Mammalian Cell Expression System-Based Therapeutic Protein Production

The mammalian cell expression system, the preferred expression platform for producing mammalian proteins with the most native structure and activity, is commonly used to produce antibodies and therapeutic proteins. CD Formulation is a trusted protein production contract organization committed to providing high-quality mammalian cell expression therapeutic protein production services. Our team has decades of experience and expertise in transient and stable expression based on HEK293 or CHO cell lines, providing customized protein production solutions based on customer needs.

What is The Mammalian Cell Expression System?

Mammalian cells (CHO cells or HEK293 cells) expression systems are one of the conventional methods for protein production. The advantage is that these cells have highly complex post-transcriptional modification mechanisms, which can effectively produce recombinant proteins with biological activity and correct folding, and are suitable for the production of antibodies, vaccines, and other therapeutic proteins. The expression system of recombinant proteins using mammalian cells can introduce appropriate protein folding, post-translational modifications, and product assembly, essential for complete biological activity. In addition, mammalian cell expression systems also have good biocompatibility and relatively complete genome modification technology, which makes them play an important role in biopharmaceuticals, basic research, and clinical applications.

Fig. 1 Schematic workflow of transient expression process in mammalian cells. (Fu Y, et al., 2024)

Explore Our Mammalian Cell Expression System-Based Therapeutic Protein Production Services

As a leading protein production contract organization, CD Formulation is focused on providing high-quality therapeutic protein production services to customers around the world. Our professional team has extensive experience in providing customized solutions for a wide range of biopharmaceutical products.

With our in-house proprietary mammalian cell expression platform, our scientists are able to efficiently produce a variety of therapeutic proteins, including monoclonal antibodies, recombinant proteins, and fusion proteins.

Codon Optimization and Gene Synthesis (Optional)

Codon optimization of target sequence according to the codon preference of CHO or HEK293.

Vector Construction

Choose the appropriate vector type according to the experimental purpose and clone the cDNA into the expression vector.

Mammalian Cell Transfection

• Use transient transfection expression to produce a small amount of protein in a short time.
• Use our proprietary stable cell line platform for long-term stable production.

Expression and Purification

• Small-scale expression, purification, and condition optimization.
• QC analysis: SDS-PAGE, UV, etc.

Large-scale Expression and Purification (Optional)

• A large-scale expression and purification according to the selected appropriate expression conditions.
• QC analysis: SDS-PAGE, UV, etc.

Target Protein Identification

• Identify the protein using qualitative or quantitative analysis methods.
• Purified proteins (can be lyophilized).

Available Mammalian Cell Line Options

We have developed proprietary mammalian cell expression platforms based on HEK293 and CHO cell lines, allowing for high-throughput delivery and high-quality recombinant protein expression.

Features of Mammalian Cell Expression System

• Capable of complex post-translational modifications, such as glycosylation, phosphorylation, carboxylation, etc.
• Has a good molecular chaperone system that can help proteins fold correctly.
• The culture conditions are relatively mild and can adapt to a wide range of bioprocessing conditions.
• The produced protein has a similar structure and function to the natural protein.
• Easily transfect recombinant DNA.
• Has a good gene expression regulation mechanism, and the expression of the target protein can be optimized through different induction conditions.
• The protein can be secreted into the culture medium and the purification process is simple.
• Stable transfection can achieve higher yields, scalability, and reproducible production.

Our Different Expression Platforms for Therapeutic Protein Production

Why Choose Us for Therapeutic Protein Production Using Mammalian Cell Expression System?

• Our team consists of experienced scientists and engineers with in-depth knowledge of mammalian cell biology and protein expression systems.
• We have completed hundreds of projects using mammalian cell expression systems to produce therapeutic proteins.
• We have a well-established mammalian cell line (e.g., CHO, HEK293) that is ideal for producing therapeutic proteins with the right structural and functional characteristics.
• We provide one-stop services from gene optimization and vector design to cell line development and large-scale production.
• We adhere to strict quality control standards throughout the production process to ensure that the therapeutic proteins produced are safe, effective, and compliant with industry regulations.
• We provide scalable therapeutic protein production solutions, from small-scale laboratory experiments to large-scale production for clinical trials and commercialization.
• Our optimized protocols reduce production time, speed up the development process, and bring your product to market faster.

Publication

Thanks to our in-house advanced mammalian cell expression platform, CD Formulation is committed to providing efficient and economical production of therapeutic proteins. Please don't hesitate to contact us if you are interested in our services. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.