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Folic Acid-siRNA Conjugate Development

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CD Formulation develops FA-siRNA conjugates using nanotech and molecular bio for improved siRNA delivery. Expertise in chem synthesis and bio formulation allows creation of conjugates with targeted binding for stable transport, aiding gene silencing research.

Benefits of Folic Acid-siRNA Conjugates

siRNA is capable of cytoplasmic expression, enabling quick action and adaptable drug delivery. This capability has accelerated progress in gene therapy technologies. Folic acid directs itself to tumors by binding to folate receptors, facilitating the entry of siRNA conjugates into cancer cells and suppressing oncogene activity. The complex formed with siRNA enhances compatibility with biological systems, minimizes harmful effects, and boosts efficiency in gene delivery via chemical modification.

Fig.1 Advantages of FA-siRNA conjugates. Fig.1 Advantages of folic acid-siRNA conjugates. (CD Formulation)

Explore Our Folic Acid-siRNA Conjugate Development Services

CD Formulation provides a complete service from design to validation, ensuring quality throughout.

Items	Descriptions
Folic Acid-siRNA Conjugate Development	The development of folic acid-siRNA conjugates involves chemically binding folic acid to siRNA sequences for specific cellular recognition. This requires precise molecular design to ensure effective fusion and achieve the desired biological function.
Folic Acid-PEG-siRNA Conjugate Development	In the folic acid-PEG-siRNA complex, polyethylene glycol (PEG) enhances molecular stability and delivery efficiency, allowing for effective siRNA delivery while reducing degradation in the extracellular environment. This design emphasizes molecular coordination to optimize performance for the intended application.
Folic Acid-PEI-siRNA Conjugate Development	Folic acid-PEI-siRNA conjugates were developed by conjugating folic acid to polyethyleneimine (PEI) for targeting folate receptor-positive cells. siRNA was electrostatically bound to PEI, creating a stable nanocarrier that improved cellular uptake and enabled targeted release to tumor cells via specific folate binding.

Our Workflow of Folic Acid-siRNA Conjugate Development

Fig.2 Development flowchart of FA-siRNA conjugates. Fig.2 Flow chart of folic acid-siRNA conjugate development. (CD Formulation)

Conjugate Design

Based on the results of the needs analysis, design an appropriate strategy for the binding of folate and siRNA. This involves selecting the suitable chemical linkage and optimizing the molecular structure to enhance stability and functionality.

Synthesis and Validation

Chemically synthesize FA-siRNA conjugates and confirm their structure and purity. Verify that the products align with design specs using LC-MS and other methods.

Functional Testing

Conduct a series of experiments to verify the biophysical properties and functions of the conjugates. This includes cellular experiments to assess the distribution of the conjugates and their specific binding affinity to target molecules.

Evaluation and Optimization of Results

Based on the test results, the design of the complex is evaluated and optimized as needed. Ensure that it meets the project objectives and demonstrates strong application potential.

Our Technology Platforms

Platforms	Descriptions
Solid-Phase Synthesis Platform	Solid-phase synthesis technology enables efficient synthesis of siRNA, enhances stability, and facilitates targeted delivery in the development of folic acid-siRNA conjugates.
Enzymatic Synthesis Platform	Enzymatic synthesis platforms can be utilized to assemble siRNA strands or to conjugate folic acid to siRNA, thereby enhancing the safety and biocompatibility of the resulting conjugates.
Analytical Platform	Analytical technologies such as NMR, HPLC, and MS are employed to monitor and assess the quality and functional activity of folic acid-siRNA conjugates. These methods also help optimize the synthesis process and ensure robustness and utility.

Advantages of Folic Acid-siRNA Conjugate Development Services

The enhanced structure of the complex helps to maintain the stability of siRNA in vivo and prevents degradation.
Folic acid, when modified with functional groups, can be conjugated with other functional entities to improve overall performance.
The mature synthesis process during development can reduce development time and control costs.
With the introduction of folic acid, the conjugates are more readily internalized by cells, enhancing the uptake rate.

Published Data

Technology:Folic acid-conjugated siRNA via chemical synthesis

Journal: Nucleic acid therapeutics

IF: 4.244

Published: 2021

Results:

This overview highlights FA's role in siRNA delivery for cancer and inflammation treatment, detailing various FA-conjugated nanoparticles such as liposomes, silica, metals, dendrimers, and more. It aids in crafting delivery systems targeting siRNA to tumors via folate receptors.

Fig.3 Structures of FA-siRNA conjugates. Fig.3 Structures of folic acid-siRNA conjugates. (Gangopadhyay S, et al., 2021)

CD Formulation employs advanced technologies to create folic acid-siRNA conjugates, improve delivery efficiency through nanotechnology and molecular biology, ensure stable and targeted delivery, and foster innovation in gene silencing. Contact us to learn more.

References

Gangopadhyay S, Nikam R R, Gore K R. Folate receptor-mediated siRNA delivery: recent developments and future directions for RNAi therapeutics. NUCLEIC ACID THER. 2021, 31(4): 245-270.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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