Proteins & Peptides Nanoparticles Delivery System Development

Nanoparticle delivery systems have been widely used in oral delivery of proteins and peptides. As a protein/peptide innovative delivery system solution provider, CD Formulation excels in encapsulating or surface modifying proteins/peptides in various nanoparticles, including biodegradable polymer nanoparticles, chitosan nanoparticles, solid lipid nanoparticles, and metal nanoparticles, to meet the growing demand for advanced therapeutic solutions.

Potential Advantages of Nanoparticles Delivery of Protein/Peptide

Nanoparticles refer to materials with particle sizes ranging from 1 nm to 1000 nm. They are made of synthetic or natural polymers, lipids, or metals. Since nanoparticles are more easily absorbed by cells than larger macromolecules, their use allows for faster transport to the site of action, thereby prolonging their presence in the body and limiting side effects. In addition, the use of such drug delivery systems prevents drugs from rapidly breaking down and being excreted from the body.

As innovative drug delivery carriers, nanoparticles have been widely used in the development of protein and peptide delivery systems. Potential advantages include:

• Improving bioavailability, nanoparticles can effectively reduce the enzymatic degradation and aggregation of protein and peptide drugs in the gastrointestinal environment and increase transmembrane absorption of the small intestinal epithelium.
• Change the distribution of drugs in the body.
• Achieve the therapeutic effect of controlled release and targeted treatment of diseases.
• Targeting through modified targeting ligands to prolong retention time at specific absorption sites.
• Simple preparation process.

Fig. 1 Schematic presentation of different types of nanocarrier systems. (Berillo D, et al., 2021)

Explore Our Proteins & Peptides Nanoparticles Delivery System Development Services

As an expert in protein/peptide science and formulation science, CD Formulation has the most advanced industry knowledge and technology in protein/peptide delivery and is committed to providing you with comprehensive, innovative, and efficient nanoparticle delivery system development solutions.

Our formulation science excels in encapsulating proteins or peptides in different nanoparticle carriers and achieving effective delivery and release by adjusting the properties and structure of the carriers. Our expertise covers the design and characterization of a variety of nanoparticles, including but not limited to:

• Biodegradable polymer nanoparticles.
• Chitosan nanoparticles.
• Solid lipid nanoparticles.
• Metal nanoparticles.

Our Nanoparticle Preparation Methods

Fig. 2 Categorization of methods for preparing protein nanoparticles. (Hong S, et al., 2020)

• Chemical Method

The chemical method is to prepare protein nanoparticles by chemical reactions, including emulsification and complex coacervation.

• Emulsion/solvent extraction: It is usually used to prepare polymer nanoparticles. Our scientists use mechanical stirring or ultrasonic dispersion to form an emulsified system, then remove the solvent/non-solvent to form nanoparticles.
• Polyelectrolyte complexation/complex coacervation method: Our scientists adjust the pH value to make proteins produce cations or anions, and then interact with other polymers to produce nanoparticles.

• Physical Method

The physical method is to separate proteins into nano-sized particles and then physically aggregate them, including electrospray and nano-spray drying.

• Electrospray technology: By applying high voltage to the protein solution provided by the atomizer, the liquid jet is sprayed through the nozzle to form aerosol droplets, thereby generating nanoparticles.
• Nano spray drying: The process of using heated nitrogen and carbon dioxide gases to release and dry the protein liquid jet through the nozzle to produce nanoparticles.

• Self-assembly Method

The self-assembly method is a method for preparing nanoparticles by utilizing protein self-aggregation, including the desolvation method and self-assembly method.

• Self-assembly: Our scientists dissolve individual protein chains in an aqueous solution exceeding the critical micelle concentration (CMC) and critical solution temperature (CMT) to form nanoparticles.
• Desolvation method: This method is our most commonly used method for producing protein-based nanoparticles. Our scientists synthesize nanoparticles by adding desolvation agents such as ethanol and acetone to a protein solution containing drugs.

Workflow of Proteins & Peptides Nanoparticles Delivery System Development

• Nanocarrier screening: involves screening the best nanocarrier based on the properties of your protein/peptide and clinical application.
• Nanoformulation design: involves selecting appropriate methods such as solvent precipitation, liposomes, etc. to incorporate proteins or peptides into nanocarriers to ensure stable and controlled release.
• Nanoparticle characterization and optimization: involves modifying the size, shape, or surface properties of nanoparticles using techniques such as dynamic light scattering, electron microscopy, or zeta potential analysis to enhance their stability, targeting ability, and drug release profile.
• In vitro evaluation: In vitro testing of nanoparticle delivery systems using cell culture models to evaluate their biocompatibility, targeting, and ability to deliver proteins and peptides to diseased cells.
• In vivo evaluation: Animal models are used to evaluate the biodistribution, pharmacokinetic properties, toxicity, targeting, and delivery efficiency of nanoparticle delivery systems in vivo.

Fig.3 Workflow for protein/peptide nanoparticle delivery system development. (CD Formulation)

Our Nanoparticle Manufacturing Technology

• Solvent evaporation and solvent extraction process.
• Double emulsion process.
• Phase separation.
• Spray drying.
• Spray freeze drying.
• Supercritical fluid extraction technology.

Supporting Nanoparticle Analysis and Testing Services

Our scientists perform extensive analytical testing to support the development of different protein/peptide nanoparticle delivery systems, including:

• General characterization of nanoparticles: involves the physicochemical parameters such as surface morphology, particle size, size distribution, zeta potential, crystal structure, etc., determined as CQAs during product formulation development and batch release testing.
• Entrapment efficiency and drug loading testing: involves the evaluation of the integrity of the nanoformulation, such as drug encapsulation efficiency, drug loading, and drug release rate.
• Stability testing: involves comprehensive stability studies under different stress conditions (after exposure to light, pH, and oxygen) to evaluate the degradation of proteins or peptides under different conditions.
• Biocompatibility assessment: involves the evaluation of the toxicity and biocompatibility of nanocarriers using appropriate cell models and animal models prior to clinical use to understand the physicochemical properties that may lead to adverse biological responses.

Why Choose Us for Proteins & Peptides Nanoparticles Delivery System Development?

• Extensive expertise and experience in executing protein/peptide nanoparticles delivery system development.
• We have a flexible team of experts responsible for formulation feasibility, process development, and commercial manufacturing.
• We have expertise in study design and development and validation of methods customized for the characterization of your protein or peptide nano-formulated product.
• Our team of scientists has extensive experience in the field of nanotechnology and drug delivery.
• Comprehensive analytical testing capabilities, including particle size, potentiometry, morphology analysis, stability assessment, etc.
• We provide flexible and tailored solutions to meet our customers' specific needs and requirements.
• Fast and efficient services ensure that our customers' projects are delivered on time.

Publication

CD Formulation is continually researching and developing new technologies to improve protein/peptide nanoparticle delivery systems. Our scientists stay up to date on the latest advances in the field to provide innovative solutions that can enhance protein and peptide delivery. Please don't hesitate to contact us if you are interested in our services.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.