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Structural Characterization for Nucleic Acid Drugs

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The effectiveness of nucleic acid drugs, like Aptamers, relies on the spatial conformation of the target nucleic acid molecule. CD Formulation specializes in developing and characterizing pharmaceutical formulations and offers high-quality nucleic acid structural determination services to meet your needs.

Why Structural Characterization for Nucleic Acid Drugs?

Nucleic acid drugs come in a wide range of lengths and chemical modifications, which is why maximizing their therapeutic potential requires a thorough understanding of their structural properties. The development of nucleic acid drugs involves analyzing their chemical composition and conformational properties. Accurate knowledge of nucleic acid structures is essential for the rational design and optimization of these drugs. Furthermore, certain hereditary diseases result from abnormal changes in DNA or RNA structure, and analyzing nucleic acid structure can offer insights into the molecular causes of these diseases and support targeted therapy.

Fig.1 Structural characterization for nucleic acid drug Fig. 1 Analysis and design of nucleic acid structures. (Fornace M E, et al., 2022)

Explore Our Structural Characterization Services for Nucleic Acid Drugs

CD Formulation is a top global biotechnology service provider with extensive experience in nucleic acid testing, including drug characterization, physicochemical analysis, and impurity analysis. Our aim is to offer affordable, high-quality nucleic acid drug structure identification services promptly and professionally to meet your needs.

Sequence Confirmation

With our precise nucleotide sequence analysis, you can be sure that your nucleic acid drugs are genuine and accurate. Our team of scientists uses advanced methods to verify drug candidate quality and effectiveness by determining base sequences, detecting modifications, and identifying impurities.

Secondary Structure Analysis

Rely on our expertise in secondary structure analysis to study the folding patterns of your nucleic acid drugs, such as hairpin, ring, and stem structures. By analyzing secondary structures, we can assist in evaluating stability and binding properties, empowering you to make informed decisions in drug development.

Fig. 2 Secondary structure analysis Fig. 2 Secondary structure diagram. (Zadeh J N, et al., 2011)

Higher Order Structure Analysis

Gain a deeper understanding of nucleic acid drugs through our higher-order structure analysis. We focus on overall folding and long-range interactions, crucial for evaluating stability, degradation resistance, and aggregation potential, allowing you to optimize drug candidates for optimal efficacy.

Our Technologies for Nucleic Acid Drugs Structure Characterization

Our technology platform specializes in nucleic acid drugs structure characterization.

Items	Descriptions
X-ray Technology	By analyzing the light and dark reflections in an X-ray diffraction image, scientists can determine the three-dimensional positions of atoms in a nucleic acid molecule and deduce its overall structure.
Nuclear Magnetic Resonance (NMR) Technology	NMR technology can determine the sequence information and secondary structure characteristics of DNA or RNA by detecting the chemical shifts and coupling constants of hydrogen, carbon, and other nuclei in the nucleic acid molecule.
Cryo-electron microscopy (cryo-EM) technology	cryo-EM rapidly freezes biomolecule samples like DNA and RNA to preserve their natural structures, preventing dehydration and deformation under high vacuum conditions.
Hydroxyl Radical Detection Technology	This technique uses highly reactive hydroxyl radicals (-OH) to selectively break the nucleic acid backbone near the target site, producing fragments of varying lengths. Analyzing the distribution of these fragments allows for the inference of spatial conformational characteristics of nucleic acid molecules.
Dimethyl Sulfate (DMS) Technology	DMS can methylate N7-guanine and N3-adenine sites in nucleic acids, disrupting base pairing and altering nucleic acid secondary structure. Researchers use DMS-modified site distribution to assess DNA or RNA folding in solution.
SHAPE Technology	SHAPE (Selective 2′-hydroxyl acylation analyzed by primer extension) technique uses reagents like 1M7 and NAI to selectively modify the vulnerable 2'-hydroxyl group in RNA molecules, inhibiting reverse transcriptase amplification at this site. Analyzing the distribution of the modified products allows inference of the secondary and tertiary structure of RNA molecules in solution.

Why Choose Us for Nucleic Acid Drug Structure Characterization?

Comprehensive Characterization Capabilities
By utilizing advanced analytical techniques like XRD and NMR, we can thoroughly examine the secondary and three-dimensional structural characteristics of nucleic acid molecules, including base pairing, backbone conformation, spatial folding, and other essential details.
Professional Data Management
We have implemented a comprehensive data management system where all experimental data and analysis reports undergo auditing and electronic signatures.
Flexible Service Model
We offer tailored analytical method development, validation, and verification services based on customer requirements.

Publication Data

Technology: Identification of RNA structures

Journal: Nature Reviews Molecular Cell Biology

IF: 81.3

Published: 2024

Results:

Over the past decade, the development of high-throughput RNA structure analysis methods has greatly advanced our ability to map and characterize different aspects of RNA structure in whole cell populations, single cells, and single molecule transcriptomes. The resulting high-resolution data have provided insight into the static and dynamic nature of RNA structures, revealing their complexity as they fulfill their respective functions in the cell. In this review, the authors will discuss recent technological advances in determining RNA structure and the role of RNA structure in RNA biogenesis and function, including in transcription, processing, translation, degradation, localization, and RNA structure-dependent condensates. The authors also discuss the current understanding of how RNA structure guides drug design for the treatment of genetic diseases and against pathogenic viruses, and highlight current challenges and future directions for RNA structure research.

Fig.3 Identification of RNA structures Fig.3 Figure of RNA structures. (Cao X, et al., 2024)

CD Formulation specializes in determining the structure of nucleic acid drugs. Our team of dedicated scientists in drug discovery and development ensures high-quality structure determination services using specialized analytical techniques tailored to each client's needs. For further information, please contact us or submit an inquiry.

References

Zadeh J N, Steenberg C D, Bois J S, et al. NUPACK: Analysis and design of nucleic acid systems. J. Comput. Chem. 2011, 32(1): 170-173.
Fornace M E, Huang J, Newman C T, et al. NUPACK: analysis and design of nucleic acid structures, devices, and systems. 2022.
Cao X, Zhang Y, Ding Y, et al. Identification of RNA structures and their roles in RNA functions. Nat. Rev. Mol. Cell Biol. 2024: 1-18.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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