Methylated Peptide Synthesis

The pharmacokinetic landscape of peptide therapeutics, intriguingly constrained as it is, presents a fascinating puzzle obstructing their apex efficacy across a spectrum of diseases. Enter the compelling strategy of N-methylation—a promising approach aimed at unlocking the latent prowess of these biomolecules. CD Formulation can provide customers with peptide methylation services according to different needs.

What is Methylation?

Methylation is an important translational modification of proteins and peptides. N-methylation is the simplest and smallest modification of a single amino acid or peptide bond. This modification can significantly improve the stability and bioavailability of peptide drug candidates and generate analogs with specific biological activities, such as enzyme inhibitors, receptor antagonists and agonists, as well as building blocks in combinatorial chemistry for screening new potential drugs.

Fig. 1 Backbone N-methylation of peptides. (Li X, et al., 2023)

Explore Our Custom Methylated Peptide Synthesis Services

CD Formulation emerges as a luminary in the boundless expanse of peptide synthesis, presenting an extraordinary array of methylated peptide synthesis services tailored for a global clientele.

In our peptide synthesis laboratory, the methylated peptide crafting typically unfolds at the N-terminus of the polypeptide chain. This process intricately weaves together multiple methyl group transfers, artfully targeting the amino acids arginine and lysine, creating a rich tapestry of molecular modifications.

Our extensive services include:

• Methylated peptide design.
• Methylated peptide synthesis.
• Methylated peptide purification.
• Methylated peptide modification.

Available Methylated Amino Acid Option

	Amino Acids	Methylated Amino Acids
Lysine Methylation
Arginine Methylation

Our Synthetic Method for Methylated Peptide

Solid-Phase Peptide Synthesis (SPPS)

During the synthesis process, our scientists first construct the peptide chain by coupling different amino acids, and then methylate specific amino acid residues by introducing methylating agents.

Liquid-Phase Peptide Synthesis (LPPS)

Suitable for shorter peptide chains. During the synthesis process, the methylating agent can be directly added to the peptide chain through a chemical reaction under appropriate time and conditions.

Enzymatic Synthesis Method

Methylation of the peptide chain is achieved through the catalytic action of an enzyme. This method usually has high specificity and selectivity, but its scope and conditions of application are limited.

Biosynthesis

Peptides containing methylation sites are synthesized in cells using recombinant technology. This method usually involves genetic engineering technology.

Peptide Manufacturing & Analytical Services

In addition to peptide synthesis capabilities, CD Formulation combines flexible GMP manufacturing facilities with cutting-edge peptide analytical knowledge to provide a full range of quality control testing services to accelerate the commercialization of your products, including:

Publication

CD Formulation has extensive experience with each methylation modification of peptides. Please don't hesitate to contact us if you are interested in our services, and to learn more about the possibilities of incorporating methyl groups into your peptides. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.