Yeast Expression System-Based Therapeutic Protein Production

The yeast expression system combines the advantages of prokaryotic and higher eukaryotic systems and is an important tool for the production of therapeutic proteins. As a trusted protein production contract organization, CD Formulation is committed to providing customers with high-quality protein production services. We have rich experience in host cell resources and vector selection, which can effectively increase cell density and protein production.

What is The Yeast Expression System?

The yeast expression system is one of the commonly used systems for eukaryotic protein expression. It has the advantages of a short prokaryotic expression cycle, low cost, and easy operation, and is easy to scale up for industrial-level culture. At the same time, it has the ability of eukaryotic cells to fold proteins spatially and modify glycosylation. The system clones the target gene into an appropriate expression vector and then transforms it into yeast cells, using its efficient transcription, translation, and subsequent modification capabilities to synthesize the target protein. Since the yeast expression system can be cultured on a large scale and is relatively low in cost compared to mammalian cells, it is suitable for large-scale protein production. Commonly used yeast species are Saccharomyces cerevisiae and Pichia pastoris. Saccharomyces cerevisiae has good genetic tools and mature genetic engineering technology, while Pichia pastoris is widely popular for its high-density fermentation ability and efficient protein expression.

Fig. 1 Schematic representation of engineered yeast secreting humanized therapeutic proteins. (Madhavan A, et al., 2021)

Explore Our Yeast Expression System-Based Therapeutic Protein Production Services

As a leading protein production contract organization, CD Formulation is focused on providing high-quality therapeutic protein production services to customers around the world. Our professional team has extensive experience in providing customized solutions for a wide range of biopharmaceutical products.

Our yeast expression system-based therapeutic protein production services cover all steps from DNA synthesis to vector construction to protein production. Our scientists optimize codons according to the codon preferences of different yeasts and improve protein production by considering gene dosage, integration site, transformant phenotype, and culture conditions.

Codon Optimization and Gene Synthesis (Optional)

Virus Generation

• The synthesized gene was cloned into a suitable expression vector.
• Plasmid sequencing and large-scale plasmid preparation.

Identification and Screening of High-Expression Strains

• PCR determined positive transformants.
• High-copy transformants were screened for target protein expression.

Protein Expression Analysis

Small-scale expression tests were performed on selected monoclonal strains.

Expression and Purification

• Small-scale expression, purification, and optimization of expression conditions.
• QC analysis: SDS-PAGE, UV, etc.

Large-scale Expression and Purification (Optional)

• A large-scale expression and purification according to the selected appropriate expression conditions.
• QC analysis: SDS-PAGE, UV, etc.

Target Protein Identification

• Identify the protein using qualitative or quantitative analysis methods.
• Purified proteins (can be lyophilized).

Available Yeast Expression Systems

Features of Yeast Expression System

• Rapid growth and relatively simple culture conditions make it suitable for large-scale fermentation production.
• Compared with other eukaryotic systems (such as mammalian cells), yeast culture and operation are relatively simple and low-cost.
• Complex post-translational modifications.
• Easy to precisely modify the yeast genome.
• Easy to purify and separate for subsequent proteins.
• Non-pathogenic microorganisms, high safety.

Our Different Expression Platforms for Therapeutic Protein Production

Why Choose Us for Therapeutic Protein Production Using Yeast Expression System?

• Our team consists of experts with extensive experience in protein production, fermentation technology, and yeast biology.
• We have successfully developed and produced many therapeutic proteins using yeast systems.
• We excel in producing complex proteins that require specific glycosylation patterns using cutting-edge yeast expression systems such as Pichia pastoris and Saccharomyces cerevisiae.
• We use optimized expression and purification protocols to obtain high yields of therapeutic proteins with minimal impurities.
• We provide scalable therapeutic protein production solutions, from small-scale laboratory experiments to large-scale production for clinical trials and commercialization.
• Our optimized protocols reduce production time, speed up the development process, and bring your product to market faster.

Publication

Thanks to our in-house advanced yeast expression platform, CD Formulation is committed to providing efficient and economical production of therapeutic proteins. Please don't hesitate to contact us if you are interested in our services. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.