Nano Transdermal Formulation Encapsulation Efficiency (EE%) Testing

Effective drug encapsulation is important for enhancing the rate of drug absorption through the skin, extending the duration of drug effects, and minimizing potential side effects. CD Formulation has extensive experience in nanoformulations and can assist in conducting encapsulation efficiency tests for nanoformulations.

About Encapsulation Efficiency (EE%) Testing of Nano Transdermal Formulations

The encapsulation rate is the proportion of the drug within the total drug quantity in the nanoformulation, indicating how much of the drug is enclosed by the carrier. As the variety of nanocarrier drugs continues to grow, the range of encapsulated and free drugs within these carriers has also expanded. Consequently, there are now a variety of methods available for detecting encapsulation rates. CD Formulation offers numerous detection techniques, including low-temperature ultracentrifugation and the dextran gel column method, to cater to the diverse detection requirements of various nano-transdermal formulations.

What Can We Offer

The key to the determination of the encapsulation rate is to separate the encapsulated drug from the unencapsulated free drug, and then use spectroscopy, chromatography, and other analytical methods to detect the concentration of the encapsulated drug or free drug. We can provide you with a variety of determination methods, including but not limited to the following:

Ultracentrifugation

Use the gravity difference between free drugs and drug-containing nanocarriers for separation, which is suitable for submicron particles. This method is expensive and may affect the results due to the loss of small nanocarriers or drug leakage during the centrifugation process.

Ultrafiltration centrifugation

Combining ultrafiltration and centrifugation methods can more efficiently separate free drugs and drug-containing nanocarriers.

Dextran gel column method

Use dextran gel columns for chromatographic separation to separate free drugs and drug-containing nanocarriers according to molecular size differences. This method is often used for liposome encapsulation rate detection.

Flocculation centrifugation and protamine coagulation

The two methods have the same separation effect. The former is to add flocculants to the system to aggregate the carriers, and the latter is to use the positive charge of protamine to aggregate negatively charged or neutral carriers. For dissolved-free drugs, both methods have good separation effects.

Our Workflow for Nano Transdermal Formulation Encapsulation Efficiency (EE%) Testing

Fig.1 Flow chart of nano transdermal formulation encapsulation efficiency (EE%) testing. (CD Formulation)

1. Sample preparation: According to the type and characteristics of the nanotransdermal preparation, select the appropriate sample preparation method, such as dilution, ultrafiltration, etc.

2. Separation of free drugs from drug-containing nanocarriers: Select an appropriate separation method to ensure that free drugs and drug-containing nanocarriers can be effectively separated.

3. Drug concentration determination: Use appropriate detection methods to determine the free drug and total drug concentrations.

4. Calculate the encapsulation rate: Calculate the encapsulation rate based on the determination results and the encapsulation rate calculation formula.

Our Technology Platform

• Emulsion encapsulation rate test
• Vesicle encapsulation rate testing
• Nanoencapsulation rate testing
• Liposome encapsulation rate testing
• Drug encapsulation rate testing

Why Choose Us for Testing Encapsulation Efficiency (EE%) of Nano Transdermal Formulations?

• We have a huge database knowledge reserve and rich experience in the detection and analysis of unknown substances.
• We support a variety of test and detection schemes to ensure the accuracy and reliability of the test results.
• We provide a variety of detection methods such as ultracentrifugation and ultrafiltration centrifugation to meet the detection needs of different nano transdermal formulations.

Published Data

We provide you with comprehensive, professional, and reliable nano transdermal formulation encapsulation efficiency (EE%) testing services. If you have any needs, please feel free to contact us and our colleagues will reply to you within three working days.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.