Proteins & Peptides Sequencing Analysis

Confirming the protein sequence and checking for end-terminal variations, as well as demonstrating that the primary protein sequence of the biosimilar is identical to the protein sequence of the innovator product, are requirements of the ICH Q6B regulatory guidelines. CD Formulation uses the most advanced instruments and breakthrough technology methods for protein/peptide sequencing and is committed to providing high-quality protein sequencing services to the biopharmaceutical and biosimilar industries to help customers ensure product quality and safety.

What is Proteins & Peptides Sequencing？

Protein/peptide sequencing is the process of determining the exact amino acid sequence of a protein or peptide molecule. This information is crucial for understanding the structure, function, and interactions of proteins in biological systems. The sequencing of proteins and peptides is typically carried out using techniques such as mass spectrometry, Edman degradation, and N-terminal sequencing. This information can be used in fields such as drug discovery, biomarker identification, and structural biology.

Explore Our Proteins & Peptides Sequencing Analysis Services

As a protein/peptide sequencing service provider, our scientists use mass spectrometry, gas chromatography, and Edman degradation to determine the complete sequence of proteins and peptides in protein/peptide biopharmaceutical products, including amino acid sequence and modification information.

Our protein/peptide sequencing expertise covers:

Fig. 1 Protein/peptide sequencing analysis. (CD Formulation)

• N-terminal Sequencing

N-terminal sequencing, also known as gas phase sequencing, is one of the more mature methods for analyzing protein N-terminal sequences, mainly used for protein sequencing under conditions where the N-terminus is not blocked or chemically modified.

This technology is performed by chemically removing, derivatizing, and analyzing the N-terminal amino acids in peptides or proteins in sequence. The released amino acid derivatives are then identified based on their relative chromatographic retention time relative to a group of identically derivatized amino acids.

• Mass Spectrometry Sequencing

Mass spectrometry sequencing refers to the use of mass spectrometry technology to quickly cleave peptides and determine the amino acid sequence of protein samples, which can be used to identify N-terminally blocked or modified protein samples.

• C-terminal Sequencing

C-terminal sequencing usually performed by mass spectrometry and other technologies. In mass spectrometry analysis, protein samples are first enzymatically digested by digestive enzymes to obtain amino acid fragments, and then these amino acid fragments are analyzed by a mass spectrometer to determine the C-terminal sequence of the protein.

Furthermore, our comprehensive protein/peptide sequencing services are also widely used in research such as protein/peptide identification and PTM analysis.

Our Solutions for Proteins & Peptides Sequencing Analysis

At CD Formulation, we apply mass spectrometry (LC-MS) to protein/peptide sequence identification, a technique that is able to distinguish isobaric residues.

• Our scientists can confirm sequence identity by performing mass spectrometry analysis on intact and digested peptides.
• Our scientists can identify any amino acid modifications, degradation products, and post-translational modifications by comparing the confirmed sequence to a digested reference standard.
• Our scientists are skilled in selecting appropriate protein digestion, enrichment strategies, and methods to optimize protein/peptide sequence coverage.

Our Technology Platforms

Applications

Protein/peptide sequencing is an important tool for protein/peptide biopharmaceutical development. You can choose our services for:

• Identify whether mutations and variations occur in protein/peptide biopharmaceuticals.
• Protein/peptide identity.
• Confirm the amino acid sequence of proteins, peptides, and antibodies.
• Determine the quality and purity of protein/peptide biopharmaceuticals such as monoclonal antibodies and recombinant proteins.
• Confirm whether recombinant proteins are correctly translated.
• Determine the presence and location of post-translational modifications.
• Identify and characterize proteins and peptides by comparing the obtained sequences with those in the protein database.
• Protein/peptide biosimilar similarity study.

Why Choose Us for Proteins & Peptides Sequencing Analysis?

• Our protein or peptide sequencing services are industry-leading.
• We have the most advanced industry knowledge and technology in protein and peptide sequencing.
• Our scientific analysis laboratory has decades of unparalleled expertise and successful project experience in peptide and protein sequencing.
• We provide clear and easy-to-understand project reports and internal standard runs for each sample.
• We provide flexible and tailored solutions to meet our customers' specific needs and requirements.
• Fast and efficient services ensure that our customers' projects are delivered on time.

Publication

CD Formulation understands the importance of protein sequencing in the development and production of biologics. With our experienced team and cutting-edge analytical methods, we provide tailored solutions to drive your molecule to success. Please feel free to contact us if you are interested in our services. We will provide you with the most professional advice and support to ensure the smooth launch and implementation of your project.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.