Proteins & Peptides Liposomal Delivery System Development

One promising approach to improve protein/peptide delivery is to use liposomal delivery systems. Liposomes are lipid-based vesicles that can encapsulate and protect peptides and facilitate their transport across biological barriers, improving their stability in vivo. With strong and extensive expertise and years of experience in the field of protein/peptide delivery and liposomes, CD Formulation is committed to providing unparalleled protein/peptide liposomal delivery system development services to the biopharmaceutical industry. Our scientists have a deep understanding of the structure and properties of proteins/peptides and liposomes and can design customized delivery systems based on our customers' needs. We improve the stability and efficiency of the delivery system by optimizing the composition, size, and surface properties of the liposomes to improve their efficiency in encapsulating and releasing peptides.

Potential Advantages of Liposomal Delivery of Protein/Peptide

Liposomes are closed phospholipid bilayer vesicle systems that are able to encapsulate both hydrophilic and hydrophobic drugs, which, combined with their biocompatibility and biodegradability, make them attractive vehicles for protein/peptide delivery. In addition, it can be flexibly formed into different types of delivery vehicles by varying lipid types and ratios to meet payload requirements. Encapsulating proteins and peptides in liposomes can:

• Improve stability and protection: Liposomal delivery protects proteins and peptides from degradation by enzymes or acidic conditions in the body, thereby improving their stability and bioavailability.
• Enhanced tissue permeability: Liposomes can help proteins and peptides cross biological barriers such as cell membranes and the blood-brain barrier, allowing for better delivery to target tissues and organs.
• Controlled release: Liposomal delivery systems can be designed to release proteins and peptides in a sustained and controlled manner, prolonging their therapeutic effects and reducing the need for frequent dosing.
• Reduce side effects: By targeting specific cells or tissues, liposomal delivery minimizes off-target effects and reduces the risk of systemic toxicity associated with traditional protein and peptide therapies.
• Improved pharmacokinetics: Liposomes can enhance the circulation time and distribution of proteins and peptides in the body, thereby improving the pharmacokinetic profile and the overall efficacy of the therapeutic agent.
• Enhanced cellular uptake: Liposomes can promote the cellular uptake of proteins and peptides through endocytosis or other cellular uptake mechanisms, thereby improving their bioavailability and efficacy.
• Compatibility with different routes of administration: Liposomal delivery systems can be adapted to various routes of administration, including oral, intravenous, intranasal, and topical, providing flexibility in drug delivery options.

Fig. 1 Liposome-based nanoparticles with encapsulated protein/peptide. (Nicze M, et al., 2021)

Explore Our Proteins & Peptides Liposomal Delivery System Development Services

As an expert in protein/peptide science and formulation science, CD Formulation has the most advanced industry knowledge and technology for protein and peptide delivery and is committed to providing you with comprehensive and innovative liposome delivery system development solutions.

Our team has extensive experience and expertise and can use a variety of stabilization strategies to modify liposomes to ensure the effective delivery of proteins and peptides into target cells, including:

• PEGylation modification strategy.
• Chitosan modification strategy.
• Environmental responsiveness strategy.
• Prodrug strategy.
• Targeting strategy.
• Lyophilization strategy.

Fig. 2 Liposome modification strategies. (CD Formulation)

In addition, we will fully study the relationship between encapsulation efficiency and phospholipid composition, buffer concentration, incubation time, incubation temperature, and drug loading to provide you with the best liposome formulation and delivery system.

General Workflow for Our Proteins & Peptides Liposomal Delivery System Development

• Characterization of therapeutic proteins/peptides: including solubility, primary and higher-order structures, amino acid sequences, post-translational modifications, and aggregation analysis.
• Blank liposome formulation development: including phospholipid material selection, liposome preparation method selection, and optimization of blank liposome formulation to provide stability, controlled release, and targeting of encapsulated proteins and peptides.

• Available phospholipid materials: dipalmitoylphosphatidylcholine (DPPC), distearoylphosphatidylcholine (DSPC)...
• Available liposome preparation methods: thin film hydration method, reverse phase evaporation, ethanol injection method, supercritical fluid method, microfluidics method...

• Encapsulation of proteins and peptides: involves encapsulating proteins and peptides in liposomes using various techniques such as lipid film hydration, ultrasonic treatment, or extrusion. A high percentage of therapeutic molecules can be successfully encapsulated by optimizing the encapsulation efficiency and loading capacity of liposomes.
• Characterization of protein/peptide-loaded liposomes: involves the use of a series of analytical techniques and methods to characterize the location, structure, function, and interaction of proteins or peptides in liposomes.
• In vitro evaluation: involves in vitro evaluation of liposomal delivery systems using cell culture models to assess their cytotoxicity, cellular uptake, intracellular trafficking, and therapeutic effects.
• In vivo evaluation: involves further evaluation of liposomal delivery systems in animal models to assess their biodistribution, pharmacokinetics, tissue targeting, and therapeutic effects. These studies provide important preclinical data on the safety and efficacy of liposomal delivery systems.

Supporting Liposome Analysis and Testing Services

We offer a full range of analytical testing capabilities to support the development and manufacture of microemulsion formulations and delivery systems. All of our analytical testing capabilities can be performed in-house by our team, allowing for rapid results and close interaction between formulation and analytical teams.

• Liposome physicochemical parameter determination, including, for example, particle size, size distribution, zeta potential, and physical stability.
• Liposome drug encapsulation efficiency and drug loading determination.
• Liposome drug in vitro release evaluation.
• In vitro transdermal diffusion, in vivo skin penetration and accumulation studies (transdermal liposomes).
• Liposome residual moisture, hygroscopicity, and reconstitution analysis (lyophilized liposomes).
• Liposome cellular uptake, pharmacokinetics, and tissue distribution studies.
• Analysis of lipid (e.g. lysolipids) or drug-related degradation products.
• Residual solvent determination.
• Stability assessment of liposomal drug products.

Why Choose Us for Proteins & Peptides Liposomal Delivery System Development?

• Extensive expertise in executing protein/peptide liposomal delivery system development.
• Decades of experience in developing protein/peptide liposomal delivery systems.
• We have a flexible team of experts responsible for liposome formulation feasibility, process development, and commercial manufacturing.
• Comprehensive liposome analysis and testing capabilities, including particle size, potential, lipid content, drug encapsulation efficiency, and drug in vitro release rate.
• We provide flexible and tailored solutions to meet our customers' specific needs and requirements.
• Fast and efficient services ensure that our customers' projects are delivered on time.

Publication

CD Formulation is continually researching and developing new technologies to improve protein/peptide liposomal delivery systems. Our scientists stay up to date on the latest advances in the field to provide innovative solutions that can enhance protein and peptide delivery. Please don't hesitate to contact us if you are interested in our services.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.