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Custom Double-Stranded RNA Synthesis

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CD Formulation provides an extensive array of services, featuring bespoke solutions for nucleic acid products and comprehensive nucleic acid preparation analysis. Our current offerings include custom dsRNA with lengths spanning from 150 to 2000 base pairs.

What is dsRNA?

dsRNA (Double-stranded RNA) consists of two complementary RNA strands, which may be part of a single molecule or derived from separate molecules. This structure allows dsRNA to interact with host proteins, thereby initiating various immune and biological processes.

Applications of dsRNA

Genetic research
Disease treatment
Pest management

Strategies for Enhancing dsRNA Production

Strategies	Descriptions
Expression Plasmids	At present, there are two primary methods for constructing (dsRNA expression plasmids. The first method involves the regulation of transcription of the same target gene using two promoters oriented in opposite directions, resulting in the annealing of the two single-stranded RNAs (ssRNAs) to form a double strand.
Promoter Strength & Classification	Biological elements serve as fundamental sequences that facilitate the assembly and regulation of gene expression systems. In these systems, the strength of the promoter is directly correlated with the transcriptional levels of downstream genes. Consequently, the utilization of stronger promoters can enhance the production of dsRNA.
Plasmid Copy Number	Increasing the copy number of dsRNA expression plasmids represents one of the strategies employed to enhance dsRNA production.
Nanocarriers	Nanocarriers are biomaterials that mitigate degradation and may enhance the uptake of dsRNA from the moment of application until its absorption.

Explore Our Custom dsRNA Synthesis Services

Custom dsRNA Synthesis

CD Formulation provides customized double-stranded RNA services to equip researchers with reliable tools for gene expression regulation. Our offerings include in vitro synthesis of positive-sense and negative-sense RNAs, annealing, and quality verification to ensure high purity and specificity. We also offer tailored design and optimization recommendations for gene knockdown, silencing, and functional studies.

Quantitative Analysis of Double-Stranded RNA

Accurate quantitation can be combined with agarose gel electrophoresis to confirm the purity and integrity of dsRNA.

UV Spectrophotometer: 260nm
Fluorescent dye: RiboGreen

General Workflow for dsRNA Preparation

Fig.1 The process of preparing dsRNA Fig.1 Flow chart of double-stranded RNA (dsRNA) preparation. (CD Formulation)

PCR Reaction

Nucleic acid sequences can be amplified using suitable primers and by PCR reactions.

Purification of PCR Products

The obtained nucleic acid sequence must undergo purification through various methods to yield a high-quality product.

In Vitro Transcription

The purified nucleic acid fragments serve as templates for RNA synthesis through in vitro transcription, typically conducted with an RNA polymerase that utilizes T7, SP6, or T3 promoters to produce single-stranded RNA (ssRNA).

Synthesis of dsRNA

The two complementary single-stranded RNAs produced are annealed under mixed conditions to form double-stranded RNA.

Purification of dsRNA

Remove impurities and single-stranded RNA from the obtained dsDNA

Our Technology Platforms

Synthetic Double-Stranded RNA Platforms

Chemical Synthesis Platform	Enzymatic Synthesis Platform
Double-stranded RNA with a specific sequence is synthesized by chemically linking nucleotides step by step.	The enzymatic synthesis platform produces dsRNA using template DNA and RNA polymerase within an in vitro transcription system.

Quality Analysis Platforms

HPLC platform and MS platform are employed to assess the purity and integrity of dsRNA.

Advantages of Custom dsRNA Synthesis Services

The sample exhibits high purity, devoid of proteins, ribonucleotide triphosphates (rNTPs), and salts.
The yield is guaranteed; you will receive the precise quantity of dsRNA that you ordered.
The provision of high-quality dsRNA at competitive prices results in significant cost savings.
The efficiency of the process is characterized by a brief lead time from the placement of the order to the delivery of dsRNA.

Publication Data

Technology: Double-stranded RNA (dsRNA) preparation by in vitro transcription synthesis technology

Journal: Nature Reviews Molecular Cell Biology

IF: 113.915

Published: 2022

Results:

dsRNA is associated with most viral infections. Nearly all organisms can recognize and respond to dsRNA to mitigate infections. Notably, recent studies have shown that vertebrate cells can accumulate self-derived dsRNA or dsRNA-like species in response to dysregulation of multiple cellular processes, activating the same immune pathways as infected cells. In the review, the authors will describe the mechanisms by which viruses or host cells produce immunostimulatory dsRNAs in mammalian cells and how cells respond to them, with a focus on recent advances in the role of cellular dsRNAs in immune regulation.

Fig.2 dsRNA signalling Fig.2 dsRNA sensors and their signalling. (Chen Y G, et al., 2022)

CD Formulation aims to deliver cost-effective, high-quality dsRNA synthesis services, ensuring client satisfaction through timely and professional execution. If you have any special requirements for our dsRNA synthesis services, please feel free to contact us and we look forward to working with you on your attractive project.

References

Chen Y G, Hur S. Cellular origins of dsRNA, their recognition and consequences. Nat. Rev. Mol. Cell Biol. 2022, 23(4): 286-301.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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