Custom Small Interfering RNA (siRNA) Synthesis

Small interfering RNAs are naturally occurring RNA molecules that are part of the non-coding RNA family found within cells. CD Formulation offers a comprehensive range of customized siRNA synthesis, including various fluorescent markers and other modifications. Currently, our backbone and base modifications include amino linkers, sulfur linkers, biotin, dabcyl, phosphate, and more.

Rational Design of siRNA

Based on the analysis of the sequence information, we have established the following rules for the rational design of siRNA.

Fig.1 Rules for the rational design of siRNA. (CD Formulation)

Explore Our siRNA Synthesis Services

Customized siRNA Synthesis

CD Formulation offers customized siRNA synthesis in various quantities tailored to our clients' silencing needs. Upon receiving the siRNA sequences from our clients, our experts promptly configure these sequences using our advanced design tools. All customized nucleic acid molecules are synthesized and manufactured according to rigorous standards and undergo quality control (QC) inspections via HPLC and MS. They are then annealed in RNase-free water and converted into double-stranded form using gel electrophoresis. Furthermore, the final siRNAs are shipped as lyophilized pellets to ensure safety and stability during transport.

Control siRNA Synthesis

Control siRNA is an essential component of comprehensive RNAi experiments, primarily including transfection controls, positive controls, negative controls, and blank controls. These control products assist customers in optimizing siRNA transfection conditions, verifying the accuracy of gene silencing experimental procedures, and conducting specific analyses of siRNA-induced gene silencing effects, ensuring the successful completion of RNAi experiments.

• Negative Control siRNA Synthesis

We offer specially designed negative control siRNAs that can be utilized to demonstrate minimal off-target effects through gene chip assays.

• Positive Control siRNA Synthesis

We offer positive control siRNAs targeting endogenous genes that have been specifically designed and experimentally validated to confirm the feasibility of the RNA interference (RNAi) process, ensuring the successful execution of RNAi experiments.

• Transfection of siRNA Synthesis

Fluorescently labeled targetless siRNAs can be effectively detected using fluorescence imaging systems. We offer Cy3 fluorescently labeled transfection control siRNA that does not require chemical modification. This control can be co-transfected with the target gene siRNA during experiments to assess transfection efficiency. Detection can be performed using fluorescence microscopy, confocal microscopy, or flow cytometry.

Chemical Modification of siRNA Synthesis

Chemical modification of RNA can significantly alter the physicochemical properties of nucleic acids, which in turn affects their function. This includes various end-labeling and intermediate modifications. Common modifications we offer include phosphorothioate backbones, 2'-O-methylation, 2'-O-fluorination, cholesterol conjugation, Cy3 and Cy5 labeling, biotin, amine, DNP, and alkyne modifications, among others.

General Workflow for siRNA Preparation

Fig.2 Flow chart of siRNA preparation. (CD Formulation)

• Target Gene Selection - Choose the target gene for gene silencing.
• Sequence Design - Design effective siRNA sequences based on the target gene's sequence.
• Specificity Verification - Ensure the designed siRNA sequences are specific within the genome to avoid off-target effects.
• siRNA Synthesis - Synthesize siRNA molecules using chemical synthesis or in vitro transcription techniques.
• Cell Transfection - Transfect the synthesized siRNA into target cells to mediate gene silencing.
• Detection and Validation - Experimentally detect the gene silencing effect and validate the siRNA's effectiveness and specificity.

Our Technology Platform

CD Formulation's technology platform offers a wide range of tools and methods essential for the research and application of siRNA in gene silencing. This platform covers all vital processes and techniques involved in the design, synthesis, delivery, and analysis of siRNA. It allows for the production of high-purity siRNA, ensuring its effectiveness and specificity.

Why Choose Us for Customized siRNA Synthesis?

• CD Formulation's siRNA products come in single-stranded and double-stranded formats.
• Improve efficiency and save time by optimizing workflows with our construction experts.
• Various methods are available for synthesizing, purifying, modifying, and labeling specialty siRNAs.
• We provide specialized off-the-shelf siRNA products for various targets, featuring modifications such as 2ʹ-O-methyl (2'-OMe), 2ʹ-deoxy-2ʹ-fluoro (2'-F), 2'-O-methoxyethyl (2'-MOE), and locked nucleic acid (LNA).

Publication Data

CD Formulation offers customized siRNA services, including the design and synthesis of siRNA targeting specific gene targets to achieve effective gene silencing. The service process covers siRNA sequence design, chemical synthesis, quality control, and functional validation to ensure that the provided siRNA has efficient and specific gene knockdown effects. Contact us for professional solutions tailored to your needs.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.