Baculovirus-Insect Cell Expression System-Based Therapeutic Protein Production

The baculovirus-insect cell expression system is one of the most powerful and robust methods for recombinant protein production and can be used to produce a variety of therapeutic proteins. This system can express proteins more efficiently than other expression systems with better post-translational modifications. As a trusted protein production contract organization, CD Formulation is committed to developing and optimizing the baculovirus-insect cell expression system to meet the diverse needs of our customers. We have advanced technology platforms and rich experience to efficiently produce a variety of recombinant proteins, including vaccines, therapeutic proteins, and enzymes.

What is The Baculovirus-Insect Cell Expression System?

The baculovirus-insect cell expression system is an important technology for recombinant protein production. This system has the advantages of efficient expression of foreign proteins and the ability to perform complex post-translational modifications, which is suitable for the production of a variety of biopharmaceuticals, vaccines, and industrial enzymes. Baculovirus can introduce target genes into insect cells through transfection, replicate in large quantities in the cells, and finally release recombinant proteins. It has the characteristics of low cost, simple operation, and high expression level, and is one of the widely used methods in biotechnology research and applications.

Fig. 1 Baculovirus genome editing and the expression of foreign proteins. (Hong M, et al., 2022)

Explore Our Baculovirus-Insect Cell Expression System-Based Therapeutic Protein Production Services

As a leading protein production contract organization, CD Formulation is focused on providing high-quality therapeutic protein production services to customers around the world. Our professional team has extensive experience in providing customized solutions for a wide range of biopharmaceutical products.

With our optimized insect expression system, our team provides high-quality therapeutic protein production services, covering a full range of services from gene cloning, and viral vector construction to cell culture and protein purification.

In addition, with optimized expression vectors and our extensive experience in therapeutic protein expression and purification, we use the baculovirus-insect cell system to provide customers with high-quality protein expression service.

Codon Optimization and Gene Synthesis (Optional)

Virus Generation

• Generate recombinant bacmid DNA and transfect it into insect cells.
• Generate low titer and high titer stocks and determine virus titer by quantitative PCR.
• Verify the protein expression of low titer and high titer generations using western blot.
• Infect insect cells with high titer stocks.

Expression and Purification

• Small-scale expression.
• Pilot expression.
• Purification and condition optimization.
• QC analysis: SDS-PAGE, Western blot, LC-MS/MS, etc.

Large-scale Expression and Purification (Optional)

• A large-scale expression and purification according to the selected appropriate expression conditions.
• QC analysis: SDS-PAGE, Western blot, LC-MS/MS, etc.

Target Protein Identification

• Identify the protein using qualitative or quantitative analysis methods.
• Purified proteins (can be lyophilized).

Available Insect Cell Line Options

We have a variety of commonly used insect cell lines, such as Sf9, Sf21, Hi-5, etc. In general, Sf9 cells are used for virus amplification, while Hi-5 cells are used for protein expression. By optimizing expression vectors, we can effectively improve protein expression levels.

Features of Baculovirus-Insect Cell Expression System

• Has similar post-translational modification capabilities as mammalian cells, such as glycosylation, phosphorylation, etc.
• Easy to expand.
• The culture cost is low, and the nutrient requirements for the culture medium are usually not as complex as those of mammalian cells.
• It is suitable for expressing complex proteins, including multi-subunit protein complexes and membrane proteins.
• Has high safety.

Our Different Expression Platforms for Therapeutic Protein Production

Why Choose Us for Therapeutic Protein Production Using Baculovirus-Insect Cell Expression System?

• Our team consists of highly skilled scientists with extensive experience in protein expression and purification using the baculovirus-insect cell system.
• We have a proven track record in utilizing the baculovirus-insect cell system to produce complex therapeutic proteins, including antibodies and other biologics.
• Our team provides tailored solutions to meet specific protein expression, purification, and characterization requirements, ensuring alignment with your research goals.
• We utilize the latest technologies and approaches, including state-of-the-art bioreactors and purification techniques, to ensure optimal performance and results.
• We provide scalable therapeutic protein production solutions, from small-scale laboratory experiments to large-scale production for clinical trials and commercialization.
• Our optimized protocols reduce production time, speed up the development process, and bring your product to market faster.

Publication

Thanks to our in-house advanced baculovirus-insect cell expression platform, CD Formulation is committed to providing efficient and economical production of therapeutic proteins. Please don't hesitate to contact us if you are interested in our services. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.