Therapeutic Peptide Biosynthesis

Biosynthetic approaches can exploit the natural machinery of organisms to fold and modify peptides to produce products that are closer to their native form. CD Formulation uses biosynthesis technology to provide advanced recombinant peptide synthesis services to customers around the world. These recombinant peptides can be designed and synthesized according to the customer's custom specifications, ensuring that each peptide sequence is accurate and meets the specific needs of their project.

About Therapeutic Peptide Biosynthesis

Peptide biosynthesis embodies a fascinating confluence of molecular biology and cutting-edge genetic engineering, wherein the creation of recombinant peptides unfurls through the intricate machinations of genetically altered organisms. At the crux of this process lies the imaginative act of introducing a meticulously crafted DNA sequence, one that holds the blueprint for the desired peptide, into the genetic tapestry of a host organism. This is no mere transplant, it often involves the deft maneuvering of a plasmid or a similar vector, acting as a molecular chariot to ferry the genetic instructions into the very heart of the microbial or cellular realm.

Fig. 1 Production of peptides using DNA recombinant synthesis. (Heymich M L, et al., 2021)

Explore Our Therapeutic Peptide Biosynthesis Services

Enzymatic Decomposition Synthesis Method

We use biological enzymes to degrade large molecular animal or plant proteins into small molecular peptides. The peptides obtained by enzymatic hydrolysis retain the original activity of the protein and have more functions than the original protein.

This method has a mild reaction and is usually used to prepare short peptides.

Recombinant Synthesis Method

This method is used to create various peptides and uses cloning and gene expression in microorganisms as two key elements to produce peptides. This method is a viable alternative for developing new peptides.

In the recombinant expression method, our scientists use a variety of different expression platforms, including bacterial systems (Escherichia coli and Bacillus subtilis), yeast systems (Saccharomyces cerevisiae and Pichia pastoris), and mammalian or insect cell lines to synthesize target peptides.

Genetic Engineering Method

We have developed a genetic engineering method based on DNA recombinant technology to synthesize peptide sequences by selecting appropriate DNA templates to produce active peptides. Our genetic engineering method is low-cost and has a strong expression orientation of synthetic peptides.

Recombinant Peptide Synthesis Workflow

Recombinant peptide synthesis refers to the production of peptides through recombinant DNA technology.The following is our general workflow for producing recombinant peptides:

• Design and construction of expression vectors.
• Expression and purification of recombinant fusion peptides.
• Proteolysis and purification of recombinant peptides.

Our Solutions for Therapeutic Peptide Biosynthesis

Short peptides are easily degraded by microbial proteases. In E. coli, we use two different approaches to address this problem:

• Using protease-deficient strains, the peptides are produced as insoluble inclusion bodies or concatemers. This strategy protects the peptides from protease activity and protects the expression host from the toxic effects of the peptide. It is more suitable for the expression of short peptides without complex secondary structures.
• Producing peptides as tandem repeats separated by cleavable residues. This strategy also protects these peptides from protease activity and improves yield. These multimers are often fused to molecular tags in vivo to increase their stability. Common tags include His tags (for affinity purification), thioredoxin (TrxA), small ubiquitin-like modifier (SUMO), and self-cleavable intein tags.

Biosynthetic Methods Highlights

• Lower Cost of Large-Scale Production: Recombinant synthesis is more cost-effective than chemical synthesis because the culture medium required is much cheaper than synthetic amino acids. Therefore, it is an efficient method to produce large quantities of peptides in an industrial process.
• Promote 3D Protein Folding and Post-Translational Modifications: Recombinant technology can produce peptides with complex tertiary structures and specific post-translational modifications, which are necessary for biological activity and drug efficacy.
• Better Sustainability: Compared to chemical synthesis processes, the use of biological systems will reduce chemical waste and energy consumption, thereby promoting green manufacturing principles and reducing the environmental impact of the process. However, purification processes may reduce these benefits.
• Long-chain Peptide Production Capability: Compared to synthesis methods such as solid-phase peptide synthesis (SPPS), recombinant peptide synthesis is extremely efficient, even when the chain length reaches hundreds of amino acids, by utilizing organisms and their ribosomal machinery.

Peptide Manufacturing & Analytical Services

In addition to peptide synthesis capabilities, CD Formulation combines flexible GMP manufacturing facilities with cutting-edge peptide analytical knowledge to provide a full range of quality control testing services to accelerate the commercialization of your products, including:

Publication

CD Formulation has extensive experience in peptide biosynthesis. Each synthesis step is subject to our strict quality control. Please don't hesitate to contact us if you are interested in our services. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.