development projects. For further details or collaboration inquiries, please contact us anytime.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.