Proteins & Peptides Viscosity Analysis

Producing highly concentrated, low-viscosity protein or peptide therapeutics is one of the major challenges in today’s pharmaceutical industry, especially during the pre-formulation development stage, where viscosity measurement is particularly important. Accurate viscosity measurements can provide important information about protein or peptide formulations, such as differences in protein size and protein-protein interactions. CD Formulation has extensive experience in protein or peptide viscosity characterization, and our protein scientists utilize state-of-the-art analytical techniques and adhere to industry standards to ensure the highest quality of viscosity analysis.

Significance of Protein & Peptide Viscosity Characterization

The effectiveness of protein and peptide therapeutics depends largely on the active form of the protein and peptide. Only in its native form can it exert its full biological function. With the continuous development of the pharmaceutical industry, high-concentration protein solutions for subcutaneous injection have become a research hotspot.

High-concentration protein solutions are complex fluids that contain not only proteins but also surfactants, stabilizers, and salts. These components interact with each other and can affect the viscosity of the solution. In addition, beyond a certain concentration (depending on the protein), the viscosity of the protein solution may increase dramatically, leading to a series of instability problems such as aggregation, which in turn leads to the loss of the biological function of the protein. Therefore, accurate characterization of the viscosity of proteins or peptides is a key step in the protein peptide formulation development process.

Fig. 1 Vibro viscometer for protein/peptide viscosity characterization.

Explore Our Protein & Peptide Viscosity Analysis Services

The viscosity of protein or peptide solutions must be characterized to ensure their stability during development. At CD Formulation, our viscosity analysis team applies a range of advanced equipment and analytical techniques to perform viscosity measurements to further probe how formulation changes affect protein or peptide solutions. Molecular interactions are probed by introducing and quantifying changes in rheological properties when formulation changes occur.

To determine solution stability and viscosity changes, our expert team combines dynamic light scattering, microfluidics, rheology, glass capillary viscometer, small angle X-ray scattering (SAXS), rolling ball viscometer and other technical strategies to obtain accurate data on viscosity, which reflects differences in protein size and the formation of complex structures or networks caused by various types of molecular interactions.

In addition to viscosity characterization, our scientists will provide support for viscosity control of your therapeutic proteins and peptides throughout the formulation development stage, including the use of small molecule excipients (such as amino acids and salts) to reduce protein solution viscosity or reduce protein-protein interactions to reduce viscosity. A common strategy is to stabilize proteins in solution and reduce viscosity by adding arginine, an amino acid that is charged at neutral pH and whose side chain consists of a three-carbon chain with a charged guanidine group at the end.

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Applications

• Study the rheological properties of protein and peptide solutions, including parameters such as shear stress, viscosity, and elasticity, to further understand the function of proteins in vivo and their interactions with other biomolecules.
• Evaluate their stability and processing performance in industrial production, such as stability and rheological properties in pharmaceutical preparations.
• Monitor the aggregation phenomenon in protein and peptide solutions. Aggregated proteins and peptides usually have higher viscosity, which can avoid the introduction of airborne substances or impurities during the preparation and purification process, and ensure the quality and purity of proteins.
• Evaluate the interaction between drugs and proteins.

Why Choose Us for Proteins & Peptides Viscosity Analysis?

• Our team of experienced scientists are highly knowledgeable in the field of proteins and peptides viscosity analysis, and have years of experience working with various proteins and peptides.
• We have advanced technology and equipment for viscosity analysis to ensure accurate and reliable results.
• We provide flexible experimental design and testing options can meet any customer's specific needs.
• Our team is dedicated to providing exceptional customer service and support throughout the viscosity analysis process.
• Our scientists are always available to address any questions or concerns and provide ongoing assistance to our clients.

Publication

At CD Formulation, we understand the importance of viscosity analysis in the high-concentration protein solutions development and manufacturing process. Please feel free to contact us if you are interested in our services. We will provide you with the most professional advice and support to ensure the smooth launch and implementation of your project.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.