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Baculovirus-Insect Cell Expression Technology

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The baculovirus-insect cell expression system is one of the most powerful and robust methods for recombinant protein production and can be used to produce a variety of therapeutic proteins. As a trusted protein production contract organization, CD Formulation is committed to developing and optimizing the baculovirus-insect cell expression system to meet the diverse needs of our customers.

What is The Baculovirus-Insect Cell Expression Technology?

The baculovirus expression vector system (BEVS) is a powerful expression tool for target genes (such as SF9 and SF21) in insect cells. The target gene can be introduced into insect cells by transfection, replicated in large quantities in the cells, and finally released the recombinant protein. It has the characteristics of low cost, simple operation, and high expression, and is one of the widely used methods in biotechnology research and applications.

Fig. 1 Baculovirus expression vectors. Fig. 1 Versatility of baculovirus expression vectors. (Kost TA, et al., 2005)

Our Services Related to Baculovirus-Insect Cell Expression Technology

CD Formulation is one of the global leaders in the production of therapeutic proteins using baculovirus-insect expression technology. Our team provides high-quality therapeutic protein production services with optimized insect expression systems, covering a full range of services from gene cloning, and viral vector construction to cell culture and protein purification.

Our services include but are not limited to:

Codon Optimization and Gene Synthesis (Optional)

Virus Generation

Generate recombinant bacmid DNA and transfect it into insect cells.
Generate low titer and high titer stocks and determine virus titer by quantitative PCR.
Verify the protein expression of low titer and high titer generations using western blot.
Infect insect cells with high titer stocks.

Expression and Purification

Small-scale expression.
Pilot expression.
Purification and condition optimization.
QC analysis: SDS-PAGE, Western blot, LC-MS/MS, etc.

Large-scale Expression and Purification (Optional)

A large-scale expression and purification according to the selected appropriate expression conditions.
QC analysis: SDS-PAGE, Western blot, LC-MS/MS, etc.

Target Protein Identification

Identify the protein using qualitative or quantitative analysis methods.
Purified proteins (can be lyophilized).

Available Insect Cell Options

Insect Cells Lines	Source	Culture medium	Characteristics
SF9 Cell	SF9 cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB–21-AE.	SF9 cells are suitable for suspension culture in ESF 921 or ESF AF and are often used to produce recombinant bacilli and proteins.	Suitable for a wide range of recombinant proteins. Grows well in adherent/suspension cultures.
SF21 Cell	SF21 cell line is derived from the ovarian cells of Spodoptera frugiperda.	These cells are suitable for suspension culture in ESF 921.	Suitable for a wide range of recombinant proteins. Grows well in adherent/suspension cultures.
Tni Cell	Tni cell line is derived from the ovarian cells of Trichoplusia ni.	Tni cells are suitable for suspension culture in ESF 921 or ESF AF.	The protein content is 5-10 times that of the Spodoptera frugiperda cell line. Grows well in adherent/suspension culture.
S2 Cell	S2 cell line is derived from a primary culture of Drosophila embryonic stage 20-24 hours.	These cells are suitable for suspension culture in ESF 921.	Suitable for a wide range of recombinant proteins. Grows well in adherent/suspension cultures.
Hi5 Cell	Hi5 Cell are a clonal isolate derived from the parental Trichopulsia ni cell line (cabbage looper ovary).	These cells are suitable for suspension culture and serum-free medium and are often used for the production of heterologous proteins.	Very suitable for expressing secreted recombinant proteins. Grows well in adherent culture but forms irregular monolayers. Provides higher secretory expression than Sf9 cells.

Custom Baculovirus-Insect Cell Expression Services

Therapeutic Protein Production

Baculovirus-insect cell expression has the advantages of efficient expression of foreign proteins and the ability to perform complex post-translational modifications, which is suitable for the production of a variety of biopharmaceuticals, vaccines, and industrial enzymes. Leverage our innovative baculovirus-insect cell protein expression platform for efficient and safe protein drug production.

Features of Baculovirus-Insect Cell Expression Technology

Powerful promoter polyhedra and high titer enable large-scale protein expression.
Since baculoviruses are naturally non-infectious to humans, they have high biosafety.
Easy to expand.
Low culture cost and nutrient requirements for the culture medium are generally less complex than mammalian cells.
Multiple genes can be expressed simultaneously.
It is suitable for expressing complex proteins, including multi-subunit protein complexes and membrane proteins.

Publication

Published Data

Technology: Baculovirus-Insect Cell Expression Technology

Journal: Curr Protoc.

IF: 0.0

Published: 2022

Results:

The authors unveil an intriguing methodology for the synthesis of target proteins, intricately adorned with site-specific chemical modifications, leveraging a baculovirus/insect cell expression framework. First, a target protein containing a C-terminal thioester is prepared by intein cleavage induced by the addition of a thiol reagent (MESNA) using a baculovirus/insect cell expression system. The protein thioester is then reacted with an N-terminal Cys-containing peptide via expressed protein ligation (EPL) to generate the intact, full-length target protein. Finally, this protocol is exemplified by generating the protein kinase Akt1 with C-terminal site-specific phosphorylation for use in kinetic assays.

Fig. 2 The semisynthesis of proteins recombinantly expressed in insect cells. Fig. 2 Overview of steps described in this protocol for the semisynthesis of proteins recombinantly expressed in insect cells using expressed protein ligation (EPL). (Butts M, et al., 2022)

Thanks to our in-house advanced baculovirus-insect cell expression platform, CD Formulation is committed to providing efficient and economical production of therapeutic proteins. Please feel free to contact us if you are interested in our services. Learn how our baculovirus-insect cell expression technology can support your recombinant protein synthesis projects.

References

Kost TA, Condreay JP, Jarvis DL. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat Biotechnol. 2005;23(5):567-575.
Butts M, Chu N. Utilizing a Baculovirus/Insect Cell Expression System and Expressed Protein Ligation (EPL) for Protein Semisynthesis. Curr Protoc. 2022;2(1):e348.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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