Custom CpG Oligodeoxynucleotide Synthesis

CD Formulation's goal is to provide you with the most affordable and high quality CpG oligodeoxynucleotides synthesis services utilizing state-of-the-art equipment, advanced technology, and an experienced team of professionals.

What are CpG Oligodeoxynucleotides (CpG ODNs)?

CpG ODNs (CpG oligodeoxynucleotides) are synthetic DNA sequence with unmethylated cytosine-guanine pairs that mimics the immunostimulatory properties of bacterial DNA. As TLR9 agonists, they activate immune cells such as B cells and plasma cell dendritic cells, triggering both immediate and long-term immune responses. Their thiophosphate skeleton enhances stability and cellular absorption, making them more durable than natural bacterial DNA.

Explore Our Custom CpG ODNs Synthesis Services

CD Formulation has the experience and expertise to help you prepare high-quality CpG ODNs such as Type A, Type B and Type C ODNs to reach your research goals sooner.

Class A CpG ODNs Customization Services

Class A is a palindromic sequence with a PTO-based CpG and a poly-G tail at the 3' end. Class A CpG ODNs do not activate B cells but stimulate plasmacytoid dendritic cells (pDCs) to secrete interferon-alpha. Modifications can enhance their stability and immunostimulatory effects.

Class B CpG ODNs Customization Services

Class B includes fully phosphorothioate-refined linear CpG ODNs, some of the oldest identified. This class stimulates B cells without activating pDCs to release IFN-. Customization generally involves adjusting sequence length and PTO modification for stability and enhanced immunostimulatory effects. Additional modifications may target the CpG motif and backbone to achieve specific immunogenicity titers for therapeutic applications like vaccine design.

Class C CpG ODNs Customization Services

Class C includes the structures of classes A and B, with a pure PS backbone and a CpG palindrome motif. It stimulates B cells and pDCs to release interferon-alpha (IFN-), but its effect is less pronounced than that of class A or B ODNs. Sequence modifications can help balance immune activation and reduce toxicity, which is essential for personalizing treatments for diseases like cancer or chronic infections.

Our Workflow for CpG Oligodeoxynucleotides Preparation

Fig.1 Flow chart of CpG Oligodeoxynucleotides preparation. (CD Formulation)

• Sequence Selection

Appropriate CpG sequences are selected based on the available literature and databases. Typical CpG sequences consist of unmethylated CpG dinucleotides.

• Chemical Synthesis

The solid phase synthesis method can be utilized to prepare oligonucleotides with various modifications such as phosphate and phosphodiester modifications as desired.

• Quality Assessment

The molecular weight and structure of CpG ODNs are analyzed using techniques such as mass spectrometry (MS) and nuclear magnetic resonance (NMR).

• Bioactivity Testing

Our ELISA technology platform can be used to perform bioactivity analysis of CpG ODNs, including immunogenicity analysis.

Our Technology Platforms for CpG ODNs Synthesis

Solid-phase Synthesis Technology

CD Formulation provides solid-phase synthesis technology for customization of CpG ODNs, and we can develop proprietary solutions for our clients based on their needs, applications, and more.

Analytical Technology Platforms

• MS Platform

The Mass Spectrometry (MS) platform determines the molecular weight and consistency of CpG ODNs.

• HPLC Platform

HPLC platform is used to purify and measure the concentration of CpG ODNs.

• NMR Platform

NMR Platform employs NMR and UV spectroscopy to assess the structure and stability of CpG ODNs.

• ELISA Technology

ELISA can measure biological function of many kinds of nucleic acids and our technology platform is very sensitive, fast-detection and accurate.

Advantages of CpG ODNs Synthesis Services

• Customized sequence design meets specific customer needs, ensuring optimal biological activity and specificity.
• We use advanced solid-phase synthesis technology to produce high-purity oligonucleotides for diverse applications.
• We accommodate various needs, from small research to large commercial production, and can adjust synthesis batch sizes as needed.
• Various chemical modifications can improve the stability and targeting of CpG ODNs to meet specific needs.

Publication Data

CD Formulation offers tailored synthesis services for CpG Oligodeoxynucleotides (ODNs) using advanced technology and a committed team to ensure top-tier quality. Reach out to us for more information about our offerings and see how we can support your project needs.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.