Microwave-Assisted Solid-Phase Peptide Synthesis (MW-SPPS) Technology

The use of microwave energy to enhance solid-phase peptide synthesis has proven to be a valuable technology. It has been shown to enhance the deprotection and coupling steps in each amino acid cycle. CD Formulation uses advanced microwave-assisted solid-phase peptide synthesis (MW-SPPS) technology to optimize the synthesis of peptides, improving both the efficiency and yield of peptide production and meeting the needs of different customers.

What is Microwave-Assisted Solid-Phase Peptide Synthesis (MW-SPPS) Technology?

MW-SPPS is a process that uses microwave technology to accelerate solid-phase peptide synthesis. Studies have shown that microwave irradiation significantly enhances amide bond formation in peptide synthesis. This allows for more complete coupling in less time and potentially reduces reagent overuse. At high temperatures, carbodiimide-based coupling is preferred over onium salt-based methods, which use strong bases and lead to increased epimerization.

Fig. 1 Solid-phase peptide synthesis (SPPS) reaction scheme (MW microwave irradiation). (Murray JK, et al., 2011)

Our Services Related to Microwave-Assisted Solid-Phase Peptide Synthesis (MW-SPPS)

CD Formulation is actively developing the application of microwave technology in peptide manufacturing. Our team often uses this method to process various complex peptides, allowing synthesis in a shorter time and higher yields.

MW-SPSS is a method that uses microwave radiation to increase the efficiency of peptide synthesis. This technology improves SPPS synthesis and can synthesize peptides in high yields with low racemization.

Microwave energy prevents growing peptide chains from folding or forming aggregates and promotes chemical bonding. In other words, peptides can be easily and efficiently stitched together by microwaves, allowing the synthesis of longer peptides and various modifications to the peptide chains, including the addition of sugars and other groups.

Peptide Synthesis and Manufacturing

The steps of peptide synthesis by MW-SPPS are similar to those of SPP, including:

• Preparation for SPPS.
• Amino acid addition.
• Deprotection and cleaning.
• Chain release.

Scale-up Production

We are equipped with both laboratory-scale and GMP-grade microwave solid-phase synthesis equipment. This equipment can synthesize and manufacture high-quality, competitively priced peptides for research-grade to commercial-grade use.

Our Equipment

• Automatic microwave solid phase synthesizer.
• Fully automatic microwave solid phase synthesizer (1L).
• Fully automatic microwave solid phase synthesizer (10L).
• Fully automatic microwave solid phase synthesizer (30L).

Advantages of Microwave-Assisted Solid-Phase Peptide Synthesis (MW-SPPS) Technology

• Faster reaction times, allowing for increased reaction rates by shortening homogenization heating times.
• Reduces the use of natural and unnatural amino acids.
• Better peptide yields and purity.
• More efficient for difficult-to-synthesize peptides.
• Better reproducibility.
• Increased likelihood of dehydration or racemization of certain amino acids.

Custom Microwave-Assisted Solid-Phase Peptide Synthesis (MW-SPPS) Services

Deliverables

CD Formulation's peptides are delivered as lyophilized powders and packaged in separate sample tubes according to customer requirements. To ensure the quality of the peptides, we also provide the following documents for your reference:

• HPLC/MS Test: All peptides we produce are tested by MS and HPLC to ensure the accuracy of the peptide products.
• COA Document: We provide a COA document for each peptide, including the shape and appearance of the peptide sample, delivery specifications, molecular weight, and purity.
• Solubility Test: This is optional. We provide two solubility testing services according to customer requirements: qualitative dissolution test and quantitative dissolution test.

Publication

CD Formulation is designed to provide powerful tools for handling the synthesis of peptides of varying lengths and complexities. Please feel free to contact us if you are interested in our services. Learn how our MW-SPPS technology can support your custom peptide synthesis projects.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.