Custom Bridged Nucleic Acids (BNAs) Synthesis

CD Formulation is dedicated to developing innovative nucleic acid-based technologies that facilitate the production of compounds with exceptional binding affinity and chemical/biological stability. Through our advanced nucleic acid preparation technology, we offer tailored BNA synthesis services to address the unique requirements of researchers around the globe.

What are BNAs?

To date, a third generation of BNA (Bridged Nucleic Acid), referred to as 2', 4'-BNA-NC, has been developed. This new variant can be easily integrated into the oligonucleotide chain, facilitating the design of BNA-hybridizing oligonucleotides that meet the demand for a very high degree of sequence-specific hybridization to nucleic acids. BNA's high affinity for nucleic acids and its nuclease resistance make it an exceptional tool for the development of high-value assays and therapeutic products.

Applications of BNAs

• Antigen Gene Inhibition
• Gapmer Antisense Studies
• In Vivo and In Vitro Delivery
• RNA Interference Research
• RNA Function
• Real-time qPCR
• SNP Detection and Allele-Specific PCR
• In situ hybridization
• Deoxyribonucleases and Nucleases
• Biosensors and Beyond

Explore Our Custom BNAs Synthesis Services

Our BNA customization services encompass all stages, from custom synthesis to the final product. This includes precise purification of BNA, rigorous quality control, and flexible batch production. CD Formulation is dedicated to delivering high-quality BNA products tailored to customer needs. We ensure each batch meets functional standards through optimized synthesis and purification, while our quality control adheres to strict industry standards for purity and functionality.

BNA Oligonucleotide Synthesis

BNA oligonucleotides enhance primer and probe design flexibility. They can be combined with DNA, RNA, and other nucleic acid analogs using standard phosphoramidite synthesis. Additionally, they can be easily labeled or modified with conventional labels like DIG, fluorescent dyes, biotin, and amino junctions.

Purification of BNA

Purification of bridged nucleic acids is typically conducted using a HPLC technology platform. Our technology platform optimizes the separation of nucleic acids of varying lengths and sequences by employing different stationary and mobile phase conditions.

Quality Control of BNA

Quality control of biologically active nucleic acids (BNAs) is crucial for their development and production, ensuring efficiency and reliability. This process involves assessing product purity, verifying sequence accuracy, and analyzing physicochemical properties. Stringent quality control minimizes defects and impurities, enhancing stability and biocompatibility.

Our Workflow for Bridged Nucleic Acids (BNAs) Preparation

Fig.1 Flow chart of BNA preparation. (CD Formulation)

• Requirement Analysis

Determine the required BNA sequence, along with the necessary chemical modifications and the length of the BNA. Select the appropriate sequence based on the experimental requirements and design objectives.

• Sequence Design

Use bioinformatics tools to design a highly specific and stable oligonucleotide sequence for the intended application.

• BNA Synthesis

BNA is synthesized through chemical processes and subsequently purified to yield a highly pure product.

• Quality Control

After synthesis, rigorous quality control measures are implemented, including mass spectrometry and HPLC, to verify the purity and accuracy of the BNA.

Our Technology Platforms

Within CD Formulation's specialized BNA synthesis technology and analysis platform, we utilize several advanced techniques for precise synthesis.

Synthesis Technology Platform

Analytical Technology Platform

Highlights of Bridged Nucleic Acids (BNAs) Synthesis Services

• Through customization services, customers can obtain BNA for different research directions to meet diverse scientific needs.
• The BNA customization service can be personalized according to the specific needs of the client, be it sequence, structure or chemical modification.
• BNAs can be designed to improve binding affinity with target nucleic acids and enhance their targeting properties.
• Our team of experts can assist clients in optimizing the design to ensure that the final product achieves the desired results.

Publication Data

CD Formulation offers Bridged Nucleic Acid (BNA) customization services designed to meet diverse research and application needs. Feel free to contact us and we will provide customized solutions to assist in research and product development.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.