Multi-angle Light Scattering (MALS) Technology

Multi-angle light scattering (MALS) is a non-invasive, rapid technique for the absolute characterization of proteins, polymers, and nanoparticles. CD Formulation has extensive experience in developing various MALS methods, such assize exclusion chromatography-MALS (SEC-MALS), ion exchange chromatography-MALS (IEX-MALS), and field flow fractionation-MALS (FFF-MALS). We utilize state-of-the-art MALS technology to provide reliable analytical support for the development and characterization of protein/peptide drugs.

What is Multi-angle Light Scattering (MALS)?

MALS is a unique and powerful technique for determining the absolute molar mass and size of proteins in solution. MALS detectors detect a wide range of sizes and masses and are able to characterize protein molecules as monomers, dimers, or higher-order aggregates. The technique is a detection technique based on scattered light to characterize macromolecules and particles. The amount of scattered light depends, among other things, on the molecular weight and size of the macromolecule or particle.

Multi-angle Light Scattering (MALS) Principle

The principle of the MALS detector is based on measuring the static intensity of laser light scattered at different angles by macromolecules in solution passing through a flow cell. It provides the same thermodynamically rigorous molar mass values ​as a sedimentation equilibrium experiment in an analytical ultracentrifuge (AUC) but in a much shorter time. To obtain meaningful information about heterogeneous samples, MALS must be combined with a separation device, such as a gel filtration column (SEC-MALS) or a FFF separation device (FFF-MALS).

Fig. 1 Illustration of multi-angle light scattering. (Shamir M, et al., 2019)

Our Services Related to Multi-angle Light Scattering (MALS)

With decades of experience supporting protein/peptide biopharmaceutical development and manufacturing, our team of highly qualified experts can provide a range of MALS-related services to accelerate the implementation and success of your project.

MALS detection technology is applicable to compounds with a molecular weight of about 1,000 g/mol and a size of about 500 nm and above. It is often used in conjunction with chromatographic techniques (SEC and AF4) to measure the molar mass and size of eluted compounds.

At CD Formulation, our scientists use MALS detectors in combination with size exclusion chromatography (SEC), field flow fractionation (FFF), and other techniques to measure the absolute molecular weight, size, and conformation of protein/peptide macromolecules in solution and analyze the absolute molar mass and size of macromolecules in solution to gain insight into their structure, aggregation, and interactions.

Our MALS technology supports all stages of your protein/peptide drug development and manufacturing—from early research to downstream process monitoring and GMP batch release testing.

• Measurement of native molecular weight and size distribution of proteins and various macromolecules.
• Protein/peptide oligomerization studies.
• Protein/peptide interaction and aggregation studies.
• Analysis of protein conjugates, and nanoconjugates.
• Characterization of protein conformation and aggregates.

Our Equipment

• Size Exclusion Chromatography With MALS (SEC-MALS)
• Gel Permeation Chromatography With MALS (GPC-MALS)
• Ion Exchange Chromatography With MALS (IEX-MALS)
• Field Flow Fractionation With MALS (FFF-MALS)
• Composition Gradient With MALS (CG-MALS)

Advantages of Multi-angle Light Scattering (MALS) Technology

• Molar mass range: 1 Da to 1 GDa.
• Molecular size range (rg): 10 to 500 nm.
• High sensitivity to high molar mass components and aggregates.
• Excellent for solving problems that SEC and AF4 can't handle.

Custom Multi-angle Light Scattering (MALS) Services

Why Choose Our Multi-angle Light Scattering (MALS) Technology?

• We have a team of experts with rich experience in performing MALS analytical method development and validation.
• We have accumulated decades of expertise and successful project experience using MALS technology to support protein/peptide biopharmaceutical development.
• Our laboratories are equipped with state-of-the-art equipment and technologies to support any type of protein characterization.
• We provide flexible experimental design and customized solutions.
• Fast turnaround time: detailed technical reports are provided within 5-7 days.

Publication

CD Formulation aims to provide a non-invasive and powerful analytical tool for the absolute characterization of proteins and their nanoformulations. Please feel free to contact us if you are interested in our services. Learn how our MALS technology can support the smooth implementation of your protein/peptide biopharmaceutical program.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.