Protein modification and labeling refers to adding or changing chemical groups after protein synthesis, or covalently attaching molecules such as biotin, fluorophores, enzymes, and radioisotopes to specific sites on target proteins. This technology has been an important tool for creating therapeutic proteins and generating novel protein structures. CD Formulation offers a wide range of protein modification and labeling services to meet the research needs of different customers.

About Protein Modification and Labeling?

The biological activity of therapeutic proteins depends largely on their amino acid composition and arrangement, correct folding and three-dimensional conformation, and the number of reactive functional groups. Protein modification and labeling technologies can introduce specific labels or make targeted modifications, such as PEGylation, glycosylation, acetylation, etc., to improve the biological activity, stability, and pharmacokinetic properties of proteins and generate more advantageous target proteins. These modification and labeling technologies can not only improve the performance of therapeutic proteins but also help researchers better understand protein-protein interactions and protein folding to study the biological functions of overall proteins.

Fig. 1 Site-Selective Chemical Protein Modification at Cys and Dha. (Boutureira O, et al., 2015)

Explore Our Therapeutic Protein Modification and Labeling Service

With the highly specialized technical capabilities in protein modification and labeling, CD Formulation can provide a variety of customized protein modification and labeling solutions. We focus on optimizing protein functionality and improving its stability and biocompatibility to meet the needs of your different research and applications.

Our team of experts will design appropriate protein modification and labeling methods based on your specific requirements to ensure that your project can proceed smoothly and achieve the desired results.

Our scientists can perform the following protein modification and labeling services, including but not limited to:

Available Protein Modification Services

• Site-Selective Chemical Modification
• Cysteine Modification
• Protein PEGylation
• Deglycosylation
• Photochemically Induced Bioconjugation
• Covalent Modification Based on Organometallic Catalysis
• Specific Modification of N and C Termini
• Protein Cross-Linking
• Non-Natural Sugar Modification
• Non-Natural Amino Acid Modification
• Disulfide Bond Modification

Available Protein Labeling Services

• Fluorescent Protein Labeling
• Protein Biotinylation
• Protein-Magnetic Bead Coupling
• Protein Quantum Dot Labeling

Complementary Analysis Services for Therapeutic Protein Modification and Labeling

To support the smooth implementation of your protein therapeutic projects, we also provide complementary protein modification and labeling analysis services. Our scientists use a variety of biochemical and physicochemical methods to determine the efficiency and specificity of protein modifications and labels. To ensure the structural and functional integrity of proteins after modification, we also perform high-resolution analyses such as mass spectrometry (MS) and nuclear magnetic resonance (NMR).

Immunoassays

Radiolabeling and western blotting are two traditional immunoassay methods we use to analyze protein modifications. Although these two methods are highly specific and relatively quantitative, they rely on prior knowledge of the modification type and the availability and specificity of the protein.

MS Analysis

MS is widely used in the biopharmaceutical industry for protein modification for quality control and batch release. This technology can not only identify protein modification types (PTMs)  but also locate modification sites without prior knowledge, such as phosphorylation, methylation, and acetylation, as well as sequence variants, including mutants and various isomers.

HPLC Analysis

We use a range of HPLC techniques to separate and purify modified proteins to assess their purity and activity.

Why Choose Our Therapeutic Protein Modification and Labeling Service?

• Our team consists of skilled professionals with extensive experience in protein chemistry, biochemistry, and biotechnology, who have a proven track record of successfully modifying and labeling therapeutic proteins for a variety of applications.
• We utilize state-of-the-art technologies, including various conjugation techniques, site-specific labeling, and post-translational modifications, for protein modification and labeling, ensuring high efficiency, specificity, and reproducibility.
• From initial project consultation to final product delivery, we offer a full range of services, including protein expression, purification, modification, labeling, and analysis. This integrated approach streamlines your workflow and accelerates project timelines.
• Our services are highly customizable, whether you are developing therapeutic proteins, antibodies, or other biologics.

Publication

At CD Formulation, we leverage our advanced technical expertise in protein modification and labeling to deliver tailored solutions that enhance protein functionality, stability, and biocompatibility. Please don't hesitate to contact us if you are interested in our services. We look forward to cooperating with you.