Yeast Expression Technology

Yeast protein expression technology is ideal for the large-scale production of proteins with a variety of post-translational modifications with well-defined genetic characteristics. CD Formulation integrates yeast expression technology into our internal protein production platform, offering the ultimate solution for your protein production. Our scientists have decades of experience and can provide you with high-quality, considerate yeast expression solutions that will effectively save you costs and time.

What is The Yeast Expression Technology?

Yeast, the simplest eukaryotic expression system, is an excellent host for the production of functional recombinant proteins. It is easy to culture, low-cost and can be produced on a large scale while supporting post-translational modifications and correct folding of eukaryotic proteins. The system is also capable of producing membrane proteins and many recombinant transmembrane proteins. Common yeast expression hosts for the expression of recombinant proteins include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe.

Fig. 1 Schematic of yeast expression vector. (Khatibi P, et al., 2014)

Our Services Related to Yeast Expression Technology

CD Formulation is one of the global leaders in the production of therapeutic proteins using yeast expression technology. Our yeast expression technology-based therapeutic protein production services cover all steps from DNA synthesis to vector construction to protein production. Our scientists optimize codons according to the codon preferences of different yeasts and improve protein production by considering gene dosage, integration site, transformant phenotype, and culture conditions.

Our services include but are not limited to:

Codon Optimization and Gene Synthesis (Optional)

Virus Generation

• The synthesized gene was cloned into a suitable expression vector.
• Plasmid sequencing and large-scale plasmid preparation.

Identification and Screening of High-Expression Strains

• PCR determined positive transformants.
• High-copy transformants were screened for target protein expression.

Protein Expression Analysis

Small-scale expression tests were performed on selected monoclonal strains.

Expression and Purification

• Small-scale expression, purification, and optimization of expression conditions.
• QC analysis: SDS-PAGE, UV, etc.

Large-scale Expression and Purification (Optional)

• A large-scale expression and purification according to the selected appropriate expression conditions.
• QC analysis: SDS-PAGE, UV, etc.

Target Protein Identification

• Identify the protein using qualitative or quantitative analysis methods.
• Purified proteins (can be lyophilized).

Available Yeast Expression Vectors

Yeast Integrating Plasmid (YIp)

YIp integration vectors cannot replicate autonomously and must be integrated into the genome by low-frequency homologous recombination. Usually, YIp vectors are integrated as a single copy. For overexpression of specific genes, the YIp plasmid has two yeast fragments, YFG1 and URA3 markers, which can be integrated at any genomic site.

Yeast Centromeric Plasmid (YCp)

YCp vectors are autonomously replicating vectors with a centromere sequence (CEN) and an autonomously replicating sequence (ARS). The copy number of YCp vectors is very low, only 1 to 3 per cell. Since these vectors are relatively unstable, they are not suitable for high-level expression, but can be used as routine cloning vectors.

Yeast Episomal Plasmid (YEp)

The YEp vector is autonomously replicative. Since the fragment of the yeast 2 μm plasmid is the origin of replication, it is also responsible for the high copy number and high transformation frequency. Generally, this system is suitable for small-scale expression studies and is not recommended for large-scale production.

Available Yeast Expression Option

Our scientists commonly use Saccharomyces cerevisiae and Pichia pastoris expression systems for high-efficiency production of heterologous proteins.

Custom Yeast Expression Services

Features of Yeast Expression Technology

• Rapid growth and relatively simple culture conditions make it suitable for large-scale fermentation production.
• Compared with other eukaryotic systems (such as mammalian cells), yeast culture and operation are relatively simple and low-cost.
• Complex post-translational modifications.
• Easy to precisely modify the yeast genome.
• Easy to purify and separate for subsequent proteins.
• Non-pathogenic microorganisms, high safety.

Publication

Thanks to our in-house advanced yeast expression platform, CD Formulation is committed to providing efficient and economical production of therapeutic proteins. Please feel free to contact us if you are interested in our services. Learn how our yeast expression technology can support your recombinant protein synthesis projects.  

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.