With a deep understanding of the importance of healthy aging for our animal companions, CD Formulation specializes in assessing the efficacy of veterinary supplements. Using techniques including behavioral assessments, biomarker analysis, and geriatric testing protocols, we generate robust data on how formulations may impact factors related to aging such as cognition, mobility, organ function, and cellular health.
Our Animal Models for Anti-Aging Research
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Aged Animal Models |
- 18-month-old rats or 12-month-old mice.
- The animals need to be fed for more than 12 weeks.
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| Juvenile Animal Models |
- For long-term testing, we usually use 6-month-old rats or 3-month-old mice.
- Rats and mice need to be raised for more than 12 and 9 months respectively.
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| Genetically Inherited Animal Models of Aging |
- Old (6-months-old) or young (2/3-months-old) animals of SAM aging-promoting mice.
- The animals need to be fed for more than 12 weeks.
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| Induced Aging Animal Model |
- D-galactose induced senescence animal model
- Parkinson's disease (PD) animal model
- Alzheimer's disease (AD) animal model
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Our Anti-Aging Function Evaluation Test Programs
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Brain Aging Biomarkers |
- Morris Water Maze/T-maze tasks assess hippocampal-dependent learning & memory.
- Brain tissue stained with amyloid/tau antibodies to detect protein aggregation linked to pathologies like Alzheimer's.
- Neuroinflammatory markers (IL-1β, TNF-α) analyzed from brain homogenates.
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| Liver Aging Biomarkers |
- Hematoxylin & eosin staining of liver sections scored for collagen proportionate area and lobular inflammation.
- Assays measure hepatic levels of senescence-associated β-galactosidase enzyme activity.
- Activity of cytochrome P450 enzymes involved in xenobiotic metabolism e.g. CYP1A2, CYP2E1.
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| Cardiovascular Aging Biomarkers |
- Echocardiography and electrocardiography to detect signs of left ventricular hypertrophy, QT-interval prolongation.
- Plasma/serum assays for LDL, HDL cholesterol, triglycerides, C-reactive protein (inflammation marker).
- Cardiac troponin-I concentration (cardiomyocyte injury marker).
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| Kidney Aging Biomarkers |
- Masson's trichrome histology of glomerular tuft area and mesangial matrix deposition.
- Kim-1 (kidney injury molecule-1) enzyme levels in urine samples.
- Blood urea nitrogen, creatinine, cystatin C concentrations.
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| Antioxidant Biochemical Indexes |
- Total antioxidant capacity assays from plasma/tissue samples.
- Oxidative stress biomarker analysis of various organs.
- Enzyme activity tests (SOD, G6PD, Catalase, GPx).
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Our Survival Testing Services
- C. elegans Survival Testing
We assess lifespan, reproduction, motility and pathology when fed test compounds. This high throughput model is useful for initial screening.
- Drosophila Melanogaster Survival Testing
Using flies fed test diets, we evaluate median lifespan, stress resistance, physical functioning and biomarkers to identify anti-aging pathways impacted.
We can conduct lifespan and health span studies on mice. Organs are harvested for analysis of age-related changes at biochemical, cellular and molecular levels through techniques like histology. Whole genome sequencing and transcriptomics evaluate if formulations modify longevity gene expression patterns.
Why Choose Our Anti-Aging Health Function Evaluation Services
- Multi-tiered approach utilizing both invertebrate and mammalian models to efficiently screen and validate anti-aging effects.
- Histopathological analysis identifies tissue-level anti-aging impacts.
- Multi-factorial evaluation of effects on longevity, mobility, cognition, organ function, etc.
- Biomarker analyses to elucidate anti-aging mechanisms of action.
- Molecular profiling through techniques like genomics, proteomics and metabolomics.
At CD Formulation, we offer a range of tailored evaluations to assess the anti-aging efficacy of your products. Our testing covers diverse tissues, utilizes multiple model organisms, and analyzes relevant biomarkers to provide you with reliable and meaningful data. Contact us today to learn more about how our anti-aging functional testing services can propel your veterinary health products to new heights of success!
