Biotinylated Peptide Synthesis

Biotinylation of peptides is an efficient method for conjugating specific peptides to streptavidin-coated surfaces. Due to the high affinity and binding specificity of biotin, the biotinylation interaction (biotin-avidin or biotin-streptavidin) is used as the basis of laboratory research techniques for labeling, detecting, and purifying peptides. CD Formulation offers a wide range of peptide biotinylation services to meet your diverse research needs.

What is Biotinylated Peptide?

Biotinylation is the process of incorporating biotin groups or residues into biomolecules such as peptides, proteins, and other macromolecules. For decades, the biotin-streptavidin interaction has been used as the basis for other labeling techniques for labeling, detection, and purification of peptides, proteins, and other biomolecules due to its specificity and high affinity (Kd < 10 - 10 M). Biotinylation of peptides is a very successful and efficient method for binding peptides to streptavidin-coated surfaces and is frequently used to label peptides. Biotinylated peptides have the following properties:

• Biotinylation of peptides can be synthesized at the N-terminus or the C-terminus.
• Biotinylated peptides are often used in immunoassays and fluorescence-based flow cytometry.
• Flexible spacers or linkers are inserted between biotin and peptides, such as aminocaproic acid, and PEG-Spacer with different lengths.
• Introducing unnatural amino acids, or performing post-translational modifications such as methylation and phosphorylation.

Fig. 1 Methods of selective biotinylation. (Dundas CM, et al., 2013)

Explore Our Custom Biotinylated Peptide Synthesis Services

As experts in peptide modification, CD Formulation provides unrivaled peptide biotinylation services to clients worldwide. With our robust synthesis procedures and superior peptide analysis technologies, we can address any possible challenge in the development and production of peptide therapeutics.

In our peptide synthesis laboratory, biotinylation labeling is typically performed at the end of the polypeptide chain but can be located anywhere along the chain. Biotinylation of the N-terminus or amino terminus occurs directly on the primary terminal amino group (-NH 2 ), while biotinylation of the C-terminus or carboxyl terminus occurs on the ε-amino group of the (extra) C-terminal lysine.

Our team of peptide modification experts can synthesize peptides using a range of biotin tags as well as custom peptide synthesis using a range of other tags.

Available Biotin Label Types Include:

Biotin N-Terminal Labeling	Biotin (Lysine ) C-Terminal Labeling	Biotin (EDA) C-Terminal Labeling

Design Your Biotinylated Peptide

The key consideration for peptide biotinylation is to have enough spacer between the biotin group and the amino acid to ensure the desired interaction between the peptide and the macromolecule. Adding a spacer or linker between biotin and the peptide can effectively reduce the steric hindrance during the subsequent binding process. We offer a variety of spacers and linkers to meet specific needs.

Available Linkers or Spacers Include:

Available Biotin Analogs

The biotin-avidin or streptavidin interaction is essentially irreversible under physiological conditions. Biotin binding is resistant to pH or temperature changes, organic solvents, and denaturing agents. Therefore, we will use the following biotin analogs if you need a reversible interaction.

Iminobiotin

Iminobiotin is an analog of biotin that can be used in cases where the avidin (streptavidin)-biotin complex is slightly dissociated. This is mainly due to the replacement of its upper ring structure with a 2-imino imidazole structure, which leads to a weakened interaction with the avidin or streptavidin binding site.

Desthiobiotin

Desthiobiotin is a sulfur-free biotin analog with the advantages of a high level of specificity and is not easily oxidized. It doesn't bind as tightly to avidin and streptavidin as biotin.

Peptide Manufacturing & Analytical Services

In addition to peptide synthesis capabilities, CD Formulation combines flexible GMP manufacturing facilities with cutting-edge peptide analytical knowledge to provide a full range of quality control testing services to accelerate the commercialization of your products, including:

Publication

CD Formulation has extensive experience with each biotin modification of peptides. Please don't hesitate to contact us if you are interested in our services, and to learn more about the possibilities of incorporating biotin into your peptides. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.