With a deep understanding of the importance of immune system support in animal health, CD Formulation offers expertise in assessing the immunomodulatory effects of these products. Through both targeted analyses and broad immune profiling techniques, we can generate robust data on modulatory mechanisms of action and downstream clinical impacts.
How Do We Evaluate the Immunomodulatory Function?
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Specific Immune Response |
Non-specific Immune Response |
- We aim to understand the product's ability to modulate and enhance targeted immune responses.
- Our studies analyze how the product impacts the production of antibodies, activation of specific immune cells, regulation of immune signaling pathways, etc.
- Through comprehensive in vitro and in vivo assays, we can assess the influence on the overall adaptive immune response.
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- We focus on the product's effects on the innate immune system.
- Our evaluations involve studying the modulation of key innate immune activities like phagocytosis, natural killer cell cytotoxicity, cytokine secretion and other aspects.
- We provide insightful data on properties such as immune surveillance enhancement, inflammatory response regulation and general support for immune homeostasis.
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Our Immune Care Function Testing Programs
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Immune Cell Proliferation Ability |
- Lymphocyte transformation tests using mitogens and antigens.
- Analysis of proliferation markers like Ki-67 expression and cell cycle progression using multi-color flow cytometry.
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| Natural Killer Cell Activity |
- In vitro chromium release cytotoxicity assays to quantify NK cell lytic activity against immunogenic tumor target cells like K562.
- Determination of how samples modulate cytokine production from NK cells following activation, quantifying cytokines like TNF-α, IFN-γ, IL-12 via cytometric bead array or ELISA.
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| Phagocyte Activity |
- Both in vivo and ex vivo assays to assess phagocytosis of pathogens/particles by neutrophils and macrophages via microscopic analysis or flow cytometric techniques.
- Bacterial killing assays using E. coli or S. aureus to analyze intracellular bacterial killing by macrophages/neutrophils.
- Reactive oxygen species generation and quantification in phagocytes exposed to stimuli.
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| Cytokine Secretion Function |
- Multiplex assays to obtain immune cell secretion profiles of pro/anti-inflammatory cytokines like IL-1, IL-6, IL-10, TNF, etc.
- Analysis of cytokine levels in serum/plasma samples via ELISA and Luminex technologies.
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| Serum Antibody Production |
- Antibody titer determination using ELISA methods with Fc-detection or isotype-specific secondary antibodies.
- Serum immunoglobulin subclass/isotype analysis to characterize humoral responses.
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| Lymphocytes Surface Marker Analysis |
- Multi-color flow cytometric immunophenotyping of T cells, B cells, NK cells and other immune cell subsets in spleen single cell suspensions.
- Assessment of activation markers expression like CD25, CD69 on T cells or maturation markers on B cells.
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Applications of Our Immunomodulatory Function Evaluation Services
- Development of Veterinary Health Care Products
Our testing services provide crucial data during the development phase of veterinary health products/foods. By assessing their immunomodulatory function, we can determine the effectiveness and potential benefits of these products in supporting the immune system of animals.
- Quality Control of Veterinary Health Care Products
Our testing services ensure that veterinary health products/foods meet quality standards and requirements. By evaluating their immunomodulatory effects, we can verify their consistency, efficacy, and safety, ensuring that they deliver the desired immune system support.
Highlights of Our Immunomodulatory Function Evaluation Services
- Broad portfolio of validated immunology assays, from screening to mechanism elucidation.
- Customized approach to study design that addresses customers' specific questions.
- Capabilities to efficiently evaluate products across multiple animal species.
- Data analytics experts for statistical analysis and modeling support.
- Comprehensive profiling of adaptive and innate immune parameters.
- Tissue-level assessments via histopathology and cell immunophenotyping.
Do you have a veterinary food or health product in development seeking enhanced immune benefits? Or are you looking to substantiate the immunomodulatory effects of an existing formula or therapeutic? Contact us today to discover how our tailored approach to immunomodulatory function evaluation can directly support and strengthen your projects!
