Custom piRNA Synthesis Services

CD Formulation offers leading custom piRNA synthesis services. Leveraging advanced technology platforms and a professional R&D team, we provide precise and reliable piRNA solutions to scientists and research institutions worldwide, helping you achieve groundbreaking progress in genomic research.

What is piRNA (Piwi-interacting RNA)?

piRNA are unique small non-coding RNAs, consisting of 26 to 31 nucleotides, that interact closely with Piwi proteins. They predominantly exist in the germ and stem cells of animals and are vital for these cells' growth and preservation. Their structure includes a 5' end with single phosphate uracil and a 3' end with 2'-O-methylation, showcasing specific expression patterns in tissues. Biologically, piRNA play essential roles in gene silencing, the upkeep of germline and stem cell functions, as well as the regulation of protein synthesis and mRNA stability, thus being pivotal in maintaining genome stability and aiding in cell differentiation.

Functions of piRNA

• Gene Silencing

piRNAs are small RNA molecules that play a key role in silencing genes within eukaryotic cells, especially crucial in inhibiting transposons.

• Transposon Control

piRNAs, through their association with Piwi proteins, can locate and dismantle transposon RNAs, preventing the instability these elements might introduce into the genome.

• Epigenetic Modulation

They contribute to changes in epigenetics by influencing the patterns of DNA methylation and the modification of histones.

• Genome Stability

By attaching to repetitive DNA sequences, piRNAs help preserve the structural consistency of the genome.

Explore Our Custom piRNA Services

Solid Phase Synthesis Technology for piRNA

Solid phase synthesis technology is used for piRNA preparation by sequentially constructing the nucleotide chain on a solid support for efficient and accurate synthesis. Initially, the first nucleotide is attached via its 3' end to the solid support, securing it for subsequent reactions. Then, deprotection and coupling reactions are performed sequentially, using activators to add new nucleotides to the 5' end of the growing chain, maintaining precise chemical control to ensure sequence integrity. After completing the assembly of all nucleotides, protective groups are removed, and the piRNA is cleaved from the support and purified by HPLC to remove impurities, resulting in high-quality piRNA for research and application.

piRNA Analysis and Characterization

Considerations for Customizing piRNA

• Ensure precise target sequence design, utilizing bioinformatics tools to predict potential off-target effects.
• Choose high-purity reagents, especially key in coupling and modification steps, to improve synthesis success rate.
• Adjust conditions like temperature, pH, and ionic strength as necessary to optimize reaction efficiency.
• Employ appropriate purification techniques such as HPLC to ensure product purity and homogeneity.
• In functional testing, evaluate the binding capability of piRNA to targets and its activity in cell models.

Our Technology Platforms

General Workflow for piRNA Preparation

Fig.1 Flow chart of piRNA preparation. (CD Formulation)

• Target Identification and Design

Conduct a thorough analysis of the target genomic area using bioinformatics tools to design piRNA sequences capable of precise target binding, ensuring specificity and efficacy in subsequent experiments.

• Sequence Synthesis

Employ the latest nucleic acid synthesis technology in the laboratory to synthesize the designed piRNA sequences and perform appropriate chemical and structural optimization based on preliminary experiments to enhance stability and bioactivity.

• Validation and Modification

Synthesized piRNA needs to undergo biological function validation to ensure its efficacy in cells or model systems.

• Production and Quality Control

Large-scale production of piRNA is based on a comprehensive quality control system to ensure batch consistency and product safety.

Our Advantages of piRNA Preparation Services

• We deliver an all-inclusive suite of services, from preparing samples to analyzing data, guaranteeing our clients a streamlined and effortless experience.
• Our adept bioinformatics team ensures precise and reliable data insights.
• With state-of-the-art techniques, we maximize piRNA enrichment, surpassing typical market data yields.
• By continually innovating technology and refining processes, we enhance synthesis efficacy and data integrity, strongly supporting clients' research endeavors.

Publication Data

At CD Formulation, we pride ourselves on delivering top-tier custom piRNA synthesis services that support your pioneering research in genomic science. Our advanced platforms and dedicated team ensure precision and excellence at every step. For bespoke solutions and to discuss your specific project needs, please get in touch with us.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.