Fast Protein Liquid Chromatography (FPLC) Technology

Fast protein liquid chromatography (FPLC) is a purification technique used to separate protein mixtures and collect the isolated target components. Unlike high-performance liquid chromatography (HPLC) systems, FPLC systems also use pH and conductivity monitors and fraction collectors and are a preparative rather than an analytical technique. CD Formulation integrates cutting-edge FPLC technology into our protein characterization technology platform to provide reliable analytical support for all stages of the development and production of your protein/peptide drugs.

What is Fast Protein Liquid Chromatography (FPLC) Technology?

FPLC is a liquid chromatography technique that provides high resolution via a small-diameter stationary phase and is specifically designed for the purification and separation of proteins, peptides, and nucleic acids. FPLC is similar to HPLC, but it is optimized for biomolecules and typically operates at lower pressures and flow rates, making it suitable for delicate protein structures that may be damaged at high pressures. The technique features high loading capacity, biocompatible buffer systems, fast flow rates, and stationary phases for common chromatography modes such as gel filtration, ion exchange, reversed phase, and affinity chromatography, allowing for a variety of separation and purification strategies for proteins of varying sizes and properties.

Fig.1 Fast protein liquid chromatography (FPLC) system for purifying a mixture of proteins.

Fast Protein Liquid Chromatography (FPLC) Principle

FPLC, a variant of HPLC, is specifically designed to handle biomacromolecules that are sensitive to changes in temperature, pressure, and chemical conditions, such as proteins, nucleotides, and peptides. The principle is similar to HPLC, where the composition of the eluent is changed by changing the ratio of the fluids that make up the eluent to separate proteins and peptides in the sample. The difference is that FPLC purification is usually a multi-step iterative process, and the mobile phase is a salt buffer. In addition, the system combines a variety of separation and purification strategies, including ion exchange, gel filtration, and affinity chromatography, allowing for reproducible separations, as well as multiple parameters such as UV levels, pH, and conductivity can be monitored in one run. This minimizes the time required to isolate pure proteins.

Our Services Related to Fast Protein Liquid Chromatography (FPLC) Technology

Thanks to decades of experience supporting protein/peptide biopharmaceutical development and manufacturing using FPLC technology, our team of highly qualified experts offers a range of FPLC-related services to accelerate the implementation and success of your project.

Our experienced team of experts has completed hundreds of FPLC separation and purification projects for protein and peptide, supporting all stages of your protein/peptide drug development and manufacturing - from early studies to downstream process monitoring and GMP batch release testing.

Utilizing cutting-edge FPLC technology, we support the following protein/peptide development and characterization plans, including but not limited to:

Protein/Peptide Characterization

Purity assessment: Biomolecules are separated based on their size, and the output can be analyzed by comparing peak area or peak height to standards to determine the purity of a protein sample.

Isomeric separation: Different isomers of a protein may have slightly different charge or size characteristics, and our scientists separate and characterize them by screening specific chromatographic methods.

Process Development (Analytical FPLC)

Our scientists use FPLC to develop and optimize chromatographic methods for specific proteins or peptides, and to assess the stability of proteins and peptides in various formulations.

Protein-Protein and Protein-Ligand Interaction Studies

Our scientists combine techniques such as surface plasmon resonance (SPR) or analytical ultracentrifugation (AUC) with FPLC to analyze the binding kinetics and affinity of proteins to their ligands or interaction partners.

Quality Control

Our scientists use FPLC technology to monitor the purity and consistency of protein drugs, biologics, and biopharmaceuticals to ensure regulatory standards are met and product efficacy and safety are maintained.

Advantages of Fast Protein Liquid Chromatography (FPLC) Technology

• A simple, reproducible separation technique that is highly efficient for separations but insensitive to heat-labile proteins.
• The columns are inert and can withstand very high salt concentrations and corrosive liquids.
• Wide flow range, suitable for analytical and preparative chromatography.
• FPLC supports various columns since the process is performed at low pressure.
• Excellent resolution, reproducible.

Custom Fast Protein Liquid Chromatography (FPLC) Services

Why Choose Our Fast Protein Liquid Chromatography (FPLC) Technology?

• We have a team of experts with rich experience in FPLC analytical method development and validation.
• We have accumulated decades of expertise and successful project experience using FPLC technology to support protein/peptide biopharmaceutical development.
• Our FPLC systems are equipped with the latest technology, allowing for high-resolution purification and rapid processing times. This efficiency helps accelerate your research and development timelines.
• Our rigorous method development process includes thorough validation steps to ensure reproducibility and compliance with regulatory standards, minimizing risks during your product development phases.
• We adhere to strict quality control and regulatory standards to ensure the integrity and compliance of your data.
• We provide flexible experimental design and customized solutions.

Publication

CD Formulation aims to provide a powerful analytical tool for the separation, purification, and characterization of proteins and peptides. Please feel free to contact us if you are interested in our services. Learn how our FPLC technology can support the smooth implementation of your protein/peptide biopharmaceutical program.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.