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Acetylated Peptide Synthesis

Inquiry

N-terminal acetylation can remove the positive charge of the peptide and increase the stability of the peptide against enzymatic degradation at the end of the peptide chain. CD Formulation can provide customers with peptide acetylation services according to different needs. Our common services include the design, synthesis, and purification of acetylated peptides.

What is Acetylation?

Acetylation, a fascinating chemical transformation, inextricably weaves an acetyl group (-COCH₃) into the very fabric of a peptide chain at its amino terminus (the elusive N-terminus). This modification, oh so pivotal, opens new avenues in the realm of peptide synthesis, allowing researchers to craft peptides adorned with an acetylated N-terminus. Acetylated peptides mimic internal peptide sequences better than peptides with free termini, which can be advantageous in cell studies or in vivo experiments.

Fig. 1 N-acetylation mechanisms. Fig. 1 Schematic outline of N-terminal and lysine protein acetylation. (Ree R, et al., 2018)

Explore Our Custom Acetylated Peptide Synthesis Services

CD Formulation emerges as an expert in the realm of peptide synthesis, delivering top-tier acetylated peptides to a global clientele.

In the intricate tapestry of our peptide synthesis, the act of acetylation waltzes to the N-terminus of the polypeptide chain. This delicate modification, while it may ostensibly diminish the peptide's stability and invite potential solubility quandaries, paradoxically casts a favorable light on the stability of the molecule. The act of N-terminal acetylation engenders a structural mimicry that is harmoniously aligned with natural proteins, thus enhancing the peptide's pragmatic stability in the tumultuous landscape of biochemical interactions.

Our acetylated peptide synthesis services include but are not limited to:

Acetylated Peptide Design

In the labyrinthine realm of peptide design, our seasoned experts embark on an intricate analysis of peptide sequences, unraveling the complexities inherent in crafting acetylated peptides that challenge conventional synthesis methodologies. The design solutions we proffer are not merely superficial tweaks, they involve a nuanced manipulation of the peptide structure. We can introduce hydrophilic residues to enhance solubility, or even orchestrate a symphony of alterations by substituting one or more residues to create a harmony of stability and function.

Acetylated Peptide Synthesis

Our cutting-edge platform operates at the intersection of automation and advanced microwave technology. Our adept scientists possess the acumen to oscillate seamlessly between LPPS and SPPS, each choice pivotal, each technique a brushstroke in the masterpiece of peptide creation. Flexibility is our mantra, allowing us to navigate the peptide synthesis with dexterity and precision.

Acetylated Peptide Purification

Our team enriches acetylated peptides by affinity purification and reduces the interference of high-abundance acetylated histone peptides by subcellular fractionation.

Our Synthetic Method for Acetylated Peptide

Method	Features
Protecting Group Method	Protective groups are used to protect the amino and carboxyl groups of amino acids, first synthesizing the peptide chain and then acetylation in the last step. N-fluorobenzyl and TBDMS are commonly used protective groups.
Enzyme Catalysis Method	Acetylation of the target peptide using specific enzymes e.g., transacetylase under appropriate conditions, which has the advantages of high selectivity and mild conditions.
Chemical Modification Method	The synthesized peptide is chemically modified, for example, an acetylation agent is directly reacted with the peptide to acetylate it.

Peptide Manufacturing & Analytical Services

In addition to peptide synthesis capabilities, CD Formulation combines flexible GMP manufacturing facilities with cutting-edge peptide analytical knowledge to provide a full range of quality control testing services to accelerate the commercialization of your products, including:

Peptide identification (ESI-MS).
Peptide Molecular weight determination.
Peptide sequencing.
Peptide quantification/peptide content determination.
Peptide purity and impurity analysis (HPLC/UV).
Amino acid sequence.
Amino acid composition determination.
Net peptide content.

Enantiomeric purity testing (GC/MS; LC).
Residual counterion testing (e.g. TFA).
Elemental analysis.
Residual solvent testing.
Water content testing (GC or KF).
Peptide solubility testing.
Peptide stability testing.

Optical rotation determination.
Bioburden testing(TAMC/TYMC).
Bacterial endotoxin testing.
Sterility testing.
Cytotoxicity testing.
Process/product related impurity testing.
Other pharmacopoeia testing.

Publication

Published Data

Technology: In Situ N-acetylation Approach

Journal: Org Biomol Chem.

IF: 2.9

Published: 2014

Results:

The authors ingeniously crafted and meticulously refined a novel general approach for N-acetylation, tailoring it specifically for solid-phase synthesis. This innovative method was subsequently employed across a spectrum of peptides and a myriad of non-peptide entities, each replete with diverse functional groups. The method selected malonic acid as a precursor and the reaction formed an active ketene intermediate in situ at room temperature. Compared with known acetylation methods, the reaction has a higher yield, is independent of the structure, conformation, and sequence of the acetylated molecule, and can produce thermodynamically stable acetylated products through the ketene intermediate.

Fig. 2 Peptide N-acetylation. Fig. 2 The general protocol for peptide N-acetylation. (Chandra K, et al., 2014)

CD Formulation has extensive experience with each acetylation modification of peptides. Please don't hesitate to contact us if you are interested in our services, and to learn more about the possibilities of incorporating acetylated groups into your peptides. We look forward to cooperating with you.

References

Ree R, Varland S. & Arnesen, T. Spotlight on protein N-terminal acetylation. Exp Mol Med. 2018; 50: 1-13.
Chandra K, Roy TK, Naoum JN, et al. A highly efficient in situ N-acetylation approach for solid phase synthesis. Org Biomol Chem. 2014 Mar 28;12(12):1879-84.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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