Eukaryotic Algae Engineering-Based Therapeutic Protein Production

Eukaryotic algae, an innovative platform for protein drug production, has unique advantages over traditional expression systems in terms of exogenous gene expression and regulation, translation, post-translational modification (PTM), drug delivery, and downstream separation and purification. As a trusted protein production contract organization, CD Formulation integrates eukaryotic microalgae technology into our internal protein production platform and is committed to transforming its advantages into efficient and safe protein drug production solutions.

About Eukaryotic Microalgae Expression System

Algae can be used as a platform for genetic engineering and have many advantages in protein production, including rapid growth, low cost, and the ability to perform post-translational modifications similar to higher eukaryotic organisms. Eukaryotic microalgae, especially species such as Chlamydomonas reinhardtii, Pseudomonas tricornutum, and various microalgae, have gained wide attention in biotechnology for the production of therapeutic proteins due to their unique properties. As an innovative protein production expression system, the eukaryotic microalgae expression system combines the characteristics of microalgae's rapid growth, strong adaptability to environmental conditions, and the ability to photosynthesize, and has attracted more and more attention from researchers. Compared with traditional cell culture, microalgae have lower cultivation costs and shorter production cycles.

Fig. 1 Production of recombinant and therapeutic proteins in microalgae. (Banerjee A, et al., 2022)

Explore Our Eukaryotic Algae Engineering-Based Therapeutic Protein Production Services

CD Formulation is one of the world leaders in the production of therapeutic proteins using advanced engineering technology of eukaryotic algae. Our innovative approach combines the unique advantages of algal systems with cutting-edge biotechnology to produce high-quality therapeutic proteins for a variety of applications. From GMP to non-GMP proteins, we focus on providing excellent results that meet your specific needs.

Using innovative eukaryotic microalgae expression systems, we are able to achieve efficient expression and precise regulation of foreign genes to ensure the function and stability of target proteins. In addition, the unique post-translational modification capabilities of microalgae enable us to produce proteins that are closer to the natural state, thereby improving the efficacy and safety of drugs.

Our services include:

Strain Selection and Development

• Screening and Selection: Identify suitable algae strains (e.g., Chlamydomonas reinhardtii, P. tricornutum, or different species of algae).
• Genome Engineering: Use CRISPR/Cas9 and other gene editing technologies to improve productivity, stress tolerance, and targeted protein expression.

Gene Cloning and Expression

• Expression Vector Design: Construct plasmids containing therapeutic genes under appropriate promoters for algal expression.
• Transformation: Implement stable or transient transformation methods to introduce expression vectors into algal cells.

Culture Optimization

• Media Optimization: Develop customized growth media to enhance algal biomass accumulation and protein expression.
• Photobioreactor Design: Design photobioreactors for optimal light penetration, mixing, and nutrient delivery to meet the needs of a specific algal species and desired protein yield.

Protein Purification and Characterization

• Extraction Technologies: Develop efficient methods to extract proteins from algal biomass to ensure maximum yield.
• Purification Technologies: Implement chromatographic methods (affinity, size exclusion, etc.) to purify therapeutic proteins.
• Characterization: Perform biochemical and biophysical analyses to validate protein structure, function, and post-translational modifications.

Scale-up

• Pilot production: Translate successful processes from bench-scale to pilot scale to assess scalability and feasibility in large-scale bioreactor systems.
• Large-scale culture optimization: Fine-tune growth conditions, nutritional strategies, and harvesting methods to produce therapeutic proteins at scale.

Our Technology Platforms

Advantages of Therapeutic Protein Production From Eukaryotic Algae

• Versatile expression system: Algae can serve as a host system for a variety of therapeutic proteins, including antibodies, enzymes, and vaccines, enabling a diverse biopharmaceutical portfolio.
• Eukaryotic algae can undergo complex modifications, such as glycosylation, which is critical for the functionality and stability of many therapeutic proteins.
• The ability to culture microalgae and macroalgae in large quantities can reduce costs compared to traditional mammalian cell systems.
• Algal cells have a lower risk of contamination by human pathogens and can provide a safer environment for the production of therapeutic proteins.
• Eukaryotic algae have rapid growth rates and high yield potential.

Why Choose Us for Therapeutic Protein Production Using Eukaryotic Algae Engineering?

• Our team has extensive experience in genetic modification and metabolic engineering of eukaryotic algae.
• We have a proven track record in producing complex therapeutic proteins, including antibodies and other biologics, using eukaryotic algae engineering.
• We are able to provide customized protein production solutions based on our customers' specific needs, ensuring that specific functional and quality standards are met.
• We strictly follow international quality standards, such as FDA and EMA requirements, to ensure the safety and efficacy of each batch of products.
• We provide scalable therapeutic protein production solutions, from small-scale laboratory experiments to large-scale production for clinical trials and commercialization.
• Our optimized protocols reduce production time, speed up the development process, and bring your product to market faster.

Publication

CD Formulation is committed to providing customers with tailor-made solutions by integrating eukaryotic microalgae technology with our advanced protein production platform. We have advanced screening, cultivation, and fermentation technologies, which can quickly respond to market needs and provide high-yield and high-purity protein products. Please don't hesitate to contact us if you are interested in our services. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.