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Eukaryotic Microalgae Expression Technology

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Eukaryotic microalgae is a new platform for producing protein drugs. Microalgae expression has the characteristics of universality and good post-translational modification. A variety of protein drugs have been successfully expressed in eukaryotic microalgae, which is a transitional target for synthetic biology research. CD Formulation integrates eukaryotic microalgae technology into our internal protein production platform and is committed to transforming its advantages into efficient and safe protein drug production solutions.

What is The Eukaryotic Microalgae Expression Technology?

Eukaryotic microalgae are an ideal platform for large-scale protein production, with many advantages including rapid growth, low cost, and the ability to undergo post-translational modifications similar to higher eukaryotic organisms. In particular, species such as Chlamydomonas, Pseudomonas triangularis, and various microalgae have received much attention in biotechnology for the production of therapeutic proteins due to their unique properties.

Fig. 1 Production of therapeutic proteins in microalgae. Fig. 1 Production of recombinant and therapeutic proteins in microalgae. (Banerjee A, et al., 2022)

Our Services Related to Eukaryotic Microalgae Expression Technology

CD Formulation is one of the global leaders in the production of therapeutic proteins using advanced engineering technology of eukaryotic algae. Thanks to the innovative eukaryotic microalgae expression technology platform, we can achieve efficient expression and precise regulation of foreign genes to ensure the function and stability of target proteins.

Our services include but are not limited to:

Strain Selection and Development
Gene Cloning and Expression
Culture Optimization
Protein Purification and Characterization
Scale-up

Available Eukaryotic Microalgae Expression System

Chlamydomonas Reinhardtii Expression System

Chlamydomonas reinhardtii is the most commonly used microalgae expression system, with mature technology for genetic modification. It can perform transient and stable transformation and is capable of late glycosylation modification. It is often used to express complex recombinant proteins.

Chlorella Expression System

The genetic engineering technology of Chlorella is relatively mature and can be transformed stably and instantaneously. It can grow rapidly and reach high cell density under controlled conditions.

Phaeodactylum Tricornutum Expression System

Phaeodactylum tricornutum has established a relatively mature gene modification technology, which can achieve instantaneous and stable transformation and is suitable for the production of some functional proteins.

Available Methods for Genetic Transformation of Microalgae

Transformation Methods	Description
Bacteria-mediated transformation	Agrobacterium-mediated transformation is the most efficient method for developing microalgae due to its wide host range, inexpensive procedures, and the large amount of DNA that can be transferred.
Electroporation	Electroporation is a routine method for microalgae and microalgae nuclear transformation. Electroporation is a rapid process that allows rapid transformation of microalgae. Our scientists have extensive experience optimizing electroporation protocols to balance membrane permeability and cell viability based on the specifics of your project.
Particle Bombardment (Gene Gun)	Particle bombardment is the use of DNA-coated particles as a delivery system for exogenous genetic material. This method is effective for nuclear and chloroplast genome transformation. It has been widely used for microalgae transformation due to its simplicity and efficiency.

Custom Eukaryotic Microalgae Expression Services

Therapeutic Protein Production

Eukaryotic algae have unique advantages over traditional expression systems in terms of exogenous gene expression and regulation, translation, post-translational modification (PTM), drug delivery, and downstream separation and purification. Leverage our innovative eukaryotic algae protein expression platform for efficient and safe protein drug production.

Publication

Published Data

Technology: Eukaryotic Microalgae Expression Technology

Journal: Sci Rep.

IF: 1.78

Published: 2020

Results:

The authors engineered an innovative expression system harnessed by endogenous gene promoters, aiming to fabricate specific proteins within the unicellular microalga Chlorella. Two genes induced by nitrogen deficiency were identified using RNA-Seq of Chlorella ArM0029B grown under nitrogen deficiency conditions. These two genes were used to produce the recombinant protein, human granulocyte colony-stimulating factor (hG-CSF), in Chlorella UTEX 395 and Chlorella ArM0029B. The promoter regions of these genes were used to construct expression vectors for the hG-CSF gene. A novel signal peptide (SP) was used to produce the target protein in the culture medium. The results showed that after stable transformation of these vectors with codon-optimized hG-CSF sequences, hG-CSF polypeptide was successfully produced in the spent culture medium of transgenic Chlorella.

Fig. 2 Construction of hG-CSF expression vectors. Fig. 2 Construction of hG-CSF expression vectors controlled by CvNDI promoters. (Shin JH, et al., 2020)

Thanks to our in-house advanced eukaryotic microalgae expression platform, CD Formulation is committed to providing efficient and economical production of therapeutic proteins. Please feel free to contact us if you are interested in our services. Learn how our eukaryotic microalgae expression technology can support your recombinant protein synthesis projects.

References

Banerjee A, Ward V. Production of recombinant and therapeutic proteins in microalgae. Curr Opin Biotechnol. 2022 Dec;78:102784.
Shin JH, Choi J, Jeon J, et al. The establishment of new protein expression system using N starvation inducible promoters in Chlorella. Sci Rep. 2020;10(1):12713.

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