Peptide Antigens Synthesis

Peptide antigens are a viable alternative to using full-length proteins as immunogens, as the use of relatively short peptides to mimic specific parts of a target protein has been shown to have many advantages in a variety of situations. CD Formulation has leading expertise in the design and synthesis of complex peptide antigens, specializing in the synthesis of custom peptide antigens for antibody production and custom antibody generation. We can meet your needs for conformationally restricted peptide constructs, incorporation of PTMs (e.g., phosphorylation or sulfation), or conjugation to carrier proteins (KLH, BSA, etc.).

What is Peptide Antigen?

Peptide antigens are short chains of 8 to 30 amino acids that can trigger an immune response in the body. These peptide antigens are usually derived from proteins and represent fragments of larger proteins or polypeptides. During the immune response, peptide antigens are received by antigen-presenting cells (such as dendritic cells and macrophages), processed, and presented to T cells in the form of peptide-major histocompatibility complex (MHC) complexes. This process is crucial for identifying foreign pathogens, cancer cells, and other abnormal cells. By designing specific peptide antigens, the body can be induced to produce an immune response against specific pathogens or tumor cells, thereby effectively improving the targetedness and efficiency of treatment.

Explore Our Custom Peptide Antigen Synthesis Services

At CD Formulation, our team of peptide synthesis experts has the expertise and specific skills to design and synthesize high-quality peptide antigens for a variety of applications based on your needs.

Our scientists are familiar with a variety of synthesis techniques, including solid-phase peptide synthesis (SPPS), liquid-phase peptide synthesis (LPPS), and chemo-enzymatic peptide synthesis (CEPS), allowing us to select the most appropriate technology to support each individual peptide synthesis project. At the same time, we strictly follow quality control standards to ensure that we provide you with high-purity, low-impurity peptide products.

Our unique and proven protein mimicry technology combined with our comprehensive expertise in peptide synthesis allows the creation of high-quality peptide antigens for monoclonal antibody generation, including (if required) conformational restrictions, PTMs, conjugation or biotinylation.

Our peptide antigen synthesis services include but are not limited to:

• Modified peptide synthesis: Provide different chemical modifications, including phosphorylation, methylation, acetylation, etc., to study the impact of post-translational modifications (PTMs).
• Conformational restricted peptides: Design and synthesize peptides with structural restrictions to enhance the affinity and specificity of antibody binding.
• Conjugate construction: Conjugate peptides to other molecules (such as fluorescent dyes, enzymes, or drugs) to construct functional peptide derivatives.
• Biotinylated peptides: Provide biotinylated peptides for easy conjugation with streptavidin for downstream biological detection and analysis.
• Peptide splicing: Construct large peptides or proteins by splicing multiple peptide segments, suitable for complex antigen design.
• Custom peptide synthesis: Synthesize peptides of various lengths and sequences according to your specific needs.

Design Your Peptide Antigen

Although many peptide sequences may be immunogenic, not all are equally effective in generating antibodies reactive against the target protein. We focus on the following factors during the peptide antigen design process to ensure that you are satisfied with the product you synthesize.

• Accuracy of the amino acid sequence of the target protein.
• Predicted higher-order structure of the intact protein.
• Protein domain/region mimicked by the peptide antigen.
• Ease of synthesis of certain peptide sequences, ideally, the peptide should be 10 amino acids or more in length.
• Avoid any cysteine residues whenever possible.

Available Peptide Antigen Options

Our peptide antigen synthesis service provides the following two peptide antigen production methods, including:

Multi-Antigenic Peptides (MAP)

MAP is a branch of synthetic peptides that have a backbone core of lysine residues to support the formation of a variety of peptide sequences with less than 8 branches. The molecular weight of these peptides (10kDa-15kDa) is ideal because they are highly immunogenic without the need for conjugation to a carrier protein. Our MAP synthesis method is standard SPPS and includes:

• Anchoring Boc-Lys(Boc)-OH to the resin.
• Sequential treatment with TFA and deprotection.
• Coupling and deprotection cycles.

Note that for the extreme C-terminal region of the protein, we recommend the use of peptide-protein conjugates.

Peptide-Carrier Protein Conjugation

Peptide-carrier protein conjugates are another class of peptide antigens that are useful when the epitope is located at the C-terminus of the peptide. In the peptide-protein conjugation process, the peptide is bound to the carrier protein by covalent or non-covalent bonding to form the conjugate peptide.

Peptide Manufacturing & Analytical Services

In addition to peptide synthesis capabilities, CD Formulation combines flexible GMP manufacturing facilities with cutting-edge peptide analytical knowledge to provide a full range of quality control testing services to accelerate the commercialization of your products, including:

Publication

CD Formulation's peptide antigen synthesis services are tailored to meet the specific needs of researchers and organizations seeking high-quality peptides for drug discovery, vaccine development, diagnostics, and other scientific endeavors. Please don't hesitate to contact us if you are interested in our services. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.